Effects of methanol extract of Cirsium japonicum var. ussuriense and its principle, hispidulin‐7‐O‐neohesperidoside on hepatic alcohol‐metabolizing enzymes and lipid peroxidation in ethanol‐treated rats

Abstract

Effects of the methanol extract of Cirsium japonicum var. ussuriense and hispidulin 7-O-neohesperidoside isolated from the plant on hepatic alcohol-metabolizing enzymes and lipid peroxidation were studied in rats treated with ethanol. Rats treated with 10% alcohol solution for 6 weeks were orally administered with 250 or 500 mg of methanol extract or 10 or 20 mg of hispidulin 7-O-neohesperidoside per kg body weight daily during the last week of ethanol treatment. The administration of the methanol extract of herbal plant and hispidulin 7-O-neohesperidoside in ethanol-treated rats significantly enhanced the activities of hepatic alcohol dehydrogenase, microsomal ethanol-oxidizing system and aldehyde dehydrogenase in a dose-dependent manner. The extract and the compound decreased hepatic lipid peroxidation along with an increase in hepatic content of reduced glutathione. The methanol extract and hispidulin 7-O-neohesperidoside of C. japonicum var. ussuriense also increased the activity of glutathione reductase, but had no effect on gamma-glutamylcysteine synthase. The results suggest that C. japonicum var. ussuriense may alleviate alcoholic toxicity by enhancing ethanol oxidation as well as inhibiting lipid peroxidation, and hispidulin 7-O-neohesperidoside is one of the active substances responsible for the protective effects of this plant.

Pretreatment of albino rats with aqueous leaf extract of <i>Ziziphus mauritiana</i> protects against alcohol-induced liver damage

“…Chronic alcohol ingestion has been reported to result in increased lipid peroxidation through the formation of 4-hydroxy 2,3 neonenal, 4-hydroxy 2-3-alkanals and malondialdehyde 22 . Increased lipid peroxidation could be a direct consequence of decreased hepatic reduced glutathione content, which gives rise to an increase in malondialdehyde formation 8 . Escalation of lipid peroxidation in the liver due to chronic alcohol ingestion may be the result of increased formation of free radicals as well as the inhibition of antioxidant enzymes such as superoxide dismutase and catalase 28 .…”

“…The most important characteristic of toxic free radicals either in vivo or in vitro is peroxidation of lipids resulting in tissue damage and death of affected cells 6 . Several reports have implicated free radical-induced lipid peroxidation in the pathogenesis of alcoholinduced liver toxicity 7,8 . Antioxidants play an important role in the protection of cells and tissues against free radical-mediated tissue injury 9 .…”

Polyacetylene Compound from Cirsium japonicum var. ussuriense Inhibits the LPS-Induced Inflammatory Reaction via Suppression of NF-κB Activity in RAW 264.7 Cells

“…Linarin, luteolin, acacetin etc. from the Compositae family has been reported to have various biological activities including anti-oxidant activity (Park et al, 2004;Jeong et al, 2008). However, no report has been issued on anti-infl ammatory activity or mode of action by 1-Heptadecene-11, 13-diyne-8, 9, 10-triol (PA) from the root of Cirsium japonicum var.…”

Determination of pectolinarin inCirsiumspp. using HPLC/UV analysis

“…Cirsium spp. is also bioactive and has antimicrobial, anti-diabetic, anti-oxidant, hepatoprotective, antiinflammatory, vasorelaxant, and anti-cancer properties (Perez et al 2001;Park et al 2004;Nazaruk and Jakoniuk 2005;Liu et al 2007;Jeong et al 2008;Kim et al 2008;Ku et al 2008;Lim et al 2008;Yoo et al 2008).…”