Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells

Miletić, Maja; Mojsilović, Slavko; Okić-Đorđević, Ivana; Maletic, Dejan; Puаč, Nevena; Lazović, Saša; Malović, Gordana; Milenković, P; Petrović, Zoran Lj.; Bugarski, Diana

doi:10.1088/0022-3727/46/34/345401

Cited by 51 publications

(51 citation statements)

References 51 publications

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“…These results may be utilized to optimize periodontal regeneration in the future [154]. According to Miletic et al [155], the interaction of NTAPP with the human periodontal ligament mesenchymal stem cells demonstrated that NTAPP inhibited the migration of the cells and induced some detatchment, without affecting their viability. In addition, the plasma significantly attenuated the cells’ proliferation, but promoted their osteogenic differentiation.…”

Section: Direct Plasma Applicationmentioning

confidence: 99%

Plasma in dentistry

Cha

Park

2014

Clinical Plasma Medicine

119

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This review describes the contemporary aspects of plasma application in dentistry. Previous studies on plasma applications were classified into two categories, surface treatment and direct applications, and were reviewed, respectively according to the approach. The current review discussed modification of dental implant surface, enhancing of adhesive qualities, enhancing of polymerization, surface coating and plasma cleaning under the topics of surface treatment. Microbicidal activities, decontamination, root canal disinfection and tooth bleaching were reviewed as direct applications with other miscellaneous ones. Non-thermal atmospheric pressure plasma was of particular focus since it is gaining considerable attention due to the possibility for its use in living tissues. Future perspectives have also been discussed briefly. Although it is still not popular among dentists, plasma has shown promises in several areas of dentistry and is now opening a new era of plasma dentistry.

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Section: Direct Plasma Applicationmentioning

confidence: 99%

Plasma in dentistry

Cha

Park

2014

Clinical Plasma Medicine

119

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“…Ions catalyze oxidation processes both inside and outside of the cell, which explains why they are able to have greater effect than neutral active species. [18]…”

Section: Discussionmentioning

confidence: 99%

“…[11–16] APP is known to abundantly generate radicals [17] and affect the proliferation and migration of human periodontal ligament mesenchymal stem cells. [18] Plasma can also be used without risk of contamination or secondary infection due to their bactericidal properties. [2, 19–26] To move ahead in the further development of actual commercial tools that can be used in hospitals, and in finding novel and perhaps unexpected uses of plasmas, an understanding of the mechanisms of interaction of non-equilibrium gas discharges with living organisms, tissues and cells has become essential.…”

Section: Introductionmentioning

confidence: 99%

Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells – Relevance to Capsular Opacification

et al. 2016

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Inducing selective or targeted cell apoptosis without affecting large number of neighbouring cells remains a challenge. A plausible method for treatment of posterior capsular opacification (PCO) due to remaining lens epithelial cells (LECs) by reactive chemistry induced by localized single electrode microplasma discharge at top of a needle-like glass electrode with spot size ~3 μm is hereby presented. The focused and highly-localized atmospheric pressure microplasma jet with electrode discharge could induce a dose-dependent apoptosis in selected and targeted individual LECs, which could be confirmed by real-time monitoring of the morphological and structural changes at cellular level. Direct cell treatment with microplasma inside the medium appeared more effective in inducing apoptosis (caspase 8 positivity and DNA fragmentation) at a highly targeted cell level compared to treatment on top of the medium (indirect treatment). Our results show that single cell specific micropipette plasma can be used to selectively induce demise in LECs which remain in the capsular bag after cataract surgery and thus prevent their migration (CXCR4 positivity) to the posterior lens capsule and PCO formation.

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“…17 Due to its small size and mild nature, the needle has been used for subtle stem cell manipulations as well. 18,19 Plasma sources of different designs but of very similar properties are known to be able to interact not only with the cell membranes, but the plasma effects can penetrate the cell interior and reach the DNA itself. Authors report single and double strand breaks (SSBs and DSBs) following plasma irradiation.…”

mentioning

confidence: 99%

“…The details of plasma experimental setup, gamma irradiation, primary fibroblast preparation, micronucleus test, and c-H2AX assay are explained elsewhere. 18,19,35 The effect of different kinds of irradiation is mostly based on the generation of radicals in the vicinity of DNA. 36,37 Even small concentrations of radicals can be sufficient to induce DNA damage.…”

mentioning

confidence: 99%

Plasma induced DNA damage: Comparison with the effects of ionizing radiation

Lazović

Maletic

Leskovac

et al. 2014

Applied Physics Letters

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We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2 Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei.

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Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells

Cited by 51 publications

References 51 publications

Plasma in dentistry

Plasma in dentistry

Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells – Relevance to Capsular Opacification

Plasma induced DNA damage: Comparison with the effects of ionizing radiation

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