Effects of Moderate Exercise Training on Splenocyte Inflammatory Cytokine Production in High Fat Diet Induced Obese Mice

Abstract

The present research was conducted to investigate the effect of moderate exercise training on splenocyte inflammatory cytokines production in high fat diet-induced obese mice. To determine the effects of exercise training and low fat diet on splenocyte, C57BL/6 male mice (4 weeks aged, n=20) were fed a high fat diet (45% fat diet) for 5 weeks so that obesity was achieved intentionally. These obese mice were then divided into 2 groups; HLC (low fat diet and control n=10), and HLE (low fat diet and exercise n=10). HLE mice performed 8 weeks of exercise training on a motorized treadmill by running for 30-60 min/day at 10-22 m/min, 0% grade, five times per week. After exercise training, all the splenocyte was collected and Con A (concanvalin A, 10μg/ml) was used to stimulate the cells, after which IL-1β, IL-6, TNF-α were measured by bio plex. Independent t-test was used and p value under 0.05 was considered a statistical significance. Splenocyte IL-1β, IL-6, TNF-α production of HLE stimulated by Con A was significantly lower than that of HLC (p<0.01). These findings suggest that moderate exercise has beneficial effects on splenocyte inflammatory cytokines in high fat diet induced obese mice.

Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice

“…Such result was the same with the weight reduction and according to previous studies [ 9 , 17 ] where expression of proinflammatory cytokine and weight are closely related and thus demonstrated that there is a correlation between weight reduction and expression decrease of inflammatory cytokine.…”

“…Conducting medium strength exercises brings positive effects of proinflammatory cytokine decrease, which was expressed from immune cells, and immunity increase in the obese model [ 9 , 10 ] and even with moderate one-off exercise alone, chemotaxis increase of macrophage [ 11 ] and significant results on lymphocyte proliferation response and antigen-specific immune response, which were gained upon the completion of mitogen stimulation with antigen, were achieved [ 12 ], but high-intensity one-off exercise or exhaustive exercise was reported to negatively affect immunity by increasing contagiosity [ 13 ] and proinflammatory cytokine such as IL-6 and IL-1ra and thus the results are contradictory based on exercise intensity or the duration of exercise.…”

In Vivo Evaluation of Immune-Enhancing Activity of Red Gamju Fermented by Probiotic Levilactobacillus brevis KU15154 in Mice

“…In previous studies, most cytokines are known to decrease after a high-intensity exercise [25]. Some prior studies suggest that cytokine gene expression increases during recovery after a high-intensity exercise and/or no difference in the amount of cytokines in the spleen after a high-intensity exercise [26,27]. Thus, the increase in IL-4 and IL-6 mRNA expansion in the spleen, unlike the cytokine in the blood, is thought to change during recovery after a high-intensity exercise.…”

Effects of either aerobic exercise training or quercetin supplementation with a low-fat diet on lipid metabolism, mitochondrial biogenesis, and inflammatory cytokine in skeletal muscle of high fat diet-induced obese mice

“…운동과 관련된 선생연구를 고찰해보면, 고지방 식이로 비 만을 유도한 동물을 이용하여 저지방식이와 운동의 복합처 치는 IL-6와 TNF-α와 같은 염증성 싸이토카인의 발현이 감소 된 것으로 보고하였고 (Chang et al, 2011), 또 다른 연구에서 는 중강도의 유산소 운동이 PGC1-α의 활성도를 증가시켜 전 사인자인 Tfam등의 활성을 증가시키는 것으로 보고하였다 (Benton et al, 2006). (Manach et al, 2004).…”