Effects of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocyte complexes

Transvaginal Follicle Aspiration in Thai Swamp Buffalo Heifers Using Different Vacuum Pressures after FSH Pretreatment (Bubalus bubalis)

“…This is the first time this has been reported in swamp buffalo. It is noted that more bovine oocytes were recovered from slaughterhouse ovaries at the high vacuum pressure, 110 to 130 mmHg [2,3] and this is contrary to our result. In cattle, OPU had been performed in various studies, reviewed by Bols et al [2].…”

“…Thus there is the possibility that the highest recovery rate for oocytes in the 60 mmHg group may be strongly affected by the FSH pretreatment, making it difficult to conclude that oocyte recovery rate is improved by the lower vacuum pressure. The proportion of good oocytes surrounded with a cumulus mass decreased progressively when the vacuum pressure increased [2,3]. In the study the number of good oocytes with a cumulus mass did not improve as a result of the vacuum pressures used.…”

“…Our earlier report [12] showed that it was possible to collect oocytes from swamp buffalo with a recovery rate of 63%, however, a large number of denuded oocytes and free zona, without cytoplasm, were found more than 50% of the recovered eggs. In cattle, it was shown that the vacuum pressure of OPU could play a role on both the recovery rate and the oocyte quality [2,3], although no studies in swamp buffalo were performed. The objective of this experiment was to study oocyte recovery in swamp buffalo after FSH pretreatment and using different vacuum pressures.…”

Caracterização de seqüelas subseqüentes à punção folicular em bovinos

“…São relatadas a utilização de agulhas com calibres 17 (Gibbons et al 1994, Fry et al 1998, Schernthaner et al 1999, 18 (Stubbings & Walton 1995, Boni et al 1997), 19 (Machatková et al 2000 e 20 (Bols et al 1996b) Gauge, descartáveis (Machatková et al 2000) ou não (Vos et al 1994, Stubbings & Walton 1995, Boni et al 1997. Agulhas de menor calibre apresentam menor área de corte, sendo potencialmente menos danosas ao tecido, enquanto agulhas descartáveis, por terem o bisel mais afiado, exercem menor pressão sobre as estruturas puncionadas (Bols et al 1997).…”

Técnica de fatiamento do ovário para obtenção de oócitos em cutias (Dasyprocta prymnolopha)

“…Várias técnicas para obtenção de CCOs podem ser utilizadas e possuem impacto considerável sobre a quantidade e a morfologia dos CCOs recuperados e, consequentemente, sobre a competência no desenvolvimento oócitário (Bols et al 1997). Os CCOs podem ser obtidos da tuba uterina, logo após a ovulação, sendo que neste caso, o processo de maturação oocitária já se iniciou in vivo, ou de folículos pré-ovulatórios, imaturos e por vezes atrésicos, havendo necessidade de ser realizada a maturação oocitária in vitro (Wani 2002).…”