Effects of mechanical abrasion challenge on sound and demineralized dentin surfaces treated with SDF

Abstract

This study evaluated the effect of mechanical abrasion on the surface integrity, color change (ΔE) and antibacterial properties of demineralized and sound dentin surfaces treated with silver-diammine-fluoride (SDF). The dentin specimens were divided into two groups: sound and demineralized dentin, then divided into three sub-groups, control (no-treatment), SDF, and SDF + potassium-iodide (KI). Each sub-group was further divided into two groups, one exposed to mechanical brushing and the other without brushing. Specimens were analyzed for the ΔE, surface roughness/surface loss and antibacterial properties (CFU, optical density and fluorescent microscope). Repeated Measures ANOVA was used for statistical analysis of color change while one-way ANOVA was used for CFU analysis. SDF and SDI + KI groups showed significant reduction in ΔE with brushing in the sound dentin group unlike the demineralized group. The surface roughness values were higher for both SDF and SDF + KI groups but roughness values significantly decreased after brushing. Both SDF and SDF + KI groups revealed significantly less surface loss than control. The SDF group showed high anti-bacterial effect after brushing, unlike SDF + KI group. So, we concluded that mechanical brushing improved the esthetic outcome. While, SDF and SDF + KI could protect the dentin surface integrity. SDF-treated dentin possesses an antibacterial property even after mechanical brushing.

Efficacy of 30% and 38% Silver Diamine Fluoride in Arresting Caries Lesions After Different Application Times: An in Vitro Study

“…These results are corroborated by others [23], who found statistically significant higher pH at the culture medium after cariogenic challenge for SDF treated tooth surfaces compared to control and NaF treated groups, suggesting that F and Ag present in SDF have a strong effect on the inhibition of acid production by Streptococcus mutans, thus inhibiting their metabolic activity. Furthermore, although no in vitro study to date has simulated the oral environment with toothbrushing for 21 days, a recent study evaluated the mechanical effect of brushing on demineralized surfaces treated with 38% SDF and found that after 7 days of brushing, the SDF-treated dentin remained with antibacterial properties [24].…”

Penetration of Silver Diamine Fluoride in Deep Carious Lesions of Human Permanent Teeth: An In Vitro Study

“…The SDF penetration throughout the entire thickness of carious lesions allows the antimicrobial effect, which is mainly derived from the silver element [ 9 , 12 , 36 ], to occur thoroughly. The exposure of reversible denatured collagen results in the precipitation of silver particles within demineralized carious dentin due to the presence of functional groups with a high affinity for silver ions, such as sulfur and nitrogen groups [ 19 , 37 ]. Silver phosphate (Ag 3 PO 4 ), silver chloride (AgCl), and silver protein complexes have been identified as the reaction products of SDF with tooth tissue in previous research [ 38 , 39 ].…”

Effect of Coloring Beverages on Color Stability of Single Shade Restorative Material: An In Vitro Study

“…A spectrophotometer (Vita Easy shade compact, Vita, Zahnfabrik, Bad Sackingen, Germany) was used to measure the color parameters and was expressed using International Commission on Illumination System (CIE L*a*b*) where; L* coordinate representing color luminosity (white to black), a* (green to red), and b* (blue to yellow) both representing the chromaticity of the color. The color changes (ΔE*) were calculated [9], [10] as follows:…”