Effects of N-cadherin overexpression on the adhesion properties of embryonic stem cells

Karabekian, Zaruhi; Gillum, Nikki; Wong, Elissa W.P.; Sarvazyan, Narine

doi:10.4161/cam.3.3.8399

Cited by 13 publications

(7 citation statements)

References 31 publications

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“…Therefore, the exact role of Cx43 in metastasis is still to be investigated more in detail than ever. In terms of relationships between connexins and cell adhesion molecules, accumulating evidence indicated that cell adhesion molecules have been implicated in connexins docking, as L-CAM [24], E-cadherin [25], and N-cadherin [26] activities appear to be important for GJIC. In addition, the cadherin repertoire can determine the type of GJIC.…”

Section: Cx43 In Mlnmentioning

confidence: 98%

Expression and significance of Cx43 and E-cadherin in gastric cancer and metastatic lymph nodes

Tang

Peng

et al. 2010

Med Oncol

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Connexin 43(Cx43) and E-cadherin are concurrently expressed in many tumors and were ever classified as tumor suppressors in primary tumors (PT), whereas recent studies showed that these two proteins played specific roles in tumor metastasis. The aim of our study is to determine the expression of Cx43 and E-cadherin in primary gastric tumors (PTs) and matched metastatic lymph nodes (MLNs) and to explore the clinical and pathological implications of expression of these proteins. Immunohistochemical assay was conducted to detect the expression of Cx43 and E-cadherin in PTs and MLNs, and the clinical and pathological implications were analyzed by statistical methods. In PTs, the expression of Cx43 and E-cadherin was significantly reduced, compared to adjacent normal tissues (P < 0.01). The expression of Cx43 and E-cadherin was significantly increased in MLNs compared with PTs (P < 0.01 and P < 0.01, for Cx43 and E-cadherin, respectively), and some Cx43 and E-cadherin-negative PTs developed Cx43 and E-cadherin-positive MLNs. Furthermore, reduced expression of both Cx43 and E-cadherin significantly correlated with poor differentiation, advanced TNM stage, and lymph note metastasis of gastric cancers. Cx43 and E-cadherin expression significantly correlated with each other. We concluded that concurrent reduction in Cx43 and E-cadherin may contribute to the occurrence of gastric cancer. However, concurrent increased expression of Cx43 and E-cadherin may contribute to the efficient metastasis of gastric cancer to the lymph nodes.

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Section: Cx43 In Mlnmentioning

confidence: 98%

Expression and significance of Cx43 and E-cadherin in gastric cancer and metastatic lymph nodes

Tang

Peng

et al. 2010

Med Oncol

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“…hiPSC- and hESC-CMs were, however, connected by 'primitive ICDs’ [ 58 ], which may contribute to the immature electrical properties of these cells. Unlike Cx43, over-expression of N-cadherin [ 80 ] in mESCs does not interfere with the formation of functional CMs. In fact, mESC-CMs that over-express N-cadherin show increased levels of Cx43 [ 80 ], supporting the idea that adherens junction formation drives connexin expression [ 81 ].…”

Section: State Of Hpsc-cms During Differentiationmentioning

confidence: 99%

Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes

et al. 2014

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Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are the most promising source of cardiomyocytes (CMs) for experimental and clinical applications, but their use is largely limited by a structurally and functionally immature phenotype that most closely resembles embryonic or fetal heart cells. The application of physical stimuli to influence hPSC-CMs through mechanical and bioelectrical transduction offers a powerful strategy for promoting more developmentally mature CMs. Here we summarize the major events associated with in vivo heart maturation and structural development. We then review the developmental state of in vitro derived hPSC-CMs, while focusing on physical (electrical and mechanical) stimuli and contributory (metabolic and hypertrophic) factors that are actively involved in structural and functional adaptations of hPSC-CMs. Finally, we highlight areas for possible future investigation that should provide a better understanding of how physical stimuli may promote in vitro development and lead to mechanistic insights. Advances in the use of physical stimuli to promote developmental maturation will be required to overcome current limitations and significantly advance research of hPSC-CMs for cardiac disease modeling, in vitro drug screening, cardiotoxicity analysis and therapeutic applications.

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“…28 Amplification using the mtDNA primers was done with 0.25 ng of total DNA. A standard curve (5, 1, 0.5, 0.05, and 0.005 ng of total DNA/reaction) was performed with the 18S primers.…”

Section: Dna Isolationmentioning

confidence: 99%

Restoration of Mitochondrial Integrity, Telomere Length, and Sensitivity to Oxidation by In Vitro Culture of Fuchs' Endothelial Corneal Dystrophy Cells

Gendron

Thériault

Proulx

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Citation: Gendron SP, Thériault M, Proulx S, Brunette I, Rochette PJ. Restoration of mitochondrial integrity, telomere length, and sensitivity to oxidation by in vitro culture of Fuchs' endothelial corneal dystrophy cells. Invest Ophthalmol Vis Sci. 2016;57:5926-5934. DOI:10.1167/ iovs.16-20551 PURPOSE. Fuchs' endothelial corneal dystrophy (FECD), a degenerative disease of the corneal endothelium that leads to vision loss, is a leading cause of corneal transplantation. The cause of this disease is still unknown, but the implication of oxidative stress is strongly suggested. In this study, we analyzed the impact of FECD on mitochondrial DNA (mtDNA) integrity and telomere length, both of which are affected by the oxidative status of the cell. METHODS.We compared the levels of total mtDNA, mtDNA common deletion (4977 bp), and relative telomere length in the corneal endothelial cells of fresh Descemet's membraneendothelium explants and cultured cells from healthy and late stage FECD subjects. Oxidantantioxidant gene expression and sensitivity to ultraviolet A (UVA)-and H 2 O 2 -induced cell death were assessed in cultured cells. RESULTS.Our results revealed increased mtDNA levels and telomere shortening in FECD explants. We also found that cell culture restores a normal phenotype in terms of mtDNA levels, telomere length, oxidant-antioxidant gene expression balance, and sensitivity to oxidative stress-induced cell death in the FECD cells compared with the healthy cells.CONCLUSIONS. Taken together, these results bring new evidence of the implication of oxidative stress in FECD. They also show that FECD does not evenly affect the integrity of corneal endothelial cells and that cell culture can rehabilitate the molecular phenotypes related to oxidative stress by selecting the more functional FECD cells.

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Effects of N-cadherin overexpression on the adhesion properties of embryonic stem cells

Cited by 13 publications

References 31 publications

Expression and significance of Cx43 and E-cadherin in gastric cancer and metastatic lymph nodes

Expression and significance of Cx43 and E-cadherin in gastric cancer and metastatic lymph nodes

Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes

Restoration of Mitochondrial Integrity, Telomere Length, and Sensitivity to Oxidation by In Vitro Culture of Fuchs' Endothelial Corneal Dystrophy Cells

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