Effects of nano-curcumin on gene expression of insulin and insulin receptor

Gouda, Weaam; Hafiz, Naglaa A.; Mageed, Lamiaa; Alazzouni, Ahmed S.; Khalil, Wagdy K. B.; Afify, Mie; Abdelmaksoud, Mohamed D. E.

doi:10.1186/s42269-019-0164-0

Cited by 26 publications

(7 citation statements)

References 34 publications

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“…45 TEM microscopy and FT-IR spectroscopy proved the successful formulation of curcumin in the nano range while preserving the chemical active entities of curcumin. 62 Whereas the zeta potential analysis revealed the acceptable charge that denotes the stability of the dispersed formula, in agreement with previous studies. 63,64 Clomiphene, as the standard drug of choice for PCOS, reinstated the sex hormone levels.…”

supporting

confidence: 90%

Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Abuelezz¹,

Shabana²,

Rashed³

et al. 2021

JEP

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Purpose Polycystic ovary syndrome (PCOS) is a prevalent female endocrine disorder. 50–70% of PCOS patients suffer from glucose intolerance, insulin and β cell impairments. Updated studies reveal the crucial regulatory role of inflammation modulators in various diseases, by manipulating autophagy and oxidative stress. However, the data available about autophagy in PCOS pancreas, especially in relation to inflammation key players are little. This study investigated pancreatic autophagy status in PCOS rat model, with miR-223-3p and NF-κB levels as pivotal regulators of oxidative stress-autophagy axis, insulin, and β cell integrity. We then analyzed nanocurcumin effects as a putative anti-inflammatory nutraceutical on the disrupted parameters. Methods Nanocurcumin was characterized using transmission electron microscopy (TEM) and Fourier-transform IR (FT-IR) spectroscopy. Adult virgin Wistar rats were selected, and PCOS was induced using letrozole (1mg/kg). Nanocurcumin was ingested following letrozole. Sex hormones and insulin resistance were determined. miR-223-3p expression was determined using real-time PCR. Immunohistochemistry and Western blotting determined β cells, NF-κB, and autophagy markers p62 and LC3II. Results PCOS group showed significant disruptions in sex hormones and a double fold increase in glucose and insulin levels, exhibiting insulin resistance. Immunostaining confirmed around 46% deterioration of ß cell mass. Real-time PCR showed significant downregulation of miR-223-3p. Immunohistochemistry and Western blotting revealed a drastic upsurge of NF-κB, and autophagy markers p62 and LC3II, confirming bioinformatics target analysis. Interestingly, compared to PCOS group, nanocurcumin (200mg/kg) significantly upregulated miR-223-3p expression by 30%. It subsided NF-κB and autophagy eruption to restore ß cell mass and attenuate insulin resistance. Conclusion To the best of our knowledge, this study is the first to highlight the vital contribution of miR-223-3p and NF-κB levels in aggravating PCOS pancreatic autophagy and consequent impairments. It spots nanocurcumin potential as an inflammation and autophagy modulator, for possible better management of PCOS complications.

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supporting

confidence: 90%

Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Abuelezz¹,

Shabana²,

Rashed³

et al. 2021

JEP

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“…Curcumin-self-nanophospholipid dispersions were reported to enhance oral bioavailability of curcumin over conventional formulations in rats [189]. Curcumin nanoparticles prepared by a modified emulsion-diffusion-evaporation method was found to reduce fasting blood glucose and glycosylated hemoglobin levels significantly via increasing the expression of insulin and insulin receptor (IR) mRNAs in diabetic rats [190]. Curcumin-ZnO (10 mg/kg, for 21 days) nanoparticles were claimed to be more effective than curcumin nanoparticles (50 mg/kg, for 21 days) in diabetes therapy in terms of reduction of blood glucose, improvement in serum insulin, and activation of GLUT2 and glucokinase genes in pancreas and liver of type 2 diabetic rats [191].…”

Section: Curcuminmentioning

confidence: 99%

Plant-Based Antidiabetic Nanoformulations: The Emerging Paradigm for Effective Therapy

