Mathematical Modeling and Optimizing of in Vitro Hormonal Combination for G × N15 Vegetative Rootstock Proliferation Using Artificial Neural Network-Genetic Algorithm (ANN-GA)
Abstract:The efficiency of a hybrid systems method which combined artificial neural networks (ANNs) as a modeling tool and genetic algorithms (GAs) as an optimizing method for input variables used in ANN modeling was assessed. Hence, as a new technique, it was applied for the prediction and optimization of the plant hormones concentrations and combinations for in vitro proliferation of Garnem (G × N15) rootstock as a case study. Optimizing hormones combination was surveyed by modeling the effects of various concentrati… Show more
“…The pear rootstock responses to in vitro nutrients has been reported that varies with the macro-nutrients levels in the culture medium [10]. Comparison of our comprehensive results to the previous results [10, 15, 50, 51] showed for the first time that the concentrations of macro- and micro-nutrients depend highly on the concentrations of used hormones as their interaction could determine the quality of plantlets. Arab et al [15] predicted and maximized the number and length of in vitro regenerated shoots by decreasing NH 4 + concentration and optimizing NO 3 − concentration, simultaneously.…”
Section: Discussionsupporting
confidence: 66%
“…AI has recently been successfully and progressively applied in plant bio-researches [48] as well as for predicting the optimal plant tissue culture conditions [49] and media components [10, 15, 50, 51]. The in vitro plant tissues development is under control of the culture media nutrients.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, different media are needed for optimal growth of Pyrus genotypes because of the incidence of the physiological disorders like hyperhydricity and necrosis at the explant multiplication stage. In order to design an optimized culture medium, use of a trustworthy mathematical modeling and optimization technique is necessary to achieve an optimal and efficient growth [10, 15, 50, 51]. Diverse statistical techniques have been used previously to design new and effective plant tissue culture media [10, 15, 32, 50, 51, 57].…”
Section: Discussionmentioning
confidence: 99%
“…In order to design an optimized culture medium, use of a trustworthy mathematical modeling and optimization technique is necessary to achieve an optimal and efficient growth [10, 15, 50, 51]. Diverse statistical techniques have been used previously to design new and effective plant tissue culture media [10, 15, 32, 50, 51, 57]. RSM and MLR have been constantly applied to optimize new in vitro media for pear genotypes [10, 19].…”
BackgroundPredicting impact of plant tissue culture media components on explant proliferation is important especially in commercial scale for optimizing efficient culture media. Previous studies have focused on predicting the impact of media components on explant growth via conventional multi-layer perceptron neural networks (MLPNN) and Multiple Linear Regression (MLR) methods. So, there is an opportunity to find more efficient algorithms such as Radial Basis Function Neural Network (RBFNN) and Gene Expression Programming (GEP). Here, a novel algorithm, i.e. GEP which has not been previously applied in plant tissue culture researches was compared to RBFNN and MLR for the first time. Pear rootstocks (Pyrodwarf and OHF) were used as case studies on predicting the effect of minerals and some hormones in the culture medium on proliferation indices.ResultsGenerally, RBFNN and GEP showed extremely higher performance accuracy than the MLR. Moreover, GEP models as the most accurate models were optimized using genetic algorithm (GA). The improvement was mainly due to the RBFNN and GEP strong estimation capability and their superior tolerance to experimental noises or improbability.ConclusionsGEP as the most robust and accurate prospecting procedure to achieve the highest proliferation quality and quantity has also the benefit of being easy to use.
