Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

Auvinen, Vili-Veli; Merivaara, Arto; Kiiskinen, Jasmi; Paukkonen, Heli; Laurén, Patrick; Hakkarainen, Tiina; Koivuniemi, Raili; Sarkanen, Riina; Ylikomi, Timo; Laaksonen, Timo

doi:10.1016/j.cryobiol.2019.09.005

Cited by 13 publications

(5 citation statements)

References 61 publications

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“…Furthermore, especially when designing freeze-drying protocol suitable for cells and biologics the critical quality attributes are not limited to the outcome but also the optimization of the environment surrounding them during the process. The importance of understanding these aspects was noticed in another study of ours where NFC hydrogel was utilized in the freeze-drying of hepatocellular carcinoma 3D cell spheroids . Due to the lack of full awareness of the effects of the interactions between the cells, their extracellular matrix and lyoprotectants during freeze-drying, as well as the absence of the comprehensive quality attributes for the FD sample, there were challenges in preserving the functionality of these 3D cell spheroids.…”

Section: Introductionmentioning

confidence: 99%

“…The importance of understanding these aspects was noticed in another study of ours where NFC hydrogel was utilized in the freeze-drying of hepatocellular carcinoma 3D cell spheroids. 16 Due to the lack of full awareness of the effects of the interactions between the cells, their extracellular matrix and lyoprotectants during freezedrying, as well as the absence of the comprehensive quality attributes for the FD sample, there were challenges in preserving the functionality of these 3D cell spheroids.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Insights on Successful Reconstitution of Freeze-Dried Nanofibrillated Cellulose Hydrogel

Koivunotko

Merivaara

Niemelä

et al. 2021

ACS Appl. Bio Mater.

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The diversity and safety of nanofibrillated cellulose (NFC) hydrogels have gained a vast amount of interest at the pharmaceutical site in recent years. Moreover, this biomaterial has a high potential to be utilized as a protective matrix during the freeze-drying of heat-sensitive pharmaceuticals and biologics to increase their properties for long-term storing at room temperature and transportation. Since freeze-drying and subsequent reconstitution have not been optimized for this biomaterial, we must find a wider understanding of the process itself as well as the molecular level interactions between the NFC hydrogel and the most suitable lyoprotectants. Herein we optimized the reconstitution of the freeze-dried NFC hydrogel by considering critical quality attributes required to ensure the success of the process and gained insights of the obtained experimental data by simulating the effects of the used lyoprotectants on water and NFC. We discovered the correlation between the measured characteristics and molecular dynamics simulations and obtained successful freeze-drying and subsequent reconstitution of NFC hydrogel with the presence of 300 mM of sucrose. These findings demonstrated the possibility of using the simulations together with the experimental measurements to obtain a more comprehensive way to design a successful freeze-drying process, which could be utilized in future pharmaceutical applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Insights on Successful Reconstitution of Freeze-Dried Nanofibrillated Cellulose Hydrogel

Koivunotko

Merivaara

Niemelä

et al. 2021

ACS Appl. Bio Mater.

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“…It is cited in the majority of patents as a versatile material, due to the presence of a network of interconnected pores allowing the retention of a large number of compounds ranging from drugs to cosmetics for skin treatment. For example, WO 2016/193548 A1 [ 88 ] relates to a process for producing sterile nanofibrillar cellulose hydrogel for use in various applications; WO 2018/108341 A1 [ 89 ] describes a method for freeze-drying cells in a nanofibrillar cellulose hydrogel; WO 2018/109281 A1 [ 90 ] refers to a method for drying nanofibrillar cellulose hydrogel; and WO 2016/102766 A1 [ 91 ] discusses the treatment of nanofibrillar cellulose hydrogel at elevated temperatures. Although the documents give the intended use of the hydrogel, the cited patents were classified accordingly into suitable clusters regarding the field of the invention.…”

Section: Resultsmentioning

confidence: 99%

Nanocellulose, the Green Biopolymer Trending in Pharmaceuticals: A Patent Review

Garcia,

Beck,

Brandalise

et al. 2024

Pharmaceutics

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The use of nanocellulose in pharmaceutics is a trend that has emerged in recent years. Its inherently good mechanical properties, compared to different materials, such as its high tensile strength, high elastic modulus and high porosity, as well as its renewability and biodegradability are driving nanocellulose’s industrial use and innovations. In this sense, this study aims to conduct a search of patents from 2011 to 2023, involving applications of nanocellulose in pharmaceuticals. A patent search was carried out, employing three different patent databases: Patentscope from World Intellectual Property Organization (WIPO); Espacenet; and LENS.ORG. Patents were separated into two main groups, (i) nanocellulose (NC) comprising all its variations and (ii) bacterial nanocellulose (BNC), and classified into five major areas, according to their application. A total of 215 documents was retrieved, of which 179 were referred to the NC group and 36 to the BNC group. The NC group depicted 49.7%, 15.6%, 16.2%, 8.9% and 9.5% of patents as belonging to design and manufacturing, cell culture systems, drug delivery, wound healing and tissue engineering clusters, respectively. The BNC group classified 44.5% of patents as design and manufacturing and 30.6% as drug delivery, as well as 5.6% and 19.4% of patents as wound healing and tissue engineering, respectively. In conclusion, this work compiled and classified patents addressing exclusively the use of nanocellulose in pharmaceuticals, providing information on its current status and trending advancements, considering environmental responsibility and sustainability in materials and products development for a greener upcoming future.

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“…By reducing AuNPs in situ in the presence of bacterial cellulose (BC) fibers, AuNP-bacterial cellulose hydrogels were created. The SERS signal was significantly enhanced due to the spatial deformation of the hydrogels drying process [ 195 , 196 ]. A microstructure of uniform multilayers cellulose compounds characterized the hydrogel.…”

Section: Applications Of Cellulosementioning

confidence: 99%

A Review on the Modification of Cellulose and Its Applications

et al. 2022

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The latest advancements in cellulose and its derivatives are the subject of this study. We summarize the characteristics, modifications, applications, and properties of cellulose. Here, we discuss new breakthroughs in modified cellulose that allow for enhanced control. In addition to standard approaches, improvements in different techniques employed for cellulose and its derivatives are the subject of this review. The various strategies for synthetic polymers are also discussed. The recent advancements in polymer production allow for more precise control, and make it possible to make functional celluloses with better physical qualities. For sustainability and environmental preservation, the development of cellulose green processing is the most abundant renewable substance in nature. The discovery of cellulose disintegration opens up new possibilities for sustainable techniques. Based on the review of recent scientific literature, we believe that additional chemical units of cellulose solubility should be used. This evaluation will evaluate the sustainability of biomass and processing the greenness for the long term. It appears not only crucial to dissolution, but also to the greenness of any process.

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Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

Cited by 13 publications

References 61 publications

Molecular Insights on Successful Reconstitution of Freeze-Dried Nanofibrillated Cellulose Hydrogel

Molecular Insights on Successful Reconstitution of Freeze-Dried Nanofibrillated Cellulose Hydrogel

Nanocellulose, the Green Biopolymer Trending in Pharmaceuticals: A Patent Review

A Review on the Modification of Cellulose and Its Applications

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