Effects of MCLR exposure on sex hormone synthesis and reproduction-related genes expression of testis in male Rana nigromaculata

Jia, Xiao‐Bin; Liu, Zhengquan; Lu, Xiang‐Jun; Tang, Juan; Wu, Yan; Du, Qiongxia; He, Jianbo; Zhang, Xinyun; Jiang, Jinxiao; Liu, Wenli; Zheng, Yi; Ding, Ying; Zhu, Weiqin; Zhang, Hangjun

doi:10.1016/j.envpol.2018.01.057

Cited by 26 publications

(20 citation statements)

References 55 publications

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“…The observed significant decrease in estrone levels following exposure with 500 ng/mL MCs/NOD-R mixture or 1000 ng/mL MC-LR supports a possible interaction with 17β-HSD (Fig 2). An effect of MC-LR on the gene expression of 17β -HSD has also been observed in male Rana nigromaculata frogs [67]. The same study reports in addition decreased testosterone and increased estradiol levels following long-term exposure (14 days) with 1 μg/L and 0.1 μg/L MC-LR, respectively.…”

Section: Discussionsupporting

confidence: 56%

Microcystins and Microcystis aeruginosa PCC7806 extracts modulate steroidogenesis differentially in the human H295R adrenal model

et al. 2020

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The aim of this study was to investigate the potential interference of cyanobacterial metabolites, in particular microcystins (MCs), with steroid hormone biosynthesis. Steroid hormones control many fundamental processes in an organism, thus alteration of their tissue concentrations may affect normal homeostasis. We used liquid chromatography–tandem mass spectrometry (LC–MS/MS) to investigate the modulation of 14 hormones involved in the adrenal steroid biosynthesis pathway using forskolin-treated H295R cells, following exposure with either microcystin-LR (MC-LR) alone, a mixture made up of MC-LR together with eight other MCs and nodularin-R (NOD-R), or extracts from the MC-LR-producing Microcystis aeruginosa PCC7806 strain or its MC-deficient mutant PCC7806mcyB−. Production of 17-hydroxypregnenolone and dehydroepiandrosterone (DHEA) was increased in the presence of MC-LR in a dose-dependent manner, indicating an inhibitory effect on 3β-hydroxysteroid dehydrogenase (3β-HSD). This effect was not observed following exposure with a MCs/NOD-R mixture, and thus the effect of MC-LR on 3β-HSD appears to be stronger than for other congeners. Exposure to extracts from both M. aeruginosa PCC7806 and M. aeruginosa PCC7806mcyB− had an opposite effect on 3β-HSD, i.e. concentrations of pregnenolone, 17-hydroxypregnenolone and DHEA were significantly decreased, showing that there are other cyanobacterial metabolites that outcompete the effect of MC-LR, and possibly result instead in net-induction. Another finding was a possible concentration-dependent inhibition of CYP21A2 or CYP11β1, which catalyse oxidation reactions leading to cortisol and cortisone, by MC-LR and the MCs/NOD-R mixture. However, both M. aeruginosa PCC7806 and M. aeruginosa PCC7806mcyB− extracts had an opposite effect resulting in a substantial increase in cortisol levels. Our results suggest that MCs can modulate steroidogenesis, but the net effect of the M. aeruginosa metabolome on steroidogenesis is different from that of pure MC-LR and independent of MC production.

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Section: Discussionsupporting

confidence: 56%

Microcystins and Microcystis aeruginosa PCC7806 extracts modulate steroidogenesis differentially in the human H295R adrenal model

et al. 2020

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“…Gene expression was analyzed with quantitative real-time PCR (qPCR) which was conducted in a StepOnePlus Real-Time PCR System (Applied Biosystems, USA). Three antioxidation-related genes (superoxide dismutase ( SOD ), catalase ( CAT ), and glutathione peroxidase ( GPX )) were detected with this assay, and the housekeeping genes encoding ribosomal protein L8 ( RPL8 ) and 18S rRNA were used as the internal standards (Table 2) [27, 28]. The primers are shown in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Candidate Animal Disease Model of Elizabethkingia Spp. Infection in Humans, Based on the Systematic Pathology and Oxidative Damage Caused by E. miricola in Pelophylax nigromaculatus

Huang

Feng

Tang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Most species of the genus Elizabethkingia are pathogenic to humans and animals, most commonly causing meningitis. However, our understanding of the pathogenic mechanisms involved is poor and there have been few pathological studies of Elizabethkingia spp. in animals. To understand the host injury induced by Elizabethkingia spp., we established a model of E. miricola infection in the black-spotted frog (Pelophylax nigromaculatus). The systematic pathology in and oxidative damage in the infection model were investigated. Our results show that recently isolated E. miricola is a bacterium that mainly parasitizes the host brain and that neurogenic organs are the predominant sites of damage. Infection mainly manifested as severe brain abscesses, meningoencephalitis, necrotic spondylitis, and necrotic retinitis. The liver, spleen, kidney, gastrointestinal tract, and lung were also affected to varying degrees, with bacterial necrotic inflammation. P. nigromaculatus also suffered enormous damage to its oxidative system during E. miricola infection, which may have further aggravated its disease state. Our results provide a preliminary reference for the study and treatment of Elizabethkingia spp.-induced neurological diseases in animals.

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“…Journal Pre-proof ultrastructural changes in the liver (Zhang et al, 2013a). Results obtained on the testes of the same species showed a similar pattern of redox disbalance and revealed MC-induced reproductive toxicity (Jia et al, 2014;Jia et al, 2018;Zhang et al, 2013b, c).…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 75%

Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom

Gavrilović

Petrović

Radovanović

et al. 2021

Science of The Total Environment

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Effects of MCLR exposure on sex hormone synthesis and reproduction-related genes expression of testis in male Rana nigromaculata

Cited by 26 publications

References 55 publications

Microcystins and Microcystis aeruginosa PCC7806 extracts modulate steroidogenesis differentially in the human H295R adrenal model

Microcystins and Microcystis aeruginosa PCC7806 extracts modulate steroidogenesis differentially in the human H295R adrenal model

Candidate Animal Disease Model of Elizabethkingia Spp. Infection in Humans, Based on the Systematic Pathology and Oxidative Damage Caused by E. miricola in Pelophylax nigromaculatus

Hepatic oxidative stress and neurotoxicity in Pelophylax kl. esculentus frogs: Influence of long-term exposure to a cyanobacterial bloom

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