Effects of Neuromedin B on Insulin and Glucagon Release from the Isolated Perfused Rat Pancreas.

Kawai, Koichi; Mukai, Hidehito; Chiba, Yukinobu; Ohmori, Hitoshi; Suzuki, Seiji; Munekata, Eisuke; Yamashita, Kamejiro

doi:10.1507/endocrj1954.36.587

Cited by 6 publications

(7 citation statements)

References 17 publications

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“…However, we did not find Nmb to be expressed outside the islets as evaluated using Nmb LacZ reporter mice. Second, we used a physiological range of exogenous NMB in the current study (0.1-100nM) whereas the stimulatory effect in rat pancreas was observed with 10mM NMB [21] but not with 10nM-1M NMB 32 Thus, although we cannot rule out species differences in responses to NMB, our studies using mouse and human isolated islets convincingly show that NMB is not an insulinreleasing peptide in both mice and humans. It is therefore evident that the conclusions drawn from obesity GWAS association studies on NMB, as well as on insulin release, should be taken with caution until additional independent studies confirm or refute our findings.…”

Section: Discussionmentioning

confidence: 66%

“…In the same experiments, as a control the Gq-coupled receptor agonist carbachol (Cch) caused the expected significant potentiation of glucose-stimulated insulin secretion (Figure 4C). We finally investigated whether there might be species-specific differences, given that previous studies have shown insulin secretion from NMB perfused canine [22], but no effects in NMB treated rat pancreas 32 . Similar to the data obtained with mouse islets, increasing concentrations of NMB did not significantly affect basal or glucose-stimulated insulin secretion from isolated human islets, while the positive control Cch exhibited a significant stimulatory effect (Figure 4D).…”

Section: Nmb Supplementation Does Not Affect Glucose Metabolism Nor Stimulate Insulin Release From Mouse and Human Isletsmentioning

confidence: 99%

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Neuropeptide Neuromedin B does not alter body weight and glucose homeostasis nor does it act as an insulin-releasing peptide

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Huang

et al. 2021

Preprint

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Neuromedin B (NMB) is a member of the neuromedin family of neuropeptides with a high level of region-specific expression in the brain. Several GWAS studies on non-obese and obese patients suggested that polymorphisms in NMB predispose to obesity by affecting appetite control and feeding preference. Furthermore, several studies proposed that NMB can act as an insulin releasing peptide. Since the functional study has never been done, the in vivo role of NMB as modulator of weight gain or glucose metabolism remains unclear. Here, we generated Nmb conditional mice and nervous system deficient NmB mice. We then performed olfactory and food preference analysis, as well as metabolic analysis under standard and high fat diet. Additionally, in direct islet studies we evaluated the role of NMB on basal and glucose-stimulated insulin secretion in mouse and humans.

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Section: Discussionmentioning

confidence: 66%

Section: Nmb Supplementation Does Not Affect Glucose Metabolism Nor Stimulate Insulin Release From Mouse and Human Isletsmentioning

confidence: 99%

Neuropeptide Neuromedin B does not alter body weight and glucose homeostasis nor does it act as an insulin-releasing peptide

Cikes

Atanes

Huang

et al. 2021

Preprint

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“…4 C). We finally investigated whether there might be species-specific differences, given that previous studies have shown insulin secretion from NMB perfused canine 22 , but no effects in NMB treated rat pancreas 32 . Similar to the data obtained with mouse islets, increasing concentrations of NMB did not significantly affect basal or glucose-stimulated insulin secretion from isolated human islets, while the positive control Cch exhibited a significant stimulatory effect (Fig.…”

Section: Resultsmentioning

confidence: 99%

Neuropeptide Neuromedin B does not alter body weight and glucose homeostasis nor does it act as an insulin-releasing peptide

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Cronin

et al. 2022

Sci Rep

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“…Gastrin-releasing peptide Innervation (98) Murine islets (129) and INS-1 cells (130) Effects on endocrine secretion of rat islets (129) Ghrelin Rat islets (131,132) Rat islets (132) Effects on endocrine secretion of rat islets (133,134) and beta cells (135) Growth hormone-releasing hormone Rat islets (136) Effects on endocrine secretion of rat islets (137) Guanylin Beta cells of rat islets show uroguanylin immunoreactivity (138) Kisspeptin Murine and human islets (139) Effects on endocrine secretion of rat islets (140) Murine and human islets (139) Leptin Rat beta cells (141) Effects on endocrine secretion of rat beta cells (142,143) Luteinising hormone-releasing hormone Rat islets (144) Effects on endocrine secretion of rat islets (145) Melanin-concentrating hormone Expressed in murine and human islets and the betaTC3 cell line (146) MCHR1 is expressed in rat islets (147) and in murine and human islets and the betaTC3 cell line (146) Effects on endocrine secretion of rat islets, (147) regulation of beta cell mass (146) Neuromedin B Effects on endocrine secretion of rat islets (148,149) Neuromedin C Effects on endocrine secretion of rat islets (149) Neuromedin U Rat islets (150) Effects on endocrine secretion of rat islets (150) Neuropeptide Y Rat islets, (151) foetal mice, (152) innervation (98) Y1 receptor in rat islets (153) Effects on endocrine secretion of rat islets, (154) beta cell replication …”

Section: Receptor Localisation Bioactivitymentioning

confidence: 99%

“…Expressed in murine and human islets and the betaTC3 cell line (146) MCHR1 is expressed in rat islets (147) and in murine and human islets and the betaTC3 cell line (146) Effects on endocrine secretion of rat islets, (147) regulation of beta cell mass (146) Neuromedin B Effects on endocrine secretion of rat islets (148,149) Neuromedin C Effects on endocrine secretion of rat islets (149) Neuromedin U Rat islets (150) Effects on endocrine secretion of rat islets (150)…”

Section: Melanin-concentrating Hormonementioning

confidence: 99%

Bioactive peptides, networks and systems biology

Boonen

Creemers

Schoofs³

2009

BioEssays

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Bioactive peptides are a group of diverse intercellular signalling molecules. Almost half a century of research on this topic has resulted in an enormous amount of data. In this essay, a general perspective to interpret all these data will be given. In classical endocrinology, neuropeptides were thought of as simple signalling molecules that each elicit one response. However, the fact that the total bioactive peptide signal is far from simple puts this view under pressure. Cells and tissues express many different bioactive peptides and they are also able to respond to many different bioactive peptides, indicating that multiple receptors and signal transduction pathways are present in a single cell. Therefore, the authors suggest that the bioactive peptide signalling system should be regarded in the context of network and systems biology. Bioactive peptides can best be viewed as an extension of the protein interaction network that allows regulating and fine-tuning the metabolism of the different cells and tissues in the body. The cell thus responds to the 'peptidome' instead of to a single peptide. The intracellular part of this signalling network consists of the various signalling transduction cascades. Recently, new systems biology approaches have emerged for the modelling of cell signalling. The network and systems biology approach is also able to shed new light on the evolution of intercellular signalling.

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Effects of Neuromedin B on Insulin and Glucagon Release from the Isolated Perfused Rat Pancreas.

Abstract: The effect of neuromedin

Cited by 6 publications

References 17 publications

Neuropeptide Neuromedin B does not alter body weight and glucose homeostasis nor does it act as an insulin-releasing peptide

Neuropeptide Neuromedin B does not alter body weight and glucose homeostasis nor does it act as an insulin-releasing peptide

Neuropeptide Neuromedin B does not alter body weight and glucose homeostasis nor does it act as an insulin-releasing peptide

Bioactive peptides, networks and systems biology

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