Effects of methionine hydroxyl analog chelated zinc on laying performance, eggshell quality, eggshell mineral deposition, and activities of Zn-containing enzymes in aged laying hens

Min, Yuna; Liu, F X; Qi, Xiantao; Ji, Shengwei; X, S; Liu, X; Wang, Z P; Gao, Yujie

doi:10.3382/ps/pey203

Cited by 32 publications

(22 citation statements)

References 35 publications

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“…Zn is necessary for maintenance of structural and functional integrity of biological membranes, facilitation of gene expression and protein synthesis, enzyme structure and function, appetite regulation and good food utilisation (10,11) . Zn is closely linked to eggshell formation (6,12,13) . Especially, Zn plays an important role as a cofactor of carbonic anhydrase (CA) which involves in catalysing hydration of metabolic CO 2 to HCO À 3 and supplying carbonate ions during eggshell formation (4) .…”

mentioning

confidence: 99%

Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens

Miao

Zhu

et al. 2019

Br J Nutr

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The present study explored the mechanism of Zn-methionine (Zn-Met) influencing eggshell quality of laying hens and investigated whether the mechanism was related to Ca deposition. Hyline grey layers (n 384, 38 weeks old) were divided into four groups: 0 mg Zn/kg, 40, 80 mg Zn/kg as Zn-Met, and 80 mg Zn/kg as zinc sulphate (ZnSO4). Eggshell quality, Zn contents, Zn-containing enzyme activities and expressions of shell matrix proteins in eggshell gland (ESG) were analysed. Zn-Met treatment at 80 mg/kg increased (P < 0·05) egg weight and eggshell strength throughout the experiments. The 80 mg/kg Zn-Met group (P < 0·05) had decreased mammillary knob width and larger relative atomic weight percentage of Ca, stronger signal intensity of Ca in linear distribution and the Ca was more evenly distributed in the transversal surface of eggshell. Zn contents (P < 0·001) in yolk and serum, Ca, albumin (Alb) levels in ESG as well as carbonic anhydrase (CA) activity in serum (P < 0·05) and mRNA levels of CA and Ca-binding protein-d28k (CaBP-D28k) (P < 0·001) in the 80 mg/kg Zn-Met group were the highest among all treatments. In conclusion, shell strength as one of eggshell qualities was mostly related to mammillary cone width in ultrastructure caused by the pattern of Ca deposition in eggshell formation. Also, the increase in Zn-Met-induced Ca deposition may be due to the increased Zn contents in serum and tissues, which were attributable to the increased CA concentrations in serum, Ca, Alb levels and up-regulated CA and CaBP-D28k mRNA levels in ESG.

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confidence: 99%

Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens

Miao

Zhu

et al. 2019

Br J Nutr

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“…In this research, organic zinc supplement significantly improved total protein and globulin. Previous studies also proved that dietary Zn supplementation significantly affected serum total protein, albumin, glucose, immunoglobulin, and the performance of laying hens [9,28,29]. Urea nitrogen is a product of protein degradation in the body.…”

Section: Discussionmentioning

confidence: 99%

“…When broilers suffered coccidiosis vaccination, organic trace mineral complexes supplied in diets improved the immune response and lowered lesion scores [8]. Our team’s previous study also showed that 40 and 80 mg/kg methionine hydroxyl analogue chelated zinc (MHA-Zn) supplement increased eggshell quality and promoted trace element deposit [9,10]. Therefore, significant differences exist between inorganic zinc and organic zinc in the body’s antioxidant capacity, but further study is needed to clarify the different mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Methionine Hydroxyl Analogue Chelate Zinc on Antioxidant Capacity and Liver Metabolism Using 1H-NMR-Based Metabolomics in Aged Laying Hens

