Effects of nitrogen and phosphorus on arsenite accumulation, oxidation, and toxicity in Chlamydomonas reinhardtii

Wang, Ningxin; Bin, Huang; Xu, Sen; Wei, Zhongbo; Miao, Ai-Jun; Ji, Rong; Yang, Liuyan

doi:10.1016/j.aquatox.2014.10.012

Cited by 50 publications

(24 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and C. antique decreased with an increase of NO 3 -in the cultures, respectively. Wang et al (2014a) observed the same trend in Chlamydomonas reinhardtii and they speculated that more transporters were synthesized to assimilate more As under the N-deficiency condition.…”

Section: Arsenic Uptake Into Algal Cellssupporting

confidence: 59%

“…However, contrasting conclusions were made on the uptake of As(V) and PO 4 3-in some species of microalgae such as Thalassiosira sp., Chaetoceras sp., Synechococcus, S. costatum and Chattonella antique, which were capable of discriminating between the uptake of As(V) and P, because no major change of cellular As levels had been observed when the ratio of P/As in the cultures ranged from 1:4 to 50:1 (Andreae and Klumpp 1979;Budd and Craig 1981;Morris and McCartney 1984;Yamaoka et al 1996). In fact, As(V) uptake may not be only controlled by the PO 4 3-content in culture media, small variations of intracellular PO 4 3-concentrations could also significantly change the toxicity of As(V) in microalgae (Wang et al 2013a(Wang et al , 2014a. Moreover, As speciation may be changed in microalgae under different PO 4 3-conditions.…”

Section: Arsenic Uptake Into Algal Cellsmentioning

confidence: 90%

“…Some microalgae may well be able to survive in aquatic habitats with raised As(V) levels, because they have the ability to rapidly exclude As from the cells (Budd and Craig 1981;Fujiwara et al 2000;Wang et al 2014a). Sanders and Windom (1980) found that although some marine phytoplankton took up As(V) readily, but they incorporated only a small percentage of As(V) into their cells, the remainder was reduced, methylated and then excreted rapidly into the surrounding media.…”

Section: Efflux Of Arsenic Speciesmentioning

confidence: 99%

See 2 more Smart Citations

Review of arsenic speciation, toxicity and metabolism in microalgae

Wang

et al. 2015

Rev Environ Sci Biotechnol

162

100

View full text Add to dashboard Cite

Arsenic is a toxic metalloid and its pollution has become a global environmental problem. This paper reviewed the current knowledge on the speciation, toxicity and metabolism of arsenic in microalgae. A number of arsenic species are present in various microalgae. Due to the great toxicity of inorganic arsenic, microalgae may undergo different processes to reduce the arsenic toxicity, including cell surface binding, arsenite [As(III)] oxidation, arsenate [As(V)] reduction, methylation, transformation into arsenosugars or arsenolipids, chelation of As(III) with glutathione and phytochelatins, as well as excretion from cells. Several genes and enzymes involved in arsenic transformations have been identified and characterized. Many factors, especially nutrient elements (e.g., nitrogen and phosphorus) in cells and in culture, affect arsenic metabolic pathways of microalgae. Arsenic metabolism in the unicellular algae has gained considerable interest because these processes control not only the effectiveness of arsenic phycoremediation, but also the risk of arsenic contamination in algal products. Future research need to focus on (1) the regulative mechanisms of arsenic absorption, biotransformation and excretion at molecular level; (2) the effects of intracellular nutrient dynamics on arsenic speciation; (3) the impacts of culture regime on the arsenic metabolism in microalgae; (4) the transfer of arsenic species across aquatic food web in order to better evaluate the roles of microalgae in arsenic cycling.

show abstract

Section: Arsenic Uptake Into Algal Cellssupporting

confidence: 59%

Section: Arsenic Uptake Into Algal Cellsmentioning

confidence: 90%

Section: Efflux Of Arsenic Speciesmentioning

confidence: 99%

See 1 more Smart Citation

Review of arsenic speciation, toxicity and metabolism in microalgae

Wang

et al. 2015

Rev Environ Sci Biotechnol

162

100

View full text Add to dashboard Cite

show abstract

“…After exposure to As(III), only trace amounts of As(V) and DMA were observed inside the algae, by contrast, other researchers have reported up to 67% oxidation inside microalgae (Wang et al, 2014) and 45% oxidation in cyanobacteria, although the later was measured in growing media (Zhang et al, 2014).…”

