Effects of methylprednisolone acetate and glucosamine on proteoglycan production by equine chondrocytes in vitro

Abstract

Methylprednisolone, under noninflammatory conditions present in this study, decreased production of proteoglycan by equine chondrocytes. Glucosamine had a protective effect against inhibition of proteoglycan production at all 3 concentrations studied. This suggested that glucosamine may be useful as an adjunct treatment when an intra-articular injection of a corticosteroid is indicated and that it may be efficacious at concentrations relevant to clinical use.

The use of intra‐articular corticosteroids in the horse: What is known on a scientific basis?

“…MPA. In an in vitro study, Byron et al. (2008) showed a significant decrease in proteoglycans in chondrocytes due to MPA administration and Fubini et al.…”

Influence of Corticosteroids on Interleukin‐1β‐Stimulated Equine Chondrocyte Gene Expression

“…Corticosteroid treatment, however, is not entirely without potential deleterious effects on cartilage metabolism. A number of in vitro and in vivo studies have demonstrated proteoglycan depletion and/or suppression of proteoglycan (GAG) synthesis in equine cartilage exposed to glucocorticoids . Conversely, chondroprotective effects occur in cytokine or lipopolysaccharide‐stimulated cartilage and chondrocyte cultures .…”

Resistance toToxoplasma gondiiInfection in Mice Treated with Silk Protein by Enhanced Immune Responses

“…In a previous study, mice injected with methylprednisolone acetate showed a decrease in circulating CD4 + T-lymphocytes, and enabled the propagation of Cryptosporidium parvum in adult mice [24]. Accordingly, because the effects of methylprednisolone are related to a decrease in T-lymphocyte numbers [24,26,27], it is possible that the proliferation of CD4 + and CD8 + T-cells induced by silk protein confers resistance to the immunosuppressive state induced by methylprednisolone.…”