Effects of Myo-Inositol and Thiamine on Micropropagation of GF677 (Peach × Almond Hybrid)

Sepahvand, Sadegh; Ebadi, Abbas; Kamali, Koorosh; Ghaemmaghami, S. A.

doi:10.5539/jas.v4n2p275

Cited by 7 publications

(6 citation statements)

References 11 publications

(6 reference statements)

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“…Our results were in accordance with the findings of Hepaksoy and Tanrisever (2004) on sweet cherry, Ruzic and Vujovic (2007) and Dolgov et al (2010) on Prunus domestica that all indicated that the highest shooting rate was obtained in the combination of BAP and IBA treatment. The present study findings are in opposite of Sepahvand et al (2011) on GF677 vegetative rootstock since they introduced BAP and NAA combination as the most appropriate compound for shooting. Here, the combination of BAP and IBA was the most effective treatment which is in accordance with other researchers’ findings conveyed that the effective shooting depends on the cytokinin type and concentration as well as suitable concentrations of cytokinin–auxin, i.e., using BAP along with low amounts of IBA is favorable for shooting ( Sedlák and Paprstein, 2007 ; Ruzic and Vujovic, 2008 ).…”

Section: Discussioncontrasting

confidence: 82%

Mathematical Modeling and Optimizing of in Vitro Hormonal Combination for G × N15 Vegetative Rootstock Proliferation Using Artificial Neural Network-Genetic Algorithm (ANN-GA)

et al. 2017

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The efficiency of a hybrid systems method which combined artificial neural networks (ANNs) as a modeling tool and genetic algorithms (GAs) as an optimizing method for input variables used in ANN modeling was assessed. Hence, as a new technique, it was applied for the prediction and optimization of the plant hormones concentrations and combinations for in vitro proliferation of Garnem (G × N15) rootstock as a case study. Optimizing hormones combination was surveyed by modeling the effects of various concentrations of cytokinin–auxin, i.e., BAP, KIN, TDZ, IBA, and NAA combinations (inputs) on four growth parameters (outputs), i.e., micro-shoots number per explant, length of micro-shoots, developed callus weight (CW) and the quality index (QI) of plantlets. Calculation of statistical values such as R2 (coefficient of determination) related to the accuracy of ANN-GA models showed a considerably higher prediction accuracy for ANN models, i.e., micro-shoots number: R2 = 0.81, length of micro-shoots: R2 = 0.87, CW: R2 = 0.88, QI: R2 = 0.87. According to the results, among the input variables, BAP (19.3), KIN (9.64), and IBA (2.63) showed the highest values of variable sensitivity ratio for proliferation rate. The GA showed that media containing 1.02 mg/l BAP in combination with 0.098 mg/l IBA could lead to the optimal proliferation rate (10.53) for G × N15 rootstock. Another objective of the present study was to compare the performance of predicted and optimized cytokinin–auxin combination with the best optimized obtained concentrations of our other experiments. Considering three growth parameters (length of micro-shoots, micro-shoots number, and proliferation rate), the last treatment was found to be superior to the rest of treatments for G × N15 rootstock in vitro multiplication. Very little difference between the ANN predicted and experimental data confirmed high capability of ANN-GA method in predicting new optimized protocols for plant in vitro propagation.

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Section: Discussioncontrasting

confidence: 82%

Mathematical Modeling and Optimizing of in Vitro Hormonal Combination for G × N15 Vegetative Rootstock Proliferation Using Artificial Neural Network-Genetic Algorithm (ANN-GA)

et al. 2017

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“…Our results showed that the increase in myo-inositol concentration inhibits the shoot length of both rootstocks which is in agreement with the results by Sepahvand et al (2012) using the peach rootstock GF-677. However, Al-Sulaiman (2010) found no significant effects of myoinositol on the shoot length of Ziziphus spina-christi.…”

Section: Rootstocksupporting

confidence: 92%

Indole-3-butyric acid and myo-inositol impacts on in vitro rooting of the cherry rootstocks CAB-6P and Gisela 6

Sarropoulou¹,

Dimassi-Theriou²,

Therios³

2013

Biol. plant.

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The present study investigates the effects of indole-3-butyric acid (IBA) alone and in combination with myo-inositol on in vitro rooting and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens). For the CAB-6P rootstock, the best results for root number (6.31), fresh mass (FM), dry mass (DM), and rooting percentage (100 %) were obtained on Murashige and Skoog (MS) medium with 2 mg dm -3 IBA and maximum root length (30.57 mm) was obtained at 1 mg dm -3 IBA. Myo-inositol suppressed the positive effects of IBA on root length. In the Gisela 6 explants, the inclusion of 2 mg dm -3 IBA together with 0.5 mg dm -3 of myo-inositol in the culture medium significantly enhanced root number (9.91) and root FM and DM. The root length was maximum in the combination of the lowest IBA and myo-inositol concentrations (0.5 mg dm -3 ). The rooting percentage was the greatest (100 %) with the application of 1 mg dm -3 IBA alone. In both explants, the application of IBA alone or in combination with myo-inositol resulted in a lower leaf proline content in comparison with the control (without growth regulators). The maximum leaf chlorophyll content was at 1 mg dm -3 IBA in the CAB-6P whereas at 2 mg dm -3 IBA and 1 mg dm -3 myo-inositol in Gisela 6. Addition of myo-inositol mostly increased sugar content in comparison with control or IBA alone in both rootstocks.