Section: Arsenic (Iii) Effects In C Vulgarismentioning

confidence: 64%

“…Recent experiments have demonstrated that at low concentrations of phosphate, different species of microalgae and bacteria are more sensitive to As(V) than As(III), because phosphate reduces some of the toxicity of its 'analogue' arsenate (Karajova et al, 2008;Knauer et al, 1999;Levy et al, 2005;Pawlik-Skowronska et al, 2004;Rubinos et al, 2014;Stolz et al, 2006;Wang et al, 2014) and probably because there is no analogue for As(III) that controls growth rate as phosphate does in the presence of As (V). However, some photosynthetic organisms such as the floating macrophyte Lemna disperm are more sensitive to As(III) than As(V) under low phosphate conditions (Rahman et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The mechanisms of detoxification of As(III), dimethylarsinic acid (DMA) and As(V) in the microalga Chlorella vulgaris

et al. 2016

View full text Add to dashboard Cite

The response of Chlorella vulgaris when challenged by As(III), As(V) and dimethylarsinic acid (DMA) was assessed through experiments on adsorption, efflux and speciation of arsenic (reduction, oxidation, methylation and chelation with glutathione/phytochelatin [GSH/PC]). Our study indicates that at high concentrations of phosphate (1.62 mM of HPO4 2− ), upon exposure to As(V), cells are able to shift towards methylation of As(V) rather than PC formation. Treatment with As(V) caused a moderate decrease in intracellular pH and a strong increase in the concentration of free thiols (GSH). Passive surface adsorption was found to be negligible for living cells exposed to DMA and As(V). However, adsorption of As(III) was observed to be an active process in C. vulgaris, because it did not show saturation at any of the exposure periods. Chelation of As(III) with GS/PC and to a lesser extent hGS/hPC is a major detoxification mechanism employed by C. vulgaris cells when exposed to As(III). The increase of bound As-GS/PC complexes was found to be strongly related to an increase in concentration of As(III) in media. C. vulgaris cells did not produce any As-GS/PC complex when exposed to As(V). This may indicate that a reduction step is needed for As(V) complexation with GSH/PC. C. vulgaris cells formed DMASV -GS upon exposure to DMA independent of the exposure period. As(III) triggers the formation of arsenic complexes with PC and homophytochelatins (hPC) and their compartmentalisation to vacuoles. A conceptual model was devised to explain the mechanisms involving ABCC1/2 transport. The potential of C. vulgaris to bio-remediate arsenic from water appeared to be highly selective and effective without the potential hazard of reducing As(V) to As(III), which is more toxic to humans.

show abstract

Evaluation of green microalgal strains for simultaneous municipal wastewater treatment and heavy metal bioremediation

Ahiahonu,

Anku,

Roopnarain

et al. 2024

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUNDMicroalga‐assisted wastewater treatment systems have gained attention for their efficiency in removing nutrients, chemical oxygen demand, toxic heavy metals and other dissolved compounds, while also producing valuable biomass and demonstrating high CO2 biofixation potential. The current research focuses on investigating the municipal wastewater phycoremediation and heavy metal biosorption ability of three indigenous freshwater microalgal strains: Tetradesmus reginae, Tetradesmus obliquus and Chlorella sorokiniana.RESULTSThe research findings indicate that the microalgal strains T. reginae, T. obliquus and C. sorokiniana exhibited notable performance in biomass accumulation. Specifically, the biomass accumulations were 2.215 ± 0.002, 1.143 ± 0.006 and 0.856 ± 0.021 g L−1, respectively, with initial culture biomasses ranging from 0.5 to 0.6 g L−1. These strains significantly reduced toxic heavy metals (As, 46.86–60.12%; Cd, 52.96–83.18%; Cr, 73.49–82.18%; and Pb, 95.38–96.25%), nutrients (NH4+ & NO3− 100% and PO43− 78–86.41%) as well as chemical oxygen demand (46.02–67.35%), and biosequestered CO2 (0.8–0.18 gCO2 L−1 d−1) during the growth period. Among the strains, T. reginae emerged as the top performer. The Fourier transform infrared spectra of the strain's biomass confirmed the presence of specific functional groups, such as CH, NH, OH and CN, which play a crucial role in ionising and reacting with toxic metal ions and protons in the wastewater.CONCLUSIONThis study has shown that the isolated wild microalgal strains have promising phycoremediation and heavy metal adsorption characteristics. Moreover, they exhibit promising rates of CO2 biosequestration. These findings underscore the potential of microalga‐assisted wastewater treatment systems for efficient and eco‐friendly wastewater remediation, as well as biomass generation and CO2 mitigation. © 2024 Society of Chemical Industry (SCI).

show abstract

Effects of nitrogen and phosphorus on arsenite accumulation, oxidation, and toxicity in Chlamydomonas reinhardtii

Cited by 50 publications

References 37 publications

Review of arsenic speciation, toxicity and metabolism in microalgae

Review of arsenic speciation, toxicity and metabolism in microalgae

The mechanisms of detoxification of As(III), dimethylarsinic acid (DMA) and As(V) in the microalga Chlorella vulgaris

Evaluation of green microalgal strains for simultaneous municipal wastewater treatment and heavy metal bioremediation

Contact Info

Product

Resources

About