Effects of nitroprusside on pancreatic juice secretion in the blood-perfused canine pancreas

Iwatsuki, Kazuhiko; Ikeda, Kenro; Chiba, Shigetoshi

doi:10.1016/0014-2999(82)90574-x

Cited by 17 publications

(11 citation statements)

References 17 publications

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“…A clearer dissociation of pancreatic protein secretion from PMBF has also been observed in multiple unrelated studies. For example, no change in PMBF was reported despite observing that NOS inhibitors reduce secretin-stimulated in vivo pancreatic secretion in cats (41) and that the NO donor sodium nitroprusside increases in vivo pancreatic protein secretion (17,41). To explain this dissociation between stimulated pancreatic protein secretion and PMBF, and the relationship of NO to both, Patel et al (41) proposed that NO may exert its principal effect through a nonvasodilatory mechanism, possibly by modulating vagal stimulation of secretory cells or directly stimulating acinar cells.…”

Section: Discussionmentioning

confidence: 94%

Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice

DiMagno¹,

Hao²,

Tsunoda³

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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Nitric oxide (NO) and NO synthase (NOS) play controversial roles in pancreatic secretion. NOS inhibition reduces CCK-stimulated in vivo pancreatic secretion, but it is unclear which NOS isoform is responsible, because NOS inhibitors lack specificity and three NOS isoforms exist: neuronal (nNOS), endothelial (eNOS), and inducible (iNOS). Mice having individual NOS gene deletions were used to clarify the NOS species and cellular interactions influencing pancreatic secretion. In vivo secretion was performed in anesthetized mice by collecting extraduodenal pancreatic duct juice and measuring protein output. Nonselective NOS blockade was induced with N(omega)-nitro-L-arginine (L-NNA; 10 mg/kg). In vivo pancreatic secretion was maximal at 160 pmol.kg(-1).h(-1) CCK octapeptide (CCK-8) and was reduced by NOS blockade (45%) and eNOS deletion (44%). Secretion was unaffected by iNOS deletion but was increased by nNOS deletion (91%). To determine whether the influence of NOS on secretion involved nonacinar events, in vitro CCK-8-stimulated secretion of amylase from isolated acini was studied and found to be unaltered by NOS blockade and eNOS deletion. Influence of NOS on in vivo secretion was further examined with carbachol. Protein secretion, which was maximal at 100 nmol.kg(-1).h(-1) carbachol, was reduced by NOS blockade and eNOS deletion but unaffected by nNOS deletion. NOS blockade by L-NNA had no effect on carbachol-stimulated amylase secretion in vitro. Thus constitutive NOS isoforms can exert opposite effects on in vivo pancreatic secretion. eNOS likely plays a dominant role, because eNOS deletion mimics NOS blockade by inhibiting CCK-8 and carbachol-stimulated secretion, whereas nNOS deletion augments CCK-8 but not carbachol-stimulated secretion.

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Section: Discussionmentioning

confidence: 94%

Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice

DiMagno¹,

Hao²,

Tsunoda³

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…The systemic blood pressure was monitored by a transducer (Nihon Kohden, Model RP-3). The pancreas was haemodynamically isolated in situ according to a procedure which has been previously described and illustrated diagrammatically (Hashimoto et al 1971;Iwatsuki et a/, 1982). Polyethylene cannulae were inserted into the pancreaticoduodenal and splenic arteries and used to perfuse the pancreas with the animal's own blood, conducted from the left femoral artery by means of a peristaltic pump (Harvard, Model 1210).…”

Section: Methodsmentioning

confidence: 99%

“…These inhibitory and stimulatory responses to SP on pancreatic secretion stimulated by three secretagogues caused by SP were not modified by pretreatment with atropine (100 pg), propranolol (300 pg), phentolamine (100 pg), or cimetidine (100 pg) at doses high enough to block the action of the specific agonists (Table 1). The blockers used in this study did not affect the secretagogue-induced secretion (Iwatsuki et al 1982). Bradykinin (100 ng), injected intra-arterially, caused a vasodilation and decreased the perfusion blood pressure to the same extent as SP (100 ng) (Fig.…”

Section: Effects Of Sp On Stimulated Pancreatic Secretionmentioning

confidence: 97%

Effects of Substance P on Pancreatic Exocrine Secretion Stimulated by Secretin, Dopamine and Cholecystokinin in Dogs

Iwatsuki

Yamagishi

Chiba

1986

Clin Exp Pharma Physio

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The effect of substance P (SP) on pancreatic exocrine responses to exogenous cholecystokinin, secretin, and dopamine, were studied in the isolated and blood-perfused pancreas of dogs. Intra-arterial injection of SP had a significant biphasic effect on pancreatic secretion: an initial transient inhibition, followed by an increase in the secretion stimulated by the infusion of cholecystokinin. However, SP caused only an inhibition of secretion stimulated by the infusion of secretin and dopamine. SP increased protein concentration but not bicarbonate concentration in juice stimulated by cholecystokinin, but SP did not affect significantly either protein or bicarbonate concentrations in juice stimulated by secretin and dopamine. These results suggest that SP has greater effects on the pancreatic secretion stimulated by cholecystokinin than that stimulated by secretin and dopamine.

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“…We developed the dog isolated pancreas preparation in situ which was perfused with the animal's own blood (Hashimoto et a/. 1971;Iwatsuki et al 1982). This preparation is convenient for studies of the direct effects of drugs whilst eliminating circulatory, hormonal and central nervous effects on secretion.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Ouabain and Acetazolamide on Secretin‐stimulated Pancreatic Exocrine Secretion in Anaesthetized Dog

Iwatsuki

Horiuchi

Yonekura

et al. 1989

Clin Exp Pharma Physio

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1. The effects of ouabain and acetazolamide on the secretion of pancreatic juice stimulated by secretin in anaesthetized dogs were investigated. 2. Intra-arterial injection of ouabain (1-10 micrograms) and acetazolamide (1-10 mg) caused dose-dependent decreases in the volume of pancreatic juice. When both drugs were added together, the inhibitory effects were significantly higher than for each drug alone. 3. The bicarbonate concentration in the pancreatic juice was decreased and the chloride concentration was increased by ouabain and acetazolamide, but sodium and protein concentrations were not modified. 4. The results suggest that the Na+,K+-ATPase and carbonic anhydrase activities play important roles in water and electrolyte secretion, and that ouabain and acetazolamide inhibit secretin-stimulated pancreatic secretion by acting on different systems in the exocrine cells in dogs.

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Effects of nitroprusside on pancreatic juice secretion in the blood-perfused canine pancreas

Cited by 17 publications

References 17 publications

Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice

Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice

Effects of Substance P on Pancreatic Exocrine Secretion Stimulated by Secretin, Dopamine and Cholecystokinin in Dogs

Inhibitory Effect of Ouabain and Acetazolamide on Secretin‐stimulated Pancreatic Exocrine Secretion in Anaesthetized Dog

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