Effects of medetomidine on hepatic EROD activity in three species of fish

Lennquist, Anna; Celander, Malin; Förlin, Lars

doi:10.1016/j.ecoenv.2007.05.004

Cited by 20 publications

(7 citation statements)

References 28 publications

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“…Induction of CYP protein synthesis has been reported in some organisms, and a coplanar conformational structure is identified as important for the induction of CYP1A (Murray et al,1992; Kobayashi et al,1993). Furthermore, other previous studies indicated that EROD could be induced in fish exposed to some chemical contaminants (Egaas et al,1999; Jonsson et al,2006; Lennquist et al,2008). In this study, induced hepatic EROD activity in the fish exposed to PCZ was significantly higher than the control group, indicating that PCZ had toxic effects through binding to the aryl hydrocarbon receptor (AhR).…”

Section: Discussionmentioning

confidence: 87%

Multiple biomarkers responses in juvenile rainbow trout, Oncorhynchus mykiss, after acute exposure to a fungicide propiconazole

Žlábek

Velíšek

et al. 2011

Environmental Toxicology

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In this study, the toxic effects of PCZ, a triazole fungicide present in aquatic environment, were studied in rainbow trout, Oncorhynchus mykiss, by an acute toxicity test. Compared to the control group, fish exposed to PCZ (96-h-LC50 , 5.04 mg/l) showed significantly higher (P < 0.05) plasma NH 3 and GLU concentration and the activities of plasma enzymes including CK, ALT, AST, LDH, but the TP content was not significantly different (P > 0.05). The oxidative stress indices (levels of LPO and CP) of brain and muscle in the experimental group were higher compared to the control group, especially for a significant change (P < 0.05) in the brain. SOD, CAT, GPx and GR activity in the brain of experimental groups was significantly lower (P < 0.05), however, an opposite tendency was found out in muscle. In addition, there are significant correlations between TBARS and CAT, TBARS and GPx, CP, and CAT, GR, and GPx in the fish brain. Thus, PCZ exposure changed the oxidative stress indices and plasma characteristics, and these changes may be used as potential bioindicators of the exposure and effect of PCZ in the controlled experiment. The use in monitoring of PCZ exposure under natural field conditions is possible, but it needs further investigations. List of abbreviations PCZ = propiconazole; rOs = reactive oxygen species; LPO = lipid peroxidation; cP = carbonyl protein; sOD = superoxide dismutase; cAt = catalase; GPx = glutathione peroxidase; Gr = glutathione reductase; tbArs = thiobarbituric acid-reactive substances; DMsO = dimethyl sulphoxide; GssG = oxidized glu-tathione; tcA = trichloroacetic acid; bHt = butylated hydroxytoluene; DNPH = 2,4-di-nitrophenylhydra-zine; EDtA = ethylenediamine-tetraacetic acid; NADPH = nicotinamide adenine dinucleotide phosphate; HUFA = highly unsaturated fatty acids; NH 3 = ammonia; GLU = glucose; tP = total proteins; cK = creatine kinase; LDH = lactate dehydrogenase; ALt = alanine aminotransferase; Ast = aspartate aminotransferase

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Section: Discussionmentioning

confidence: 87%

Multiple biomarkers responses in juvenile rainbow trout, Oncorhynchus mykiss, after acute exposure to a fungicide propiconazole

Žlábek

Velíšek

et al. 2011

Environmental Toxicology

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“…The interdisciplinary research program Marine Paint [2], in which our group has been a part, has since 2001 been investigating the substance medetomidine [29] (see Scheme 1) which is based on imidazole for antifouling purposes [30][31][32]. Medetomidine is exceptionally efficient against fouling barnacles [33], which is one of the most problematic foulants in the north Atlantic, yet, without seriously affecting nontarget organisms [34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Imidazole and Triazole Coordination Chemistry for Antifouling Coatings

Trojer

Movahedi

Blanck

et al. 2013

Journal of Chemistry

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Fouling of marine organisms on the hulls of ships is a severe problem for the shipping industry. Many antifouling agents are based on five-membered nitrogen heterocyclic compounds, in particular imidazoles and triazoles. Moreover, imidazole and triazoles are strong ligands for Cu2+and Cu+, which are both potent antifouling agents. In this review, we summarize a decade of work within our groups concerning imidazole and triazole coordination chemistry for antifouling applications with a particular focus on the very potent antifouling agentmedetomidine. The entry starts by providing a detailed theoretical description of the azole-metal coordination chemistry. Some attention will be given to ways to functionalize polymers with azole ligands. Then, the effect of metal coordination in azole-containing polymers with respect to material properties will be discussed. Our work concerning the controlled release of antifouling agents, in particular medetomidine, using azole coordination chemistry will be reviewed. Finally, an outlook will be given describing the potential for tailoring the azole ligand chemistry in polymers with respect to Cu2+adsorption and Cu2+→Cu+reduction for antifouling coatings without added biocides.

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“…In vivo increase Rainbow trout, turbot, Atlantic cod (no effect), Atlantic salmon, three-spined stickleback Lennquist et al, 2008 and unpublished data B. In vitro EROD inhibition Potent inhibitor Rainbow trout, turbot, Atlantic cod Lennquist et al (2008) C. Oxygen consumption Decreased Lumpfish, Atlantic cod (no effect), turbot Bellas et al (2005) (1) Atlantic salmon, from above: 50 nM, 5 nM and control.…”

Section: Erod Activitymentioning

confidence: 91%

“…In vitro, medetomidine was shown to be a potent inhibitor of EROD activity with median inhibition values in the nanomolar range. Studies were performed in microsomes from betanaphthoflavone treated rainbow trout (IC50 35 ± 10 nM), Atlantic cod (IC50 111 ± 70 nM) and turbot (IC50 47 ± 17 nM) (Lennquist et al, 2008). Decreased oxygen consumption was observed in fry from lumpfish and turbot from concentrations from 5-10 nM and exposures up to 96 h. However, this effect was found to be reversible.…”

Section: Erod Activitymentioning

confidence: 98%

“…Increased activity of the hepatic enzyme CYP1A, measured as EROD activity, was observed in rainbow trout and turbot (Lennquist et al, 2008) and in three-spined stickleback and Atlantic salmon (unpublished data) at concentrations from 0.5-100 nM, depending on species and route of administration. The increase varied from 1.5 to 7 times, which is considered to be a weak …”

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confidence: 94%

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