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“…Based on our results, it is possible that IBA positively interacts with the two vitamins promoting AR-elongation as the necessary premise for ARbranching. In accordance, a positive thiamine and IBA interaction in favouring AR-elongation has been demonstrated in the micropropagation of GF677 (peach × almond hybrid) (Sepahvand et al 2012). Moreover, AR-elongation was also favoured by specific combinations of IBA and myo-inositol in cherry rootstocks CAB-6P and Gisela 6 (Sarropoulou et al 2013).…”

Section: Myo-inositol and Thiamine Cooperate With Exogenous Iba In Ensupporting

confidence: 68%

“…Synergistic interactions between thiamine and auxins have been reported for different plant rooting systems, as Jatropha curcas and Tectona grandis cuttings (Dhillon et al 2011, and references therein), and GF677 (peach x almond hybrid) shoot microcuttings. In the latter, significant rooting increments were detected at different IBA levels, but always when thiamine was present even at low levels (Sepahvand et al 2012). Thiamine may also interact positively with cytokinins.…”

Section: Introductionmentioning

confidence: 93%

EIN2 and COI1 control the antagonism between ethylene and jasmonate in adventitious rooting of Arabidopsis thaliana thin cell layers

Betti

Rovere

Ronzan

et al. 2019

Plant Cell Tiss Organ Cult

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Auxins induce adventitious roots (ARs) in numerous culture-systems, and indole-3-butyric acid (IBA) is frequently the best AR-inducer. Vitamin requirements vary according to species, explant, and culture-conditions. Arabidopsis thaliana thin cell layers (AtTCLs) are uncapable of AR-formation on hormone-free medium containing thiamine and myo-inositol, whereas ARs are induced when IBA (10 μM), with/without kinetin (Kin, 0.1 μM), is added. The research first aim was to determine whether a synergism between IBA and myo-inositol and thiamine was necessary for AR-formation. Results showed that IBA induced AR-formation without myo-inositol and thiamine, but better when both vitamins were also present. Deciphering hormonal action on AR formation under optimal vitamin content would be essential for improving the AR process. Ethylene (ET)/jasmonic acid (JA) signaling cross-talk has been demonstrated as being involved in AR-formation in IBA + Kincultured AtTCLs, by using ein3eil1 and coi1-16 mutants. ETHYLENE INSENSITIVE3 (EIN3)/EIN3-LIKE1 (EIL1) are positive regulators of ethylene (ET)-signaling, whereas CORONATINE INSENSITIVE1 (COI1) is involved in JA-signaling. The ETHYLENE INSENSITIVE2 (EIN2) protein activates EIN3/EIL1 in ET-presence. To understand whether EIN2 was also involved, the AR-response of ein2-1 and coi1-16 TCLs was evaluated adding the ET-precursor 1-aminocyclopropane-1-carboxylic acid (ACC, 0.1 μM) and/or the JA-donor methyl jasmonate (JAMe, 0.01 μM) to IBA + vitamins-containing medium. AR-formation was enhanced by JAMe, reduced by ACC, but unchanged by JAMe + ACC in the wild type TCLs, whereas remained similarly low in ein2-1 and coi1-16 under all treatments. Collectively, these results demonstrate that the antagonism between JA and ET in AR-formation from AtTCLs involves a cross-talk by EIN2 and COI1. Key messageThe reciprocal regulation of ethylene and jasmonate perception through EIN2 and COI1 cross-talk is involved in root formation and branching in Arabidopsis stem explants, cultured with IBA, myo-inositol and thiamine.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Effects of Myo-Inositol and Thiamine on Micropropagation of GF677 (Peach × Almond Hybrid)

Cited by 7 publications

References 11 publications

Mathematical Modeling and Optimizing of in Vitro Hormonal Combination for G × N15 Vegetative Rootstock Proliferation Using Artificial Neural Network-Genetic Algorithm (ANN-GA)

Mathematical Modeling and Optimizing of in Vitro Hormonal Combination for G × N15 Vegetative Rootstock Proliferation Using Artificial Neural Network-Genetic Algorithm (ANN-GA)

Indole-3-butyric acid and myo-inositol impacts on in vitro rooting of the cherry rootstocks CAB-6P and Gisela 6

EIN2 and COI1 control the antagonism between ethylene and jasmonate in adventitious rooting of Arabidopsis thaliana thin cell layers

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