Effects of Methylmercury exposure in 3T3-L1 Adipocytes

Vertigan, Theresa; Dunlap, Kriya L.; Reynolds, Arleigh J.; Duffy, Lawrence K.

doi:10.3934/environsci.2017.1.94

Cited by 7 publications

(12 citation statements)

References 16 publications

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“…Barnes and his colleagues investigated the effect of inorganic mercury chloride on adipogenesis and glucose transport [11,12]. Vertigan, et al showed increased VEGF secretion from differentiated cells due to MeHg exposure [13]. Their findings provide a platform for further studying MeHg species as a potential modulator of metabolic processes in adipocytes.…”

Section: Resultsmentioning

confidence: 99%

“…Thus far, Barnes et al and Vertigan, et al have studied the effects on the 3T3 -L1 cells. Vertigan, et al found that MeHg exposure of 100ng/ml is cytotoxic to the 3T3 L1 cells and also increase VEGF secretion during later stages of differentiation [11][12][13]. Studies to observe the effect of MeHg on ROS levels and the effect of theaflavin-3,3'digigallate, or other anti-oxidants would contribute to the knowledge characterizing the system.…”

Section: Adipokines and Anti-inflammatory Agentsmentioning

confidence: 99%

“…The common cell type used for in vitro studies for the phenomenon of adipogenesis and adipokines are the 3T3-L1 cell line, a well-characterized adipocyte line from mice which can acquire an adipocytelike phenotype. During the differentiation process, 3T3-L1 cells can be induced to differentiate under controlled laboratory conditions using a standard differentiation protocol and are widely accepted as a physiologically faithful in vitro representation of adipogenesis [11][12][13]39].…”

Section: Adipokines and Anti-inflammatory Agentsmentioning

confidence: 99%

“…To the best of our knowledge, only one study exists which has examined the effect of organic mercury, MeHg, on the adipocytes, the specialized cells of the adipose tissue. In studying the cytokine Vascular Endothelium Growth Factor (VEGF) expression by 3T3-L1 cells, Vertigan, et al observed that an exposure of 100ng/ml (0.4uM) of MeHg is cytotoxic to adipocytes [13]. Additionally, they found that 0.4uM MeHg exposure elevates the VEGF secretion during later stages of differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Methyl Mercury, Adipokines, 3T3-L1 Cells and Diabetes

Lk¹

2016

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Diabetes is a contributor to morbidity across the globe and is often associated with obesity and metabolic syndrome. In addition to genetic and lifestyle factors, environmental factors such as metals and persistent organic pollutants may increase the severity or lower the threshold of these conditions. Studies are showing an association between these contaminants and both insulin sensitivity and glucose transport. In cell culture, mercury and methyl mercury are toxic to adipocytes and impact the secretion of cytokines and adipokines. We propose a research model using contaminants like methyl mercury on adipocytes to enhance the existing knowledge on the mechanistic influence of adipokines and reactive oxygen species on 3T3-L1 cell functioning. With this enhanced signaling model, anti-inflammatory agents could be tested at the biochemical level and lead to studies in animal models. Prospective model studies on mixtures of contaminants can contribute to better understanding about the development or severity of diabetes.

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Section: Resultsmentioning

confidence: 99%

Section: Adipokines and Anti-inflammatory Agentsmentioning

confidence: 99%

Section: Adipokines and Anti-inflammatory Agentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Methyl Mercury, Adipokines, 3T3-L1 Cells and Diabetes

Lk¹

2016

ACT

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“…To the best of our knowledge, only three studies have investigated this problematic. It has been shown that MeHg caused an alteration of structure and endocrine function in 3T3-L1 cell line (Vertigan et al, 2015;Chauhan et al, 2019). On the other hand, MeHg did not affect lipid accumulation in human preadipocytes and mesenchymal stem cells up to 100 nM (van den Dungen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Methylmercury displays pro-adipogenic properties in rainbow trout preadipocytes

et al. 2021

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h i g h l i g h t s MeHg induced a dose-dependent accumulation of neutral lipids in preadipocytes. Accumulation of lipids persisted beyond the cellular exposure to MeHg. MeHg increased n-3 PUFA levels in lipid droplets and dropped AA levels in membranes. Expression of adipocyte-and lipid-specific genes was modulated by MeHg. Total mercury accumulated in cells was released when MeHg exposure stopped.

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