Dewanjee

Chakraborty

Mukherjee

et al. 2020

IJMS

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Diabetes mellitus is a life-threatening metabolic syndrome. Over the past few decades, the incidence of diabetes has climbed exponentially. Several therapeutic approaches have been undertaken, but the occurrence and risk still remain unabated. Several plant-derived small molecules have been proposed to be effective against diabetes and associated vascular complications via acting on several therapeutic targets. In addition, the biocompatibility of these phytochemicals increasingly enhances the interest of exploiting them as therapeutic negotiators. However, poor pharmacokinetic and biopharmaceutical attributes of these phytochemicals largely restrict their clinical usefulness as therapeutic agents. Several pharmaceutical attempts have been undertaken to enhance their compliance and therapeutic efficacy. In this regard, the application of nanotechnology has been proven to be the best approach to improve the compliance and clinical efficacy by overturning the pharmacokinetic and biopharmaceutical obstacles associated with the plant-derived antidiabetic agents. This review gives a comprehensive and up-to-date overview of the nanoformulations of phytochemicals in the management of diabetes and associated complications. The effects of nanosizing on pharmacokinetic, biopharmaceutical and therapeutic profiles of plant-derived small molecules, such as curcumin, resveratrol, naringenin, quercetin, apigenin, baicalin, luteolin, rosmarinic acid, berberine, gymnemic acid, emodin, scutellarin, catechins, thymoquinone, ferulic acid, stevioside, and others have been discussed comprehensively in this review.

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“…The biological effects and conditions assessed included anti-diabetic and antioxidant activity [63,97,98], diabetic neuropathic pain [99,100], diabetic cardiomyopathy [101,102], inflammation [103][104][105], and cataract in a diabetic rat model [106]. It was reported that nanoencapsulated curcumin produced anti-diabetic effects by increasing the number of insulin positive cells and the gene expression of insulin, alleviating STZ-induced β-cell damage and increasing the upregulation of the transcription factors pancreatic and duodenal homeobox 1 (Pdx-1), which play a critical role in pancreatic development, and NKx6.1 which is required for the development of β-cells [97,98]. One possible mechanism through which these effects could have been produced include curcumin inhibiting phosphodiesterases (PDEs) which degrade cyclic adenosine monophosphate (cAMP) [107].…”

Section: Diarylheptanoidmentioning

confidence: 99%

Nanopolyphenols: a review of their encapsulation and anti-diabetic effects

Rambaran

2020

SN Appl. Sci.

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Polyphenols are believed to possess numerous health benefits and can be grouped as phenolic acids, flavonoids or non-flavonoids. Research involving the synthesis of nanopolyphenols has attracted interest in the areas of functional food, nutraceutical and pharmaceutical development. This is in an effort to overcome current challenges which limit the application of polyphenols such as their rapid elimination, low water-solubility, instability at low pH, and their particle size. In the synthesis of nanopolyphenols, the type of nanocarrier used, the nanoencapsulation technique employed and the type of polymers that constitute the drug delivery system are crucial. For this review, all mentioned factors which can influence the therapeutic efficacy of nanopolyphenols were assessed. Their efficacy as anti-diabetic agents was also evaluated in 33 publications. Among these were phenolic acid (1), flavonoids (13), non-flavonoids (17) and polyphenolrich extracts (2). The most researched polyphenols were quercetin and curcumin. Nanoparticles were the main nanocarrier and the size of the nanopolyphenols ranged from 15 to 333 nm with encapsulation efficiency and drug loading capacities of 56-97.7% and 4.2-53.2%, respectively. The quantity of nanomaterial administered orally ranged from 1 to 300 mg/kg/day with study durations of 1-70 days. Most studies compared the effect of the nanopolyphenol to its freeform and, in all but three cases, significantly greater effects of the former were reported. Assessment of the polyphenol to understand its properties and the subsequent synthesis of its nanoencapsulated form using suitable nanocarriers, polymers and encapsulation techniques can result in effective therapeutic agents for the treatment of diabetes.

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Effects of nano-curcumin on gene expression of insulin and insulin receptor

Cited by 26 publications

References 34 publications

Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Nanocurcumin Modulates miR-223-3p and NF-κB Levels in the Pancreas of Rat Model of Polycystic Ovary Syndrome to Attenuate Autophagy Flare, Insulin Resistance and Improve ß Cell Mass

Plant-Based Antidiabetic Nanoformulations: The Emerging Paradigm for Effective Therapy

Nanopolyphenols: a review of their encapsulation and anti-diabetic effects

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