Effects of nano-surface properties on initial osteoblast adhesion and Ca/P adsorption ability for titanium alloys

Abstract

Titanium alloys (Ti6Al4V), while subjected to high temperature surface treatment, experience altered nano-surface characteristics. The effects of such surface treatments are examined, including the initial adhesion force experienced by osteoblasts, the Ca/P adsorption capability, and the nano-surface properties, including the amounts of amphoteric Ti-OH groups, surface topography, and surface roughness. The initial adhesion force is considered a quantitative indicator of cyto-compatibility in vitro. Previously, a cyto-detacher was applied in a pioneer attempt measuring the initial adhesion force of fibroblasts on a metal surface. Presently, the cyto-detacher is further applied to evaluate the initial adhesion force of osteoblasts. Results reveal that (1) titanium alloys subjected to heat treatment could promote the adsorption capability of Ca and P; (2) titanium alloys subjected to heat treatment could have higher initial osteoblast adhesion forces; (3) the adhesion strength of osteoblasts, ranging from 38.5 to 58.9 nN (nanonewtons), appears stronger for rougher surfaces. It is concluded that the heat treatment could have impacted the biocompatibility in terms of the initial osteoblast adhesion force and Ca/P adsorption capability.

Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration—In vitrostudy

“…Platelet adhesion and activation have been found to increase on textured surfaces,15 which is expected to enhance endosseous integration due to the formation of an osteoconductive/osteoinductive blood thrombus that is stabilized on the implant surface via mechanical interlocking of fibrin fibers 4, 21, 32, 44. Finally, micro/nanoscale surface features have also been shown to facilitate the precipitation of bioactive minerals (e.g., calcium phosphates) from the plasma, by serving as nucleation sites, which results in the subsequent formation of an apatite layer on the surface that could lead to enhanced osseointegration 16…”

“…4,21,32,44 Finally, micro/nanoscale surface features have also been shown to facilitate the precipitation of bioactive minerals (e.g., calcium phosphates) from the plasma, by serving as nucleation sites, which results in the subsequent formation of an apatite layer on the surface that could lead to enhanced osseointegration. 16…”

“…Besides the ability to self‐passivate, which protects the metal from corrosion and increases its bioactivity, three decades of surface engineering on titanium implants have resulted in the development of numerous surface modification technologies to improve the degree of tissue integration 15–20. Of these, techniques that aim at introducing a surface micro/nanotopography have been consistently demonstrated to have a positive effect in this process 9, 21–31.…”

“…[9][10][11][12][13][14] Besides the ability to self-passivate, which protects the metal from corrosion and increases its bioactivity, three decades of surface engineering on titanium implants have resulted in the development of numerous surface modification technologies to improve the degree of tissue integration. [15][16][17][18][19][20] Of these, techniques that aim at introducing a surface micro/nanotopography have been consistently demonstrated to have a positive effect in this process. 9,[21][22][23][24][25][26][27][28][29][30][31] This knowledge, however, cannot be directly translated into the modification of ceramic implants, as most of these techniques could cause surface defects and mechanical failure, or could result in leaching of stabilizers (e.g., yttrium), aging, and ultimately implant failure.…”

Characteristics and cyto-compatibility of Collagen/Ca–P coatings on Ti6Al4V substrate

“…The specimens were removed from the SBF and rinsed with double-distilled water to remove loose deposits. The samples were then dried in air and stored in desiccators prior to surface examination [17].…”