Effects of Netrin-1 and NHE1 Participation on the Migration of Macrophages Driven by CCL2

Zhang, Shiyue; Mo, Xiangang; Zhang, Shuping; Chen, Juhai; Wang, Lan; Yang, Huifeng

doi:10.14715/cmb/2022.68.6.18

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…In this study, firstly, through cytological experiment, we verified that β2-AR, β2-AR stimulant on the surface of macrophages can not only promote the conversion of macrophages to M2 type but also inhibit the conversion of macrophages to M1 type (24), and confirmed that β-AR blockers with different degrees of blocking effect on β2-AR, including metoprolol, propranolol and ICI118551, are agonists that can regulate the polarization state of macrophages in the opposite way to that of β2-AR stimulant salbutamol through β2-AR (25)(26)(27). The β2-AR stimulant or the β-AR blocker performs mutual reverse intervention of promotion or inhibition on the process that ox-LDL promotes the M2 type differentiation of macrophages, further verifying that ox-LDL can promote the M2 type differentiation of macrophages (18)(19)(20), and further verifies that M2 type macrophages are more sensitive to lipid toxicity than M1-type macrophages (19,28). However, its phagocytic ability is stronger than that of M1-type macrophages (7), and it is more likely to form foam cells (19,29).…”

Section: Discussionmentioning

confidence: 83%

“…However, its phagocytic ability is stronger than that of M1-type macrophages (7), and it is more likely to form foam cells (19,29). M2type macrophages that engulf ox-LDL are more likely to die than other forms of macrophages (19,28). The in-depth study on the mode of death of macrophages after consuming ox-LDL has revealed that, in addition to apoptosis, non-apoptotic modes of death such as pyroptosis, in which the cell membrane is ruptured, are also included.…”

Section: Discussionmentioning

confidence: 99%

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Oxidized low-density lipoprotein induces M2-type differentiation of macrophages to promote the protracted progression of atherosclerotic inflammation in high-fat diet-fed ApoE -/- mice

Bo Zhang,

Weichang Xia,

Jingqun Zhou

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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In this study, the significance of oxidized low-density lipoprotein (ox-LDL) in promoting the progression of atherosclerosis was investigated by inducing the differentiation of macrophages into the M2 subtype within a high-fat diet-induced ApoE -/-mouse model. The study also evaluated the effects of β2-AR agonists and blockers on this process. Ox-LDL was found to have significantly promoted the differentiation of macrophages into the M2 type and induced related functional alterations. Furthermore, it activated the pyroptosis pathway and encouraged the release of lactate dehydrogenase. The administration of β2-AR agonists intensified these processes, while β2-AR blockers had the opposite effect. In animal experiments, the model group displayed elevated numbers of M2-type macrophages beneath the aortic root intima, an increased rate of plaque destruction, and the formation of atherosclerotic plaques compared to the control group. The SAL (Salbutamol) group exhibited even more severe plaque development than the model group. Conversely, the ICI (ICI118551) group demonstrated M2-type macrophage levels comparable to the control group, with a higher plaque destruction rate than controls but significantly lower than the model group, and no atherosclerotic plaques. These findings suggest that ox-LDL promoted the differentiation of recruited monocytes into M2-type macrophages, leading to a shift in the inflammatory response from M1 to M2 macrophages. This alteration resulted in the persistence of atherosclerotic inflammation, as M2-type macrophages were prone to cell membrane rupture (such as pyroptosis), contributing to the continuous recruitment of circulating monocytes and heightened inflammatory reactions within atherosclerotic plaques. Consequently, this process fueled the progression of atherosclerosis.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Oxidized low-density lipoprotein induces M2-type differentiation of macrophages to promote the protracted progression of atherosclerotic inflammation in high-fat diet-fed ApoE -/- mice

Bo Zhang,

Weichang Xia,

Jingqun Zhou

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…Previous studies indicated that the subtype of NHE1 acts an important role in cell migration 15 . Inhibition or knockout of NHE1 can inhibit macrophage and breast cancer cell migration 38,39 . Thus, in order to determine the role of the expression modification of NHE1 in 1,25(OH) 2 D 3 ameliorating cell migration, we employed qRT‐PCR to measure the transcript level of NHE1.…”

Section: Resultsmentioning

confidence: 99%

“…15 Inhibition or knockout of NHE1 can inhibit macrophage and breast cancer cell migration. 38,39 Thus, in order to determine the role of the expression modification of NHE1 in 1,25 (OH) 2 D 3 ameliorating cell migration, we employed qRT-PCR to measure the transcript level of NHE1. As revealed in Figure 4A, the transcript level of NHE1 in LLC cells was predominately down-regulated after the 24 h treatment of 1,25(OH) 2 D 3 (100 nM).…”

Section: 25(oh) 2 D 3 Inhibited Activity and Expression Of Nhe1mentioning

confidence: 99%

1,25(OH)₂D₃ inhibits Lewis lung cancer cell migration via NHE1‐sensitive metabolic reprograming

Chen,

Zhu,

Wang

et al. 2023

IUBMB Life

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High prevalence and metastasis rates are characteristics of lung cancer. Glycolysis provides energy for the development and metastasis of cancer cells. The 1,25‐dihydroxy vitamin D3 (1,25(OH)2D3) has been linked to reducing cancer risk and regulates various physiological functions. We hypothesized that 1,25(OH)2D3 could be associated with the expression and activity of Na+/H+ exchanger isoform 1 (NHE1) of Lewis lung cancer cells, thus regulating glycolysis as well as migration by actin reorganization. Followed by online public data analysis, Vitamin D3 receptor, the receptor of 1,25(OH)2D3 has been proved to be abundant in lung cancers. We demonstrated that 1,25(OH)2D3 treatment suppressed transcript levels, protein levels, and activity of NHE1 in LLC cells. Furthermore, 1,25(OH)2D3 treatment resets the metabolic balance between glycolysis and OXPHOS, mainly including reducing glycolytic enzymes expression and lactate production. In vivo experiments showed the inhibition effects on tumor growth as well. Therefore, we concluded that 1,25(OH)2D3 could amend the NHE1 function, which leads to metabolic reprogramming and cytoskeleton reconstruction, finally inhibits the cell migration.

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Research on the coordinated development of resource-based cities in Sichuan Province: from the perspective of industrial structure and ecological environment

Liu

Fan

Yang

et al. 2023

Front. Environ. Sci.

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During their journey of developing, resource-based cities gradually deplete the resources on which they rely for survival. Scientific and reasonable research on the industrial and ecological aspects of resource-based cities is conducive to the coordinated development of cities. In order to further analyze the industrial structure of resource-based cities systematically and analyze the comprehensive level of resource-based cities from multi-dimensional perspective. This paper took 8 resource-based cities in Sichuan Province as the research object, and constructed the index system from two systems: industrial structure and ecological environment, then the shift-share analysis, entropy weight method and capacity coupling coefficient model were used to analyze their level of industrial structure, ecological environment and the coupling relationship respectively. According to the results of the study, it can be concluded that the main influencing factor in the development of industrial structure is the industrialmix effect, while the ecological level presents a decreasing level due to the lack of control of total industrial solid waste and energy consumption. The coupling degree between industrial structure and ecological environment in resource-based cities in Sichuan Province is relatively stable, and the coupling coordination degree also gradually tends to a stable state. In the subsequent development, the focus should be on the coal mining and dressing industry and the power, heat production and supply industry. Starting with the actual industrial structure of resource-based cities and specific indicators that affected the ecological environment, this paper hereby analyzed the development momentum and unified and coordinated development status of resource-based cities. The main purpose of this paper is providing some technical support for resource-based cities to improve their coordinated urban development, and giving policy suggestions for the coordinated development of resource-based cities.

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Effects of Netrin-1 and NHE1 Participation on the Migration of Macrophages Driven by CCL2

Cited by 3 publications

References 14 publications

Oxidized low-density lipoprotein induces M2-type differentiation of macrophages to promote the protracted progression of atherosclerotic inflammation in high-fat diet-fed ApoE -/- mice

Oxidized low-density lipoprotein induces M2-type differentiation of macrophages to promote the protracted progression of atherosclerotic inflammation in high-fat diet-fed ApoE -/- mice

1,25(OH)₂D₃ inhibits Lewis lung cancer cell migration via NHE1‐sensitive metabolic reprograming

Research on the coordinated development of resource-based cities in Sichuan Province: from the perspective of industrial structure and ecological environment

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Effects of Netrin-1 and NHE1 Participation on the Migration of Macrophages Driven by CCL2

Cited by 3 publications

References 14 publications

Oxidized low-density lipoprotein induces M2-type differentiation of macrophages to promote the protracted progression of atherosclerotic inflammation in high-fat diet-fed ApoE -/- mice

Oxidized low-density lipoprotein induces M2-type differentiation of macrophages to promote the protracted progression of atherosclerotic inflammation in high-fat diet-fed ApoE -/- mice

1,25(OH)2D3 inhibits Lewis lung cancer cell migration via NHE1‐sensitive metabolic reprograming

Research on the coordinated development of resource-based cities in Sichuan Province: from the perspective of industrial structure and ecological environment

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1,25(OH)₂D₃ inhibits Lewis lung cancer cell migration via NHE1‐sensitive metabolic reprograming