Effects of Moringa oleifera Lam Extract (MOLE) in the heat shock protein 70 expression and germ cell apoptosis on experimentally induced cryptorchid testes of rats

Tekayev, Muhammetnur; Bostancıeri, Nuray; Saadat, Khandakar A.S.M.; Türker, Mehmet; Yüncü, Mehmet; Ulusal, Hasan; Çiçek, Hülya; Arman, Kaifee

doi:10.1016/j.gene.2018.11.091

Cited by 13 publications

(8 citation statements)

References 28 publications

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“…In the present study, we examined the localization and expression of the AQP7, 8, and 9 proteins and their mRNAs in the efferent ductules and epididymis of normal and cryptorchid dogs. To clarify the possible roles played by the examined AQPs in the epididymal tissue, we analyzed the expression of Hsp70 and caspase-3, which are well known to be involved, among numerous other factors, in the heat stress and oxidative mechanisms related to cryptorchidism [53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Aquaporins Are Differentially Regulated in Canine Cryptorchid Efferent Ductules and Epididymis

Squillacioti

Mirabella

Liguori

et al. 2021

Animals

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The efferent ductules and the epididymis are parts of the male reproductive system where spermatozoa mature. Specialized epithelial cells in these ducts contribute to the transport of fluids produced by spermatozoa’s metabolic activity. Aquaporins (AQPs) have been demonstrated to be expressed in the spermatozoan membrane and testis epithelial cells, where they contribute to regulating spermatozoan volume and transit through environments of differing osmolality. Due to the lack of detailed literature regarding AQP expression in the canine male genital tract, the aim of this study was to investigate both the distribution and expression of AQP7, AQP8, and AQP9 in the efferent ductules and epididymal regions (caput, corpus, and cauda) from normal and cryptorchid dogs by using immunohistochemistry, Western blotting, and real-time reverse transcription polymerase chain reaction (RT-PCR). Our results show different patterns for the distribution and expression of the examined AQPs, with particular evidence of their upregulation in the caput and downregulation in the cauda region of the canine cryptorchid epididymis. These findings are associated with a modulation of Hsp70 and caspase-3 expression, suggesting the participation of AQPs in the luminal microenvironment modifications that are peculiar characteristics of this pathophysiological condition.

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Section: Discussionmentioning

confidence: 99%

Aquaporins Are Differentially Regulated in Canine Cryptorchid Efferent Ductules and Epididymis

Squillacioti

Mirabella

Liguori

et al. 2021

Animals

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“…Moreover, several studies were progressed with high doses, especially maca capsule and extract were treated using more than 500 mg/kg [ 64 , 65 ]. Two of three studies that reported the efficacy of Moringa oleifera used more than 100 mg/kg [ 66 , 67 ]. Additionally, Nigella sativa seed powder was treated using 300 mg/kg [ 69 ], while Diallyl sulfide and Matricaria chamomilla hydroethanolic extract were treated using over 200 mg/kg [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…(400, 800 mg/kg for 2 weeks) treated to cryptorchidism induced SD rats. The results revealed increases in germinal cell layer thickness, diameter of seminiferous tubules, testis weight index, and testicular weight, but decreases in perivascular fibrosis and apoptotic cell detection [ 67 ]. The morphological changes were observed to be related to increased expressions of SOD and lowered levels of HSP70 and MDA.…”

Section: Plant Extract and Infertilitymentioning

confidence: 99%

Role of Antioxidant Natural Products in Management of Infertility: A Review of Their Medicinal Potential

Noh

Kim

et al. 2020

Antioxidants

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Infertility, a couple’s inability to conceive after one year of unprotected regular intercourse, is an important issue in the world. The use of natural products in the treatment of infertility has been considered as a possible alternative to conventional therapies. The present study aimed to investigate the effects and the mechanisms of various natural products on infertility. We collected articles regarding infertility and natural products using the research databases PubMed and Google Scholar. Several natural products possess antioxidant properties and androgenic activities on productive factors and hormones. Antioxidants are the first defense barrier against free radicals produced by oxidative stress (OS). They remove reactive oxygen stress (ROS), reducing insulin resistance, total cholesterol, fat accumulation, and cancer growth. Moreover, various natural products increase endometrial receptivity and fertility ability showing androgenic activities on productive factors and hormones. For example, Angelica keiskei powder and Astragalus mongholicus extract showed anti-infertility efficacies in males and females, respectively. On the other hand, adverse effects and acute toxicity of natural products were also reported. Tripterygium glycoside decreased fertility ability both in males and females. Results indicate that management of infertility with natural products could be beneficial with further clinical trials to evaluate the safety and effect.

