Effects of metabolic substrates and ionic environment on in-vitro activation of delayed implanting mouse blastocysts

Nieder, Gary L.; Weitlauf, Harry M.

doi:10.1530/jrf.0.0730151

Cited by 12 publications

(7 citation statements)

References 18 publications

(28 reference statements)

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“…During delayed implantation, the blastocyst is metabolically dormant and incompetent to initiate attachment in the uterus (Nieder and Weitlauf, 1985; Van Blerkom et al, 1978). Although embryos develop into blastocysts and undergo zona dissolution, they show signs of being inactive with subnormal metabolic activity, reduced cell divisions with low DNA synthesis (Lopes et al, 2004).…”

Section: Embryonic Preparation For Implantation Necessitates “Blasmentioning

confidence: 99%

“…Several in vitro experiments support this view. For instance, depletion of glucose and/or arginine and leucine from a conventional medium impedes blastocyst growth (Nieder and Weitlauf, 1985). Although these observations are valuable for the clues about blastocyst activation, the underlying molecular and cellular mechanisms are still unknown.…”

Section: Embryonic Preparation For Implantation Necessitates “Blasmentioning

confidence: 99%

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Physiological and molecular determinants of embryo implantation

Zhang

Lin

Kong

et al. 2013

Molecular Aspects of Medicine

439

480

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Embryo implantation involves the intimate interaction between an implantation-competent blastocyst and a receptive uterus, which occurs in a limited time period known as the window of implantation. Emerging evidence shows that defects originating during embryo implantation induce ripple effects with adverse consequences on later gestation events, highlighting the significance of this event for pregnancy success. Although a multitude of cellular events and molecular pathways involved in embryo-uterine crosstalk during implantation have been identified through gene expression studies and genetically engineered mouse models, a comprehensive understanding of the nature of embryo implantation is still missing. This review focuses on recent progress with particular attention to physiological and molecular determinants of blastocyst activation, uterine receptivity, blastocyst attachment and uterine decidualization. A better understanding of underlying mechanisms governing embryo implantation should generate new strategies to rectify implantation failure and improve pregnancy rates in women.

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Section: Embryonic Preparation For Implantation Necessitates “Blasmentioning

confidence: 99%

Section: Embryonic Preparation For Implantation Necessitates “Blasmentioning

confidence: 99%

Physiological and molecular determinants of embryo implantation

Zhang

Lin

Kong

et al. 2013

Molecular Aspects of Medicine

439

480

View full text Add to dashboard Cite

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“…Embryonic dormancy in DNA synthesis associated with delayed implantation is unlikely caused by shortage of factors necessary to continually develop but likely caused by the presence of inhibitory factor of protein nature in the uterine luminal fluid [3,9].…”

Section: Methodsmentioning

confidence: 99%

“…The culture droplet was prepared on a hydrophobic membrane filter (Gelman Sciences, Ann Artor, U.S.A. H]thymidine incorporation was assessed by the method described previously [9] with a modification as below. Blastocysts were incubated in 50 µl of DMEM/F-12 containing 5.0 mCi/ml methyl-[ 3 H]thymidine (50-80 Ci/ mmol, Amersham, Arlington Heights, U.S.A.) for 1 hr at 37°C.…”

Section: Methodsmentioning

confidence: 99%

A Delayed-Implantation-Associated Protein Prevents Resumption of DNA Synthesis by the Trophectoderm of Delayed-Implanting Mouse Blastocysts in vitro.

Katagiri

Takahashi

Kanagawa

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. Objective of this study was to determine the ability of a delayed-implantation-associated protein (MW 170,000, DIAP170K) to inhibit DNA synthesis by mouse blastocysts. Mice were ovariectomized on day 3 of pregnancy and treated with daily injections with 1 mg progesterone till day 7 to induce delayed implantation. Blastocysts were collected on day 8 with or without a single injection of 25 ng estradiol-17β on day 7 that activates blastocyst metabolisms (activated blastocysts and delayed-implanting blastocysts respectively). DNA synthesis was determined by measuring [ 3 H]thymidine incorportion by blastocysts. DIAP170K at 10 µg/ml suppressed resumption of DNA synthesis by delayed-implanting blastocysts and suppression was maximal at 50 µg/ml. However, DIAP170K did not affect DNA synthesis by blastocysts obtained on day 5 of pregnancy (normal blastocysts) and activated blastocysts. Resumption of DNA synthesis in the inner cell mass (ICM) and trophectoderm from delayed-implanting blastocysts was then separately assessed. DNA synthesis resumed in the trophectoderm of intact blastocysts during 24-hr culture but not in the trophectoderm cultured apart from the ICM. DIAP170K inhibited the resumption of DNA synthesis by the trophectoderm of intact delayed-implanting blastocysts but did not affect DNA synthesis by the ICM. In conclusion, DIAP170K inhibits resumption of DNA synthesis by trophectoderm of delayed-implanting blastocysts. This action of DIAP170K may play a central role in maintaining, but not achieving, dormancy of DNA synthesis by delayed-implanting blastocysts in mice. MATERIALS AND METHODSBlastocysts: Virgin 8-12 week-old CD1 female mice (Animal Care Centre, University of British Columbia, Vancouver, Canada) were housed with fertile males of the same strain overnight on the day of estrus. Females with a vaginal plug on the following morning were considered pregnant (day 1). Females were randomly divided into 3 groups. Mice in the first group were bilaterally ovariectomized on day 3 and given daily injections (s.c.) with 1 mg progesterone (Sigma, St. Louis, U.S.A.) prepared in 0.05 ml sesame oil until day 7. Blastocysts were collected on day 8 (delayed-implanting blastocysts). Mice in the second group were ovariectomized, followed by daily progesterone injections as above plus a single injection (s.c.) of 25 ng estradiol-17β (Sigma) in 0.05 ml sesame oil on day 7. Blastocysts were collected on day 8 (activated blastocysts). Mice in the last group were intact and blastocysts were collected on day 5 (normal blastocysts).DIAP170K preparation: DIAP170K was obtained from mouse uteri during experimentally induced delayed implantation [7]. Partially purified DIAP170K used in this study showed one broad band (>97% of total protein by HPLC analysis) and a few trace bands on SDS-PAGE under reduced and unreduced conditions. The apparent molecular weight of the main band was estimated as 168,500 on SDS-PAGE.Blastocyst culture: Blastocysts were cultured in DNA synthesis as well as other metabolic events in mou...

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“…1 and 2). A cytotoxic factor with an apparent molecular weight of 44,000 was detected in the uterine extract from OVX-P mice (fractions [28][29] and Day-5 pregnant mice (fractions 28-30) but not in the sera (Figs. 1 and 2).…”

Section: Separation Of Proteinmentioning

confidence: 96%