Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Santo, Mariana Carolina Di; Alaimo, Agustina; Rubio, Ana Paula Domínguez; Matteo, Regina De; Pérez, Oscar E.

doi:10.1016/j.bbrep.2020.100842

Cited by 6 publications

(2 citation statements)

References 73 publications

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“…73 As shown in Figure 12a, the BC and BC/MoS 2 -CS membranes showed normal cell proliferation (and thus no toxicity against L929 cells), BC/CS promoted cell proliferation, while BC/MoS 2 was marginally cytotoxic: in particular, after 24 h incubation with extracts of the BC, BC/CS, BC/ MoS 2 , and BC/MoS 2 -CS membranes, L929 cell viability was 103, 126, 72, and 99%, respectively. The ability for CS to promote mammalian cell growth has been noted previously in the literature 74 and likely serves to counter any cytotoxicity of MoS 2 in the BC/MoS 2 -CS composite. Furthermore, fluorescence imaging (LIVE/DEAD stain) of the L929 cells cultured on the extracts for 24 h is shown in Figure 12b.…”

Section: 33mentioning

confidence: 55%

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS₂-Chitosan Nanocomposite Materials with Visible Light Illumination

Shen

Jiang

et al. 2021

ACS Appl. Mater. Interfaces

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Owing to the rise in prevalence of multidrugresistant pathogens attributed to the overuse of antibiotics, infectious diseases caused by the transmission of microbes from contaminated surfaces to new hosts are an ever-increasing threat to public health. Thus, novel materials that can stem this crisis, while also functioning via multiple antimicrobial mechanisms so that pathogens are unable to develop resistance to them, are in urgent need. Toward this goal, in this work, we developed in situ grown bacterial cellulose/MoS 2 -chitosan nanocomposite materials (termed BC/MoS 2 -CS) that utilize synergistic membrane disruption and photodynamic and photothermal antibacterial activities to achieve more efficient bactericidal activity. The BC/ MoS 2 -CS nanocomposite exhibited excellent antibacterial efficacy, achieving 99.998% (4.7 log units) and 99.988% (3.9 log units) photoinactivation of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, respectively, under visible-light illumination (xenon lamp, 500 W, λ ≥ 420 nm, and 30 min). Mechanistic studies revealed that the use of cationic chitosan likely facilitated bacterial membrane disruption and/or permeability, with hyperthermia (photothermal) and reactive oxygen species (photodynamic) leading to synergistic pathogen inactivation upon visiblelight illumination. No mammalian cell cytotoxicity was observed for the BC/MoS 2 -CS membrane, suggesting that such composite nanomaterials are attractive as functional materials for infection control applications.

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Section: 33mentioning

confidence: 55%

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS₂-Chitosan Nanocomposite Materials with Visible Light Illumination

Shen

Jiang

et al. 2021

ACS Appl. Mater. Interfaces

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“…Among organic macromolecular-based drug carriers, polymeric NPs have been widely chosen as drug delivery vectors due to their offered advantages: firstly, chemical and physical stability under physiological environments and storage conditions; secondly, excellent biocompatibility and biodegradability, low toxicity, and immunogenicity; thirdly, surface modification with ligands; and finally ease of production [ 75 ]. Depending on the nature of the polymer, various substances such as macromolecules and hydrophobic drugs can be delivered to cells and specialized organs.…”

Section: Different Types Of Nanoformulations For the Delivery Of Poly...mentioning

confidence: 99%

Research progress of polyphenols in nanoformulations for antibacterial application

Dong

Wang

et al. 2023

Materials Today Bio

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Impact of chitosan-based nanocarriers on cytoskeleton dynamics: Current status and challenges

Santo¹,

Alaimo²,

RUBIO³

et al. 2022

Biocell

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Chitosan-based nanocarriers (CS-NCs) show a promising role in improving drugs and bioactive compounds delivery for therapy. However, the effects exerted by CS-NCs at the cellular level, including their recognition and uptake, have not been fully investigated yet. Many factors, including size, shape, concentration, and surface chemistry of CS-NCs, play an important role in determining the types of intracellular signals triggered. The mechanism of uptake and the involvement of the cytoskeleton during the CS-NCs endocytosis variates among the different cell types as well as further effects observed inside cells. In the present work, we discuss the effects induced by CS-NCs per se on the cytoskeleton, a key component in cell architecture and physiology. The focus of this report is made on tumoral and normal biological models in which CS-NCs could differentially affect the cell cytoskeleton. The recent years reports regarding the impact of CS-NCs on cytoskeleton dynamics and the current techniques for its evaluation are summarized and discussed. Understanding mechanisms underlying cytoskeletal impact after cell exposure to CS-NCs is critical for the design of safest value-added formulations in the biomedical field. Furthermore, this revision points out some interesting aspects of cytoskeletal changes and cell death encompassing anti-tumoral effects.

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Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Cited by 6 publications

References 73 publications

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS₂-Chitosan Nanocomposite Materials with Visible Light Illumination

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS₂-Chitosan Nanocomposite Materials with Visible Light Illumination

Research progress of polyphenols in nanoformulations for antibacterial application

Impact of chitosan-based nanocarriers on cytoskeleton dynamics: Current status and challenges

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Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Cited by 6 publications

References 73 publications

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS2-Chitosan Nanocomposite Materials with Visible Light Illumination

Research progress of polyphenols in nanoformulations for antibacterial application

Impact of chitosan-based nanocarriers on cytoskeleton dynamics: Current status and challenges

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Product

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Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS₂-Chitosan Nanocomposite Materials with Visible Light Illumination

Synergistic Photodynamic and Photothermal Antibacterial Activity of In Situ Grown Bacterial Cellulose/MoS₂-Chitosan Nanocomposite Materials with Visible Light Illumination