Effects of neutron irradiation on ophthalmic fundus structure, visual function and the mechanisms underlying these effects in rats

Mass spectrometry‐based retina proteomics

“…Isotope‐coded affinity tags (ICAT; Gygi et al, 1999) were used first to label peptides differentially for quantitative retina proteomic analyses by LC‐ESI‐MS/MS (Allison et al, 2006). However, the subsequently introduced derivatization of primary amino groups in intact proteins using isobaric tag for relative and absolute quantitation (iTRAQ; Ross et al, 2004) became the popular approach for quantitative proteomics among the stable‐isotope labeling methods in retina proteomics (Barathi et al, 2014; Carmy‐Bennun et al, 2021; Danda et al, 2016; Feng et al, 2021; Hollander et al, 2012; Len et al, 2012; Iqbal et al, 2019; Santos et al, 2018; Yuan et al, 2010). The related tandem mass tag (TMT, Thompson et al, 2003) labeling method has also been applied for this purpose (Deng et al, 2019; D. T. Liu et al, 2021; Mirzaei et al, 2017; VanGuilder et al, 2011; Yan et al, 2021), while one recent study to identify myopia‐regulated proteins in the retinae of myopic chicks (Yu et al, 2020) relied on differential protein tagging of free amino groups with 1‐[ 12 C 6 ]nicotinoyloxy‐succinimide and 1‐[ 13 C 6 ]nicotinoyloxy‐succinimide reagents, respectively, as isotope‐coded protein label (ICPL) (Schmidt et al, 2005).…”

“…Isotope-coded affinity tags (ICAT; Gygi et al, 1999) were used first to label peptides differentially for quantitative retina proteomic analyses by LC-ESI-MS/MS (Allison et al, 2006). However, the subsequently introduced derivatization of primary amino groups in intact proteins using isobaric tag for relative and absolute quantitation (iTRAQ; Ross et al, 2004) became the popular approach for quantitative proteomics among the stable-isotope labeling methods in retina proteomics (Barathi et al, 2014;Carmy-Bennun et al, 2021;Danda et al, 2016;Feng et al, 2021;Hollander et al, 2012;Len et al, 2012;Iqbal et al, 2019;Santos et al, 2018;Yuan et al, 2010). The related tandem mass tag (TMT, Thompson et al, 2003) labeling method has also been applied for this purpose (Deng et al, 2019;D.…”