Effects of Neurotransplantation of Cultured Human Neural and Mesenchymal Stem Cells on Learning and the State of the Brain in Rats after Hypoxia

Loseva, E. V.; Podgornyi, O. V.; Полтавцева, Р. А.; Marei, M. V.; Loginova, N. A.; Kurskaya, O. V.; Сухих, Г. Т.; Chailakhyan, R. K.; Александрова, М. А.

doi:10.1007/s11055-012-9588-9

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…This suggests that neuronal differentiation underlies the ameliorative effects observed in this study. Similarly, Cowley and colleagues showed that neurons could promote the repair of SCI 39 , and others have shown that NSCs can differentiate into neurons and resist cell death 40 . Zhang and Figure 7.…”

Section: Discussionmentioning

confidence: 98%

Neural Stem Cell Transplantation Improves Locomotor Function in Spinal Cord Transection Rats Associated with Nerve Regeneration and IGF-1 R Expression

Zhao

Liu

et al. 2019

Cell Transplant

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Transplantation of neural stem cells (NSCs) is a potential strategy for the treatment of spinal cord transection (SCT). Here we investigated whether transplanted NSCs would improve motor function of rats with SCT and explored the underlying mechanism. First, the rats were divided into sham, SCT, and NSC groups. Rats in the SCT and NSC groups were all subjected to SCT in T10, and were administered with media and NSC transplantation into the lesion site, respectively. Immunohistochemistry was used to label Nestin-, TUNEL-, and NeuN-positive cells and reveal the expression and location of type I insulin-like growth factor receptor (IGF-1 R). Locomotor function of hind limbs was assessed by Basso, Beattie, Bresnahan (BBB) score and inclined plane test. The conduction velocity and amplitude of spinal nerve fibers were measured by electrophysiology and the anatomical changes were measured using magnetic resonance imaging. Moreover, expression of IGF-1 R was determined by real-time polymerase chain reaction and Western blotting. The results showed that NSCs could survive and differentiate into neurons in vitro and in vivo. SCT-induced deficits were reduced by NSC transplantation, including increase in NeuN-positive cells and decrease in apoptotic cells. Moreover, neurophysiological profiles indicated that the latent period was decreased and the peak-to-peak amplitude of spinal nerve fibers conduction was increased in transplanted rats, while morphological measures indicated that fractional anisotropy and the number of nerve fibers in the site of spinal cord injury were increased after NSC transplantation. In addition, mRNA and protein level of IGF-1 R were increased in the rostral segment in the NSC group, especially in neurons. Therefore, we concluded that NSC transplantation promotes motor function improvement of SCT, which might be associated with activated IGF-1 R, especially in the rostral site. All of the above suggests that this approach has potential for clinical treatment of spinal cord injury.

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Section: Discussionmentioning

confidence: 98%

Neural Stem Cell Transplantation Improves Locomotor Function in Spinal Cord Transection Rats Associated with Nerve Regeneration and IGF-1 R Expression

Zhao

Liu

et al. 2019

Cell Transplant

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“…Along with papers providing experiential evidence of the negative outcomes of cell therapy, there is a study that reports an insignificant or even unobservable effect after cell therapy [ 116 ]. It is likely that the effect is short-term as previously described for stem cells evaluated in other experimental models [ 108 , 117 ]. These issues need to be explored in future.…”

Section: Adverse Effects Associated With Cell Therapymentioning

confidence: 95%

Injured Nerve Regeneration using Cell-Based Therapies: Current Challenges

2015

Acta Naturae

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This paper reviews the recent research progress in the past several years on promoting peripheral nerve recovery using stem and progenitory cells. The emphasis is placed on studies aimed at assessing various stem cells capable of expressing neurotrophic and growth factors and surviving after implantation in the nerve or a conduit. Approaches to improving nerve conduit design are summarized. The contribution of stem cells to axonal regeneration and neural repair is discussed. The side effects associated with cell-based treatment are highlighted. From the studies reviewed, it is concluded that the fate of transplanted stem cells needs further elucidation in a microenvironment-dependent manner.

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“…Some authors have detected pre-labeled MSCs even a few months after transplantation (Evaristo-Mendonca et al, 2018). However, other authors note that MSCs survive about 10 days after transplantation into the brain, and by 20 days they have completely degenerated (Loseva et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Allogeneic bone marrow mesenchymal stem cells in the epineurium and perineurium of the recipient rat

Петрова

Исаева

Kolos

et al. 2018

BioComm

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Effects of Neurotransplantation of Cultured Human Neural and Mesenchymal Stem Cells on Learning and the State of the Brain in Rats after Hypoxia

Cited by 3 publications

References 30 publications

Neural Stem Cell Transplantation Improves Locomotor Function in Spinal Cord Transection Rats Associated with Nerve Regeneration and IGF-1 R Expression

Neural Stem Cell Transplantation Improves Locomotor Function in Spinal Cord Transection Rats Associated with Nerve Regeneration and IGF-1 R Expression

Injured Nerve Regeneration using Cell-Based Therapies: Current Challenges

Allogeneic bone marrow mesenchymal stem cells in the epineurium and perineurium of the recipient rat

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