Effects of N-acetylcysteine on isolated skeletal muscle contractile properties after an acute bout of aerobic exercise

Jannig, Paulo R.; Alves, Christiano Robles Rodrigues; Voltarelli, Vanessa Azevedo; Bozi, Luíz Henrique Marchesi; Vieira, J.S.; Brum, Patrícia Chakur; Bechara, Luiz R. G.

doi:10.1016/j.lfs.2017.10.012

Cited by 5 publications

(10 citation statements)

References 33 publications

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“…While conducting these experiments, we observed that tumor‐bearing rats displayed atrophy in glycolytic muscles, but not in soleus , a muscle with a high prevalence of type I (oxidative) fibers. Consistent with these findings, other studies demonstrated that type I fibers have a higher oxidative capacity and lower response to cachexia and fatigue than type II fibers 17‐20 . These findings suggest that oxidative muscle has intrinsic mechanisms to protect from the cachectic factors.…”

Section: Introductionsupporting

confidence: 74%

“…We find here that intrinsic muscle oxidative capacity determines the response to the cachectic effects, with type I oxidative fibers not showing significant atrophy in this severe cancer model. This intrinsic protection of oxidative muscle fibers could be attributed, at least in part, to the higher innate antioxidant capacity 18,19 . Interestingly, aerobic exercise training, a strategy able to increase intramuscular antioxidant capacity, also attenuates atrophy and improves the function of glycolytic muscles in different models of cancer cachexia 8 .…”

Section: Resultsmentioning

confidence: 99%

“…The muscles were mounted between force transducers in a tissue bath containing aerated Krebs Ringer's buffer (137 mM NaCl, 5 mM KCl, 2 mM CaCl 2 , 1 mM KH 2 PO 4 , 1 mM MgSO 4 , 24 mM NaHCO 3 , 11 mM C 6 H 12 O 6 ; 22℃; pH 7.4; 95% O 2 and 5% CO 2 ) and platinum‐based electrodes for electrical stimulation. Force output was recorded after successive electrical stimulation as previously described 18 . After identifying the optimal muscle length, a force‐frequency protocol was then conducted.…”

Section: Methodsmentioning

confidence: 99%

“…Force output was recorded for both force‐frequency and fatigue inducing protocols. Data were presented as absolute force production and also normalized by total muscle cross‐sectional area, which was mathematically estimated by dividing the muscle mass by the product of optimum fiber length and the density of muscle as previously described 18 …”

Section: Methodsmentioning

confidence: 99%

“…Force output was recorded after successive electrical stimulation as previously described. 18 After identifying the optimal muscle length, a force-frequency protocol was then conducted. Stimuli were applied with successive frequency increases from 10 to 100 Hz.…”

Section: Isolated Skeletal Muscle Contractilitymentioning

confidence: 99%

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Cancer‐induced muscle atrophy is determined by intrinsic muscle oxidative capacity

et al. 2021

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We tested the hypothesis that cancer cachexia progression would induce oxidative post‐translational modifications (Ox‐PTMs) associated with skeletal muscle wasting, with different responses in muscles with the prevalence of glycolytic and oxidative fibers. We used cysteine‐specific isotopic coded affinity tags (OxICAT) and gel‐free mass spectrometry analysis to investigate the cysteine Ox‐PTMs profile in the proteome of both plantaris (glycolytic) and soleus (oxidative) muscles in tumor‐bearing and control rats. Histological analysis revealed muscle atrophy in type II fibers in plantaris muscle, with no changes in plantaris type I fibers and no differences in both soleus type I and II fibers in tumor‐bearing rats when compared to healthy controls. Tumor progression altered the Ox‐PTMs profile in both plantaris and soleus. However, pathway analysis including the differentially oxidized proteins revealed tricarboxylic acid cycle and oxidative phosphorylation as main affected pathways in plantaris muscle from tumor‐bearing rats, while the same analysis did not show main metabolic pathways affected in the soleus muscle. In addition, cancer progression affected several metabolic parameters such as ATP levels and markers of oxidative stress associated with muscle atrophy in plantaris muscle, but not in soleus. However, isolated soleus from tumor‐bearing rats had a reduced force production capacity when compared to controls. These novel findings demonstrate that tumor‐bearing rats have severe muscle atrophy exclusively in glycolytic fibers. Cancer progression is associated with cysteine Ox‐PTMs in the skeletal muscle, but these modifications affect different pathways in a glycolytic muscle compared to an oxidative muscle, indicating that intrinsic muscle oxidative capacity determines the response to cancer cachectic effects.

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Section: Introductionsupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Isolated Skeletal Muscle Contractilitymentioning

confidence: 99%

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