Xing

et al. 2019

Animals

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Simple SummaryZinc, an essential trace element for laying hens, plays an important role in biological processes, such as growth, tissue growth and repairment, skeletal development, and immune competence, which also has better effects on growth performance, biochemical indexes, and antioxidant capacity. Our previous work has shown that methionine hydroxyl analogue chelated zinc (MHA-Zn) has better effects on eggshell quality, the apparent retention of minerals and nutrients, trace element deposit, and metallothionein (MT) mRNA expression. The objective of the current study was to investigate the effects of different levels of MHA-Zn on antioxidant capacity and liver metabolism of aged laying hens. The results suggest that dietary supplementation of MHA-Zn levels at 80 mg/kg has better effects on antioxidant capacity and liver metabolism, as well as homeostasis of the body. AbstractThis study aimed to investigate the effects of different levels of methionine hydroxyl analogue chelated zinc (MHA-Zn) on antioxidant capacity and liver metabolism of aged laying hens. A total of 960 57-week-old layers were fed a basal diet (Zn: 35.08 mg/kg) without extra zinc for two weeks, and then allocated to four treatments consisting of eight replicates of 30 birds each for 14 weeks. Four levels of Zn (zinc sulfate (ZnSO4): 80 mg/kg; MHA-Zn: 20, 40, 80 mg/kg) were added to the diet. The results indicated that compared with inorganic zinc, organic zinc of 80 mg/kg has a significant advantage in improving the antioxidant capacity of aged hens, which increased the level of Cu/Zn-superoxide dismutase (SOD) and the total antioxidant capacity (T-AOC) in the serum and liver, and reduced the concentration of malondialdehyde (MDA) of laying hens. The serum albumen composition was significantly modified, meanwhile, the level of total protein, globulin, and urea increased remarkably, whereas serum glutamic-oxaloacetic transaminase decreased notably in 80 mg/kg MHA-Zn groups. Compared with the 20 mg/kg MHA-Zn group, the metabolic profile of 40 and 80 mg/kg MHA-Zn groups was higher than that of the inorganic zinc group. Furthermore, integrated key metabolic pathway analysis showed that 40 and 80 mg/kg MHA-Zn groups participated in the regulation of glutathione metabolism, glycine, serine, and threonine metabolism. Therefore, this study suggests that 40 and 80 mg/kg supplementation of MHA-Zn can increase the activity of Cu/Zn-SOD and T-AOC and decrease MDA; additionally the 80 mg/kg MHA-Zn group has better antioxidant capacity. Meanwhile, the enhanced MHA-Zn promoted methionine (Met) synthesis and protein metabolism.

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“…From the literature search a total of nine studies were identified. These studies were performed in pigs (Liu et al., 2014), chickens for fattening (Yuan et al., 2011; Zhao et al., 2016), laying hens (Manangi et al., 2015; Min et al., 2018), dairy cows (Nemec et al., 2012), heifers (Whitehurst et al., 2014), fish (Savolainen and Gatlin, 2010) and shrimp (Katya et al., 2016). No adverse effects were reported.…”

Section: Assessmentmentioning

confidence: 99%

Assessment of the application for renewal of authorisation of zinc chelate of hydroxy analogue of methionine for all animal species

Bampidis¹,

Azimonti²,

Bastos³

et al. 2020

EFS2

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The Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the application for renewal of authorisation of zinc chelate of hydroxy analogue of methionine (Mintrex®Zn) for all animal species. The FEEDAP Panel has delivered three opinions (during 2008 and 2009) on the safety and efficacy of the additive. The additive was authorised in 2010 as ‘Zinc chelate of hydroxy analogue of methionine’ containing 17.5−18 % zinc, 81 % (2‐hydroxy‐4‐methylthio)butanoic acid (dl‐methionine hydroxy analogue, HMTBa) and maximum 1% mineral oil. Following some modifications in the manufacturing process, the additive does not contain mineral oil and the applicant proposes the following specifications: ≥ 17 % zinc and ≥ 79 % HMTBa. The data provided indicate that the additive complies with the new specifications. No new evidence was found that would make the FEEDAP Panel reconsidering its previous conclusions on the safety for target species, consumers and environment. The applicant provided new studies on the effects of the additive on the respiratory tract and on skin and eyes. Data on the characterisation of the additive and the new studies on skin/eyes led the Panel to reconsider the safety for the user. Owing to the zinc and nickel content of Mintrex®Zn, the handling of the additive poses a risk to users by inhalation; the additive is not a skin or eye irritant but is considered a skin sensitiser. The present application did not include a proposal for amending or supplementing the conditions of the original authorisation that would have an impact on the efficacy of the additive; therefore, there was no need for assessing the efficacy of the additive in the context of the renewal of the authorisation.

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Effects of methionine hydroxyl analog chelated zinc on laying performance, eggshell quality, eggshell mineral deposition, and activities of Zn-containing enzymes in aged laying hens

Cited by 32 publications

References 35 publications

Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens

Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens

Effects of the Methionine Hydroxyl Analogue Chelate Zinc on Antioxidant Capacity and Liver Metabolism Using 1H-NMR-Based Metabolomics in Aged Laying Hens

Assessment of the application for renewal of authorisation of zinc chelate of hydroxy analogue of methionine for all animal species

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