“…The pear rootstock responses to in vitro nutrients has been reported that varies with the macro-nutrients levels in the culture medium [10]. Comparison of our comprehensive results to the previous results [10, 15, 50, 51] showed for the first time that the concentrations of macro- and micro-nutrients depend highly on the concentrations of used hormones as their interaction could determine the quality of plantlets. Arab et al [15] predicted and maximized the number and length of in vitro regenerated shoots by decreasing NH 4 + concentration and optimizing NO 3 − concentration, simultaneously.…”
Section: Discussionsupporting
confidence: 66%
“…AI has recently been successfully and progressively applied in plant bio-researches [48] as well as for predicting the optimal plant tissue culture conditions [49] and media components [10, 15, 50, 51]. The in vitro plant tissues development is under control of the culture media nutrients.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, different media are needed for optimal growth of Pyrus genotypes because of the incidence of the physiological disorders like hyperhydricity and necrosis at the explant multiplication stage. In order to design an optimized culture medium, use of a trustworthy mathematical modeling and optimization technique is necessary to achieve an optimal and efficient growth [10, 15, 50, 51]. Diverse statistical techniques have been used previously to design new and effective plant tissue culture media [10, 15, 32, 50, 51, 57].…”
Section: Discussionmentioning
confidence: 99%
“…In order to design an optimized culture medium, use of a trustworthy mathematical modeling and optimization technique is necessary to achieve an optimal and efficient growth [10, 15, 50, 51]. Diverse statistical techniques have been used previously to design new and effective plant tissue culture media [10, 15, 32, 50, 51, 57]. RSM and MLR have been constantly applied to optimize new in vitro media for pear genotypes [10, 19].…”
BackgroundPredicting impact of plant tissue culture media components on explant proliferation is important especially in commercial scale for optimizing efficient culture media. Previous studies have focused on predicting the impact of media components on explant growth via conventional multi-layer perceptron neural networks (MLPNN) and Multiple Linear Regression (MLR) methods. So, there is an opportunity to find more efficient algorithms such as Radial Basis Function Neural Network (RBFNN) and Gene Expression Programming (GEP). Here, a novel algorithm, i.e. GEP which has not been previously applied in plant tissue culture researches was compared to RBFNN and MLR for the first time. Pear rootstocks (Pyrodwarf and OHF) were used as case studies on predicting the effect of minerals and some hormones in the culture medium on proliferation indices.ResultsGenerally, RBFNN and GEP showed extremely higher performance accuracy than the MLR. Moreover, GEP models as the most accurate models were optimized using genetic algorithm (GA). The improvement was mainly due to the RBFNN and GEP strong estimation capability and their superior tolerance to experimental noises or improbability.ConclusionsGEP as the most robust and accurate prospecting procedure to achieve the highest proliferation quality and quantity has also the benefit of being easy to use.
“…In Rumelhart et al ( 1986 ), error backpropagation feature was introduced, a process that finds the gradients of the neurons' weights to adjust them, from the last layer to the first one. ANNs can also be found in multiple applications for plant science, e.g., leaf area index calculation (Yuan et al, 2017 ), rootstock genetics (Arab et al, 2017 ) or disease detection (Pérez-Bueno et al, 2016 ). For this reason, a deep analysis of how these algorithms and their multiple parameter settings behave with hyperspectral data is desirable, as they arise as powerful tools for the varietal classification objective.…”
Grapevine varietal classification is an important plant phenotyping issue for grape growing and wine industry. This task has been achieved from destructive techniques like classic ampelography and DNA analysis under laboratory conditions. This work displays a new approach for the classification of a high number of grapevine (Vitis vinifera L.) varieties under field conditions using on-the-go hyperspectral imaging and different machine learning algorithms. On-the-go imaging was performed under natural illumination using a hyperspectral camera mounted on an all-terrain vehicle at 5 km/h. Spectra were acquired over two different leaf phenological stages on the canopy of 30 different varieties on a commercial vineyard located in La Rioja, Spain. A total of 1,200 spectral samples were generated. Support vector machines (SVM) and artificial neural networks (multilayer perceptrons, MLP) were used for the development of a large number of models, testing different algorithm parameters and spectral pre-processing techniques. Both classifiers yielded notable performance values and were able to train models with recall F1 scores and area under the receiver operating characteristic curve marks up to 0.99 for 5-fold cross validation. Statistical analyses supported that the best SVM kernel was linear and the best activation function for MLP was the hyperbolic tangent function. The prediction performance for individual varieties of MLP ranged from 0.94 to 0.99, displaying low levels of variability. In the case of SVM, slightly higher differences were obtained, ranging from 0.83 to 0.97 for individual varieties. These results support the possibility of deploying an on-the-go hyperspectral imaging system in the field capable of successfully classifying leaves from different grapevine varieties. This technology could thus be considered as a new useful non-destructive tool for plant phenotyping under field conditions.
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