We propose standardized definitions and criteria for documenting subjective results for clinical orthopedic studies in veterinary medicine. To our knowledge, no common terminology or basis for describing results in this manner has been published. This lack of standardization limits our abilities to communicate study results in a consistent manner, interpret data appropriately, and compare results across studies, centers, and techniques. An initial step toward addressing these deficiencies is to provide clear definitions and criteria for time frames of data collection, subjective outcomes, and complications. These definitions and criteria are recommended for use based on consensus among the authors who have experience and expertise in small animal and equine orthopedic clinical research. Our hope is that this terminology will be implemented so that data collection and reporting are more consistent and effective in veterinary orthopedic reports.
BACKGROUND
Fourier transform-second harmonic generation (FT-SHG) imaging is used as a technique for evaluating collagenase-induced injury in horse tendons. The differences in collagen fiber organization between normal and injured tendon are quantified. Results indicate that the organization of collagen fibers is regularly oriented in normal tendons and randomly organized in injured tendons. This is further supported through the use of additional metrics, in particular, the number of dark (no/minimal signal) and isotropic (no preferred fiber orientation) regions in the images, and the ratio of forward-to-backward second-harmonic intensity. FT-SHG microscopy is also compared with the conventional polarized light microscopy and is shown to be more sensitive to assessing injured tendons than the latter. Moreover, sample preparation artifacts that affect the quantitative evaluation of collagen fiber organization can be circumvented by using FT-SHG microscopy. The technique has potential as an assessment tool for evaluating the impact of various injuries that affect collagen fiber organization.
FGF-2 treatment of bone marrow-derived MSC monolayers enhanced subsequent chondrogenic differentiation in a 3-dimensional culture. This result is important for tissue engineering strategies dependent on MSC expansion for cartilage repair.
Results can be used for comparison with data from other institutions and surgical facilities. Additional precautions should be undertaken when arthroscopic surgery involves draft breeds and tibiotarsal joints.
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