Effects of Matrices and Additives on Multiple Charge Formation of Proteins in MALDI–MS Analysis

Choi, Hyemin; Lee, Dabin; Kim, Yeoseon; Nguyen, Huu‐Quang; Han, Song; Kim, Jeongkwon

doi:10.1007/s13361-019-02213-7

Cited by 26 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4,24 Previous studies have reported that a 2-nitrophloroglucinol (2-NPG) matrix efficiently generates multiply charged protein ions. 9,[25][26][27] A recent study also reported that multiply charged protein ions were detected with high sensitivity in MALDI imaging analysis using a mixture of 2-NPG matrix and silica nanoparticles. 28 However, due to the nature of the 2-NPG matrix used to form crystal needles, 29 heterogeneous sample distributions are easily formed, resulting in poor reproducibility.…”

Section: Introductionmentioning

confidence: 93%

“…The 2-NPG matrix solution was prepared using two different additives (HCl or TFA) to compare their ability to generate multiply charged ions. 9 MALDI samples were prepared using either the DD or the FDD method. DD was conducted by loading 1 µL sample solution and then drying it under atmospheric pressure for ca.…”

Section: Sample Preparation For Maldi Time-of-flight (Tof) Msmentioning

confidence: 99%

“…The 2-NPG matrix was previously reported to generate a broad range of multiply charged protein ions. 9,25,31 However, it is difficult to generate a uniform sample distribution using the DD method with the 2-NPG matrix because it generates large numbers of crystal needles as it dries on the MALDI plate. 29 In the current study, we adopted a 2-NPG matrix and the FDD method to generate multiply charged protein ions and assess sample homogeneity for MALDI-MS protein analysis.…”

Section: Detection Of Multiply Charged Protein Ions Using Maldi-tof Msmentioning

confidence: 99%

“…1 However, this method often forms a heterogeneous sample distribution due to either a hot spot or coffee ring effect, resulting in poor reproducibility. [3][4][5] To improve this by attaining a homogeneous sample distribution, various approaches have been adopted, for example, using two combined matrices, 6,7 nanoparticles, 8 or acid additives 1,9 or applying other MALDI sample preparation methods such as the two-layer, 10 thin layer, 4,11,12 or freeze vacuum-drying method. 13 Homogeneous distribution of the mixture of matrix and sample solutions was also achieved by stirring the mixture thoroughly with a micropipette 14 or a glass rod.…”

Section: Introductionmentioning

confidence: 99%

“…The generation of multiply charged ions is an important factor improving mass accuracy and sensitivity in MALDI-MS analysis of macromolecules such as proteins, as it narrows the m/z range. 9 Multiply charged protein ions were obtained using various methods such as glycerol application with a CHCA matrix, 20 acetone redeposition with a 2,5-dihydroxybenzoic acid (DHB) matrix, 21 static charges on non-porous polyurethane membrane with a sinapinic acid matrix, 22 and infrared laser desorption/ionization with a caffeic acid matrix. 23 The FDD method produces a homogeneous sample distribution and generates highly multiply charged ions 3,18 because it promotes a secondary nucleation to form microcrystals, generating a homogeneous distribution of the matrix-analyte mixture.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Detection of multiply charged protein ions using matrix-assisted laser desorption/ionization mass spectrometry and a force-dried droplet method with a 2-nitrophloroglucinol matrix

Yun

Jalaludin

Jung

et al. 2022

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 93%

Section: Sample Preparation For Maldi Time-of-flight (Tof) Msmentioning

confidence: 99%

Section: Detection Of Multiply Charged Protein Ions Using Maldi-tof Msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of multiply charged protein ions using matrix-assisted laser desorption/ionization mass spectrometry and a force-dried droplet method with a 2-nitrophloroglucinol matrix

Yun

Jalaludin

Jung

et al. 2022

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

Direct sub‐atmospheric pressure ionization mass spectrometry: Evaporation/sublimation‐driven ionization is amazing, fundamentally, and practically

Trimpin,

Inutan,

Pagnotti

et al. 2024

J Mass Spectrom

View full text Add to dashboard Cite

This paper covers direct sub‐atmospheric pressure ionization mass spectrometry (MS). The discovery, applications, and mechanistic aspects of novel ionization processes for use in MS that are not based on the high‐energy input from voltage, laser, and/or high temperature but on sublimation/evaporation within a region linking a higher to lower pressure and modulated by heat and collisions, are discussed, including how this new reality has guided a series of discoveries, instrument developments, and commercialization. A research focus, inter alia, is on how best to understand, improve, and use these novel ionization processes, which convert volatile and nonvolatile compounds from solids (sublimation) or liquids (evaporation) into gas‐phase ions for analysis by MS providing reproducible, accurate, sensitive, and prompt results. Our perception on how these unprecedented versus traditional ionization processes/methods relate to each other, how they can be made to coexist on the same mass spectrometer, and an outlook on new and expanded applications (e.g., clinical, portable, fast, safe, and autonomous) is presented, and is based on ST's Opening lecture presentation at the Nordic Mass spectrometry Conference, Geilo, Norway, January 2023. Focus will be on matrix‐assisted ionization (MAI) and solvent‐assisted ionization (SAI) MS covering the period from 2010 to 2023; a potential paradigm shift in the making.

show abstract

A tutorial: Laserspray ionization and related laser‐based ionization methods for use in mass spectrometry

Trimpin

2023

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This Tutorial is to provide a summary of parameters useful for successful outcomes of laserspray ionization (LSI) and related methods that employ a laser to ablate a matrix:analyte sample to produce highly charged ions. In these methods the purpose of the laser is to transfer matrix‐analyte clusters into the gas phase. Ions are hypothesized to be produced by a thermal process where emitted matrix:analyte gas‐phase particles/clusters are charged and loss of matrix from the charged particles leads to release of the analyte ions into the gas phase. The thermal energy responsible for the charge‐separation process is relatively low and not necessarily supplied by the laser; a heated inlet tube linking atmospheric pressure with the first vacuum stage of a mass spectrometer is sufficient. The inlet becomes the “ion source”, and inter alia, pressure, temperature, and the matrix, which can be a solid, liquid, or combinations, become critical parameters. Injecting matrix:analyte into a heated inlet tube using laser ablation, a shockwave, or simply tapping, all produce the similar mass spectra. Applications are provided that showcase new opportunities in the field of mass spectrometry.

show abstract

Effects of Matrices and Additives on Multiple Charge Formation of Proteins in MALDI–MS Analysis

Cited by 26 publications

References 23 publications

Detection of multiply charged protein ions using matrix-assisted laser desorption/ionization mass spectrometry and a force-dried droplet method with a 2-nitrophloroglucinol matrix

Detection of multiply charged protein ions using matrix-assisted laser desorption/ionization mass spectrometry and a force-dried droplet method with a 2-nitrophloroglucinol matrix

Direct sub‐atmospheric pressure ionization mass spectrometry: Evaporation/sublimation‐driven ionization is amazing, fundamentally, and practically

A tutorial: Laserspray ionization and related laser‐based ionization methods for use in mass spectrometry

Contact Info

Product