Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Chaichana, Natthiya; Dheeranupattana, Srisulak

doi:10.7763/ijbbb.2012.v2.89

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…This all causes the induction of ROS bursting. This whole process is lead to the production of secondary metabolites [42] Silybum marianun hairy root culture are able to generate all flavonolignans that are found in the part of fruit but also found in the seeds and leave, while in dried fruit the production was generally lower [34,[43][44][45][46][47] This study is in agreement with our results as in our study fresh extract was used to check on HPLC that showed production of flavonolignans in leaves of the S. marianum plant [48][49][50][51][52][53].…”

Section: Standardsupporting

confidence: 85%

Enrichment of Therapeutically Significant Flavonolignans of Silybum Marianum in Vegetative Parts by Applying Fungal Elicitors, Methyl Jasmonate and Silver Nanoparticles as Elicitor in Hydroponic Culture

Mubeen

Ali

Hasnain

et al. 2021

JPRI

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Introduction: Medicinal plants are being used to treat several diseases for many decades and this is an ancient method to treat the patients. Herbal plant Silybum marianum found most effective one to cure liver disorders. This plant produces silymarin which is a secondary metabolite and have hepatoprotective properties. Silymarin is a mixture of flavonolignans (silybin A, silybin B, isosilybin A isosilybin B, silychristin, silydianin apigenin 7-D glucose and taxifolin) that has antiviral, antibacterial, antifungal and antiallergenic properties. Therefore, the excessive production of silymarin is necessary to cure different types liver disorders, so the present study executed to boost up production of Silymarin flavonolignans in vegetative parts of the Silybum marianum plant by using different elicitors. Materials and Methods: In the present study, elicitation technique in hydroponics system was used to enhance the production of pharmacologically active flavonolignans in Silybum marianum. Fungal elicitors prepared from lyophilized Aspergillus niger biomass, methyl jasmonate, silver nanoparticles and combination of silver nanoparticles and methyl jasmonate were added (0.2g/l), (100µM/l), (1 ppm) and (100µM/lppm) in hydroponics with Hoagland’s solution in hydroponics to enhance the production of flavonolignans of Silybum marianum. Controls were also set for each treatment. Plants were harvested after 72 hours of introduction of elicitors. High performance liquid chromatography technique was used for analytical purpose. Four solvents (methanol, acetonitrile, chloroform and 2% Trifloro acetic acid) were used in HPLC. Column was C18 and run time of sample was 1 hour. Silybum marianum’s seed extract was used as a standard. Extract of control and treated plants were run on the same polarity in HPLC. Results: Results showed that after elicitation significant increase was observed in production of silymarin’s flavonolignans (silybin A, silybin B, isosilybin A, isosilybin B and apigenin 7-D glucose) in vegetative part of the plant but rate of production was different for each elicitor, fungal elicitors prepared from lyophilized Aspergillus niger biomass proved best among all treatments.

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Section: Standardsupporting

confidence: 85%

Enrichment of Therapeutically Significant Flavonolignans of Silybum Marianum in Vegetative Parts by Applying Fungal Elicitors, Methyl Jasmonate and Silver Nanoparticles as Elicitor in Hydroponic Culture

Mubeen

Ali

Hasnain

et al. 2021

JPRI

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“…The similar study showed that production of stemofoline alkaloids enhanced 1.42fold in root extracts of Stemona sp. treated with 1.0 MeJa, when compared with control plants [31]. MeJa treatment induced the accumulation of dihydrosanguinarine alkaloid about 1.5-fold higher than in control [32].…”

Section: Influences Of Meja and Put On Tryptanthrin And Indirubin Productionmentioning

confidence: 83%

Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı

Karakaş

2019

International Journal of Secondary Metabolite

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Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's. In this study, we investigated the influences of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in vitro. In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase. The highest increase in tryptanthrin production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control (untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin decreased about 2.56-fold in the leaves of plantlets treated with Put, when compared to control. The highest indirubin production was obtained in the leaves of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg g-1) but treatments with Put didn't show any positive affect on the indirubin production. Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).

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“…The insignificant increased effect of SA on the biomass obtained in our results confirm those of Kang et al (2004) in the adventitious roots of Scopolia parviflora. Chaichana et al (2012) reported that, in general, SA elicitation has a negative effect on growth. In an earlier study, it was reported that SA treatment slightly inhibited the growth of Salvia miltiorrhiza cell cultures (Li et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of polyploidization and elicitation on biomass and hyoscyamine content in hairy roots of Datura stramonium

Belabbassi¹,

Khelifi-Slaoui²,

Zaoui³

et al. 2016

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Description of the subject. The hyoscyamine, a tropane alkaloid, widely used in medicine, can be produced from Datura sp. (Solanaceae). However, its content in the spontaneous roots remains low; therefore, hairy roots (HRs) were envisaged as a potential alternative to improve its biosynthesis. The hairy roots are characterized by a good genetic stability and a rapid growth. Indeed, Datura stramonium HRs have widely been studied in the perspective of improving the yield of hyoscyamine. This study is part of this same perspective. Objectives. This paper aims to study the effects of polyploidization of HRs induced by colchicine in synergy with elicitation (with acetylsalicylic [ASA] or salicylic acids [SA]) on the hyoscyamine content in D. stramonium. Method. Colchicine was applied at different concentrations and periods, on a selected hairy root line (LDS) of D. stramonium obtained by infection with Agrobactrium rhizogenes strain A4. The selection of tetraploid HR lines was performed by the cytogenetic analysis using light microscopy. The effect of polyploidization and elicitation was studied on the biomass (dry weight) and hyoscyamine content of HRs. Results. The untreated HR line (control) shows a diploid level with 2n = 24 chromosomes. However, the HR lines treated with colchicine show, in most cases, an endoreduplication of their genetic material. The survival rate of endoreduplicated lines varies between 30% and 93%, depending on concentration and exposure time to colchicine. Moreover, the tetraploid HR line shows an increase in its biomass and hyoscyamine content in comparison to the diploid HR line (LDS). Further, elicitation of HRs by ASA or AS at the 10-4 M concentration causes a low decrease or increase in dry weight, respectively. However, the same treatments show a significant increase in the yield of hyoscyamine in elicited HR lines. Consequently, our work indicates that the combination of polyploidy and elicitation can lead to significant improvements in hyoscyamine biosynthesis and content due to their synergistic effects. Conclusions. Elicitation of tetraploid hairy root lines improves significantly their content of hyoscyamine.

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Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Cited by 11 publications

References 27 publications

Enrichment of Therapeutically Significant Flavonolignans of Silybum Marianum in Vegetative Parts by Applying Fungal Elicitors, Methyl Jasmonate and Silver Nanoparticles as Elicitor in Hydroponic Culture

Enrichment of Therapeutically Significant Flavonolignans of Silybum Marianum in Vegetative Parts by Applying Fungal Elicitors, Methyl Jasmonate and Silver Nanoparticles as Elicitor in Hydroponic Culture

Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı

Synergistic effects of polyploidization and elicitation on biomass and hyoscyamine content in hairy roots of Datura stramonium

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