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“…Evidence suggests that other conditions or pathologies related to oxidative stress may benefit from antioxidant therapy. Rats with surgically induced unilateral cryptorchidism showed high levels of MDA, apoptosis, and heat-shock proteins (HSP70), as well as decreased levels of SOD (Tekayev et al, 2019). Using the extract of Moringa oleifera, a powerful natural antioxidant rich in β-carotene, riboflavin, folic and nicotinic acids and vitamins A, B C and E, the authors found an important reduction in oxidative stress, in the expression of HSP70 (a heat shock protein which is involved in sperm cell compromise) and apoptosis of germ cells (Tekayev et al, 2019).…”

Section: Oxidative Stress and Experimental Modelsmentioning

confidence: 99%

“…Rats with surgically induced unilateral cryptorchidism showed high levels of MDA, apoptosis, and heat-shock proteins (HSP70), as well as decreased levels of SOD (Tekayev et al, 2019). Using the extract of Moringa oleifera, a powerful natural antioxidant rich in β-carotene, riboflavin, folic and nicotinic acids and vitamins A, B C and E, the authors found an important reduction in oxidative stress, in the expression of HSP70 (a heat shock protein which is involved in sperm cell compromise) and apoptosis of germ cells (Tekayev et al, 2019). A study sought to evaluate the role of 3 compounds (dihydroquercetin, p-tyrosol, dibornol) in rat infertility induced by pathospermia (Borovskaya et al, 2018).…”

Section: Oxidative Stress and Experimental Modelsmentioning

confidence: 99%

Review on the role of antioxidant supplementation against oxidative stress: a human and animal approach to male fertility

Adami

Maciel

Losano

2022

RSD

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Male infertility is one important factor among the multifactorial causes of couple infertility, being oxidative stress one of the main related sources. Sperm is a specialized cell extremely susceptible to stress. To understand and mitigate this event, many studies have used different antioxidants, orally or in vitro supplementation, trying to improve sperm quality and function. Considering the extensive available literature regarding approaches and attempts to solve male fertility issues, the aim of this review is evaluating the effects of antioxidant supplementation on sperm, in both humans and experimental models with animals. This review selected original data from PubMed. The keywords used were: antioxidant, sperm, male fertility, antioxidant supplementation, male infertility; and the term "rodents" was added to the descriptors “antioxidant” and “male fertility”. Only studies published in indexed journals, in English, between 2015 and 2019 were included. This review involves i) human sperm and ii) rodent sperm. For the human approach, the search retrieved 496 articles and 80 were included, among which 28 studies were of in vitro antioxidant supplementation, 19 involved oral antioxidant supplementation and the remaining 33 concerned quantification of oxidants and antioxidants already present in the seminal samples. For the rodent approach, 152 articles were retrieved and 52 were included: 3 of varicocele, 11 of diabetes, 10 of therapeutic drugs, 3 of physical exercise, 10 of environmental exposure and 3 of heat stress. The remaining studies involved oxidative stress status in experimental models. Antioxidants use for reproductive purposes is increasing in an attempt to achieve better gametes and embryos. Vitamins C, B and E, selenium and zinc are the most commonly used antioxidants, with remarkable evidences in improving pathophysiological seminal conditions.

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Effects of Moringa oleifera Lam Extract (MOLE) in the heat shock protein 70 expression and germ cell apoptosis on experimentally induced cryptorchid testes of rats

Cited by 13 publications

References 28 publications

Aquaporins Are Differentially Regulated in Canine Cryptorchid Efferent Ductules and Epididymis

Aquaporins Are Differentially Regulated in Canine Cryptorchid Efferent Ductules and Epididymis

Role of Antioxidant Natural Products in Management of Infertility: A Review of Their Medicinal Potential

Review on the role of antioxidant supplementation against oxidative stress: a human and animal approach to male fertility

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