Transvaginal Follicle Aspiration in Thai Swamp Buffalo Heifers Using Different Vacuum Pressures after FSH Pretreatment (Bubalus bubalis)

Abstract

ABSTRACT. The objective of the experiment was to study oocyte recovery by transvaginal, ultrasound-guided, follicle aspiration, from Thai swamp buffalo using different vacuum pressures. Six adult buffalo heifers, aged 2.5-3.0 yrs were treated with a total dose of 280 mg FSH, given twice a day in a divided doses over a three day period (60/60 mg, 50/50 mg, 30/30 mg) at d7 after progesterone implant. Three vacuum pressures were used; 100 (n=12), 80 (n=12) and 60 mmHg (n=12) and all of the pressures were performed in each anima l. The animals were treated repeatedly and collection took place using 2 sets of each pressure every 2 months, giving a total of 36 collections from each animal. The oocyte recovery rates from each pressure were 81.2% (69/85) 79.1% (53/67) and 90.3% (93/103) for 100, 80 and 60 mmHg respectively. The number of oocytes collected per donor were 5.33 ± 3.27, 4.42 ± 2.71 and 7.75 ± 4.31 respectively. The quality of the oocytes did not improved with the lower vacuum pressure. In conclusion, the application of FSH pret reatment improves the yield of oocytes from Thai, swamp buffalo heifers after gonadotropin treatment when using the vacuum pressures betw een 60-100 mmHg. KEY WORDS: OPU, swamp buffalo, vacuum pressures.J. Vet. Med. Sci. 66(8): 973-975, 2004 In swamp buffalo, the reproductive technology has not been studied as extensively as those of cattle and river buffalo. The limiting factors are poor in vivo embryo production [15] and a very low recovery rate of immature oocytes from slaughterhouse ovaries [1,14] which make it difficult to apply the technology in this species. Transvaginal, ultrasound guided, oocyte retrieval (Ovum Pick Up, OPU) is a non invasive procedure for collecting oocytes from live animals. This technique, together with in vitro fertilization, could be the way to produce a large number of superior animals [5,9]. Our earlier report [12] showed that it was possible to collect oocytes from swamp buffalo with a recovery rate of 63%, however, a large number of denuded oocytes and free zona, without cytoplasm, were found more than 50% of the recovered eggs. In cattle, it was shown that the vacuum pressure of OPU could play a role on both the recovery rate and the oocyte quality [2,3], although no studies in swamp buffalo were performed. The objective of this experiment was to study oocyte recovery in swamp buffalo after FSH pretreatment and using different vacuum pressures.Follicle aspiration by OPU was performed in 6 Thai swamp buffalo heifers (2.5-3.0 yrs old). The program of superstimulation by gonadotropin was a modification of our previous work in prepubertal buffaloes [16]. The animals were stimulated with a total dose of 280 mg Follicle Stimulating Hormone (FSH, Folltropin ® , Vetaphaarm, Australia) after a progesterone implant (Crestar ® , Intervet, The Netherlands). The treatment was started on d7 after the implant. A total of 6 injections was given twice a day (60/60 mg, 50/50 mg, 30/30 mg), intramuscularly, over a period of three days. An intramuscular injection o...

Ovum Pick‐up in Cycling and Lactating Postpartum Swamp Buffaloes (Bubalis bubalis)

“…This contradicts our finding in previous studies, in pre‐puberal buffaloes treated with FSH and heifers treated with PMSG, where only low numbers of good quality oocytes were recovered (Promdireg et al. 2000; Techakumphu et al. 2004a,b).…”

“…In agreement with previous studies in heifers and pre‐pubertal buffalo calves (Promdireg et al. 2000; Techakumphu et al. 2000a,b, 2004a,b; ), the administration of FSH before OPU can improve the yield of oocytes.…”

“…FSH treatment increased the number of aspirated follicles and oocytes in both reproductive status, confirming most previous studies in buffaloes (Boni 1994; Boni et al. 1997; Techakumphu et al. 2000a,b, 2004a,b; ) and in cattle (reviewed by Faber et al.…”

“…Before OPU, the buffaloes were restrained in a squeeze chute and tranquilized with 1 ml of Xylacine HCL (Rompun ® , Bayer Korea Ltd. Seoul, Korea), followed 10 min later, by 2 ml 2% lidocaine hydrochoride, given epidurally (OLIC, Sweden). OPU was performed as described in our previous study (Techakumphu et al. 2004a,b).…”

Efeito do tratamento com somatotrofina bovina recombinante (bST) na população folicular e na produção in vitro de embriões bubalinos

“…Em fêmeas bubalinas (Swamp buffaloes) também foi relatada a influência da utilização de diferentes pressões de vácuo (60, 80 e 100 mmHg) em programas de aspiração folicular (THECHAKUMPHU et al, 2004). Neste estudo os autores utilizaram novilhas superestimuladas e, para a aspiração folicular, agulha longa (34 (HASHIMOTO et al, 1999).…”