Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus

Abstract

Berdún S, Rychter J, Vergara P. Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus.

The evolution of nerve growth factor inhibition in clinical medicine

“…These data suggest that TrkA and its ligand NGF are crucially important for pain perception in adult rats. However, TrkA is not only found on neurons but also exists on a variety of non-neuronal cells including human keratinocytes 30 , synovial fibroblasts 31 , mast cells 32 and all major types of peripheral leukocytes 33 . These data suggest that several distinct non-neuronal mechanisms are linked to NGF.…”

Surgical intestinal manipulation increases gene expression of TrkA, CGRP, and PAR‐2 IN dorsal root ganglia in the rat

“…Although MCs can modulate vagal afferents, mediators released during MC degranulation, such as serotonin and histamine, can also act on splanchnic afferents, particularly in inflammatory conditions . We previously demonstrated that intestinal manipulation activates PMCs, but not mucosal MCs (data not shown), in patients undergoing intestinal surgery and in an experimental rat model of POI . It is interesting that we did not observe differences in number or distribution of CTMC within the intestinal wall.…”

“…26 We previously demonstrated that intestinal manipulation activates PMCs, but not mucosal MCs (data not shown), in patients undergoing intestinal surgery and in an experimental rat model of POI. 10,27 It is interesting that we did not observe differences in number or distribution of CTMC within the intestinal wall. It may be possible that 24-h timing is not enough to elicit changes in that particular population of MCs in contrast to long-term models of inflammation.…”

hTERT Transduction Extends the Lifespan of Primary Pediatric Low-Grade Glioma Cells While Preserving the Biological Response to NGF

“…Samples were separated by SDS-PAGE, transferred to Hybond-P membranes (GE Healthcare, Chicago, IL, Unites States), probed with specific primary and horseradish peroxidase-conjugated secondary Abs, then binding visualized by chemiluminescence (GE Healthcare) with Chemidoc XRS System (Bio-RaD, Hercules, CA, United States). Western Blot analysis of MASH-1 expression was also performed in the presence of K252a, a pan Trk inhibitor (Sigma-Aldrich), [ 37 ]. Densitometry was performed using Imager ChemiDoc XRS System.…”