Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice

Abstract

[Purpose]This study investigated the effect of exercise training and resveratrol supplementation with low fat diet on proinflammatory profiles by Lipopolysaccharide (LPS)-stimulation in peritoneal macrophage of high fat diet mice.[Methods]To accomplish the purpose of this study, C57BL/6 male mice were fed high fat diet (45% fat diet) for 8 weeks. Then these mice were divided into 3 groups; HLC (high fat diet and low fat diet for 8 weeks as the control, n=10), HLR (high fat diet and low fat diet for 8 weeks with resveratrol supplementation, n=10). HLE (high fat diet and low fat diet for 8 weeks with moderate exercise training, n=10). Resveratrol (10 mg/kg) was administrated once a day, 5 days/week for 8 weeks. Exercise training was performed for 8 weeks on a treadmill running for 30-60 min/day at 10-22 m/min, 0% grade, 5 days/week. After exercise training, all the peritoneal macrophage was collected and LPS (0, 0.5, 1.0 μg/ml) were used to stimulate the cells. Then peritoneal macrophage TNF-α, IL-6, MCP-1, IL12p70, IFN-γ, IL-10 were measured by BD cytometric bead array mouse inflammation kit.[Results]As a result, body weight and total cholesterol were significantly reduced in HLE compared with HLC (p<.05). Also, TNF-α and MCP-1 were decreased in HLE compared with HLC (p<.05) by LPS-stimulation (0, 0.5, 1.0 μg/ml) and IL-6, IL-12p70 and IFN-r were decreased in HLE compared with HLC (p<.05) by LPS-stimulation (1.0 μg/ml). But resveratrol supplementation did not affect the result.[Conclusion]These findings suggest that exercise training has beneficial effects on body weight, total cholesterol, peritoneal macrophage and proinflammatory cytokine in high fat diet mice.

NMAAP1 Expressed in BCG-Activated Macrophage Promotes M1 Macrophage Polarization

“…ICR mice (n = 6/group/treatment/experiment) were injected intraperitoneally with 2 mg BCG (Shanghai Institute of Biological Products) or PBS on day 2 and day 10. Peritoneal exudate cells were harvested from these mice, and peritoneal macrophages were purified on days 4, 8, and 12 by washing cells from peritoneal cavity lavage with 5 ml of ice-cold, sterile PBS, according to a previously described protocol ( Park et al, 2014 ). The cells were distributed in DMEM supplemented with 10% FBS in six-well tissue culture plates (5 × 10 6 cells/ml) and incubated for 4 h. Non-adherent cells were removed by washing twice with Opti-MEM at 37°C in a 5% CO 2 incubator.…”

Anti-Nociceptive Effect of Resveratrol During Inflammatory Hyperalgesia via Differential Regulation of pro-Inflammatory Mediators

“…The regulation of pro‐inflammatory cytokines and enzymes by resveratrol is not well documented in hyperalgesia. However, earlier reports show that resveratrol did not affect secretion of pro‐inflammatory cytokines in peritoneal macrophages in different pathological conditions (Park et al, ; Wadsworth and Koop, ).…”

Resveratrol and exercise

“…Similarly, it was reported that RES administration did not boost treadmill stamina over the 12-week training period in mice specifically bred for low-capacity exercise types (47). In contrast to exercise, RES supplementation did not affect peritoneal macrophages in mice (53). An overall evaluation of these results (all of which are animal experiments) indicates that the performance-boosting effects of RES in exercise are inconsistent.…”