Effects of Nanoparticle Size on Cellular Uptake and Liver MRI with Polyvinylpyrrolidone-Coated Iron Oxide Nanoparticles

Huang, Jing; Bu, Lihong; Xie, Jin; Chen, Kai; Cheng, Zhen; Li, Xingguo; Chen, Xiaoyuan

doi:10.1021/nn101643u

Cited by 428 publications

(335 citation statements)

References 54 publications

(158 reference statements)

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“…When the hepatic carcinoma reached 3-5 mm in diameter, we intravenously injected Octapod-30 and Spherical-16 into the nude mice (2 mg Fe per kg) and scanned the animals at a 7T microMRI scanner. As hepatic tumours contain much less active Kupffer cells and macrophages 36 , they do not accumulate iron oxide nanoparticles as efficiently as normal liver tissues do 40,41 . Thus, the hepatic tumours would show pseudo-positive contrast as compared with normal liver tissues.…”

Section: Synthesis and Characterizationmentioning

confidence: 99%

Octapod iron oxide nanoparticles as high-performance T2 contrast agents for magnetic resonance imaging

et al. 2013

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Spherical superparamagnetic iron oxide nanoparticles have been developed as T 2 -negative contrast agents for magnetic resonance imaging in clinical use because of their biocompatibility and ease of synthesis; however, they exhibit relatively low transverse relaxivity. Here we report a new strategy to achieve high transverse relaxivity by controlling the morphology of iron oxide nanoparticles. We successfully fabricate size-controllable octapod iron oxide nanoparticles by introducing chloride anions. The octapod iron oxide nanoparticles (edge length of 30 nm) exhibit an ultrahigh transverse relaxivity value (679.3±30 mM À 1 s À 1 ), indicating that these octapod iron oxide nanoparticles are much more effective T 2 contrast agents for in vivo imaging and small tumour detection in comparison with conventional iron oxide nanoparticles, which holds great promise for highly sensitive, early stage and accurate detection of cancer in the clinic.

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Section: Synthesis and Characterizationmentioning

confidence: 99%

Octapod iron oxide nanoparticles as high-performance T2 contrast agents for magnetic resonance imaging

et al. 2013

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“…Of these characteristics, the particle size is a crucial factor which determines NP endocytosis pathway [17,18], uptake rate and efficiency [6,16,[19][20][21][22], in vitro cytotoxicity [23][24][25][26][27][28], immune responses [27][28][29][30], and the final localisation [15,31,32]. Thus size-dependent uptake of various NPs has been intensively investigated and widely reported.…”

Section: Introductionmentioning

confidence: 99%

“…Some have used the mass concentration while others used the particle number concentration. The third one is the delivery efficiency, often expressed as the NP number [6,16,43,51], the mass amount (pg/cell) [16,41], or the relative amount of labelled fluorescence dye [48,52] taken up by each cell. In addition, the difference in the shape and surface property from a variety of materials and surface modified functional groups may also cause the inconsistency.…”

Section: Introductionmentioning

confidence: 99%

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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a b s t r a c tIt is well known that delivery efficiency to cells is highly dependent on particle size and the administered dose. However, there is a marked discrepancy in many reports, mainly due to the inconsistency in assessment of various parameters. In this particular research, we designed experiments using layered double hydroxide nanoparticles (LDH NPs) to specifically elucidate the effect of particle size, dose and dye loading manner on cellular uptake. Using the number of LDH NPs taken up by HCT-116 cells as the indicator of delivery efficiency, we found that (1) the size of sheet-like LDH in the range of 40-100 nm did not significantly affect their cellular uptake; (2) cellular uptake of 40 and 100 nm LDH NPs was increased proportionally to the number concentration below a critical value, but remained relatively constant beyond the critical value; and (3) the effect of the dye loading manner is mainly dependent on the loading capacity or yield. In particular, the loading capacity is determined by the NP specific surface area. This research may be extended to a larger size range to examine the size effect, but suggests that it is necessary to set up a protocol to evaluate the effects of NP's physicochemical properties on the cellular delivery efficiency.

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“…38,39 For in vivo tumor imaging, M-HFn nanoparticles can be accumulated in tumor tissues via receptor-mediated pathway and not be cleared quickly. 11 It was also found by acute toxic study on mice that there was no lethal effect with injection of the M-HFn nanoparticles at proposed high dose over 14 days.…”

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confidence: 99%

Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size

Cai¹,

Cao²,

He³

et al. 2015

IJN

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Purpose This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells. Materials and methods M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7–5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco’s Modified Eagle’s Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections. Results The saturation magnetization ( M s ), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r 2 of 321 mM −1 s −1 , allowing to detect MDA-MB-231 breast cancer cells as low as 10 4 cells mL −1 . Conclusion The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

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Effects of Nanoparticle Size on Cellular Uptake and Liver MRI with Polyvinylpyrrolidone-Coated Iron Oxide Nanoparticles

Cited by 428 publications

References 54 publications

Octapod iron oxide nanoparticles as high-performance T2 contrast agents for magnetic resonance imaging

Octapod iron oxide nanoparticles as high-performance T2 contrast agents for magnetic resonance imaging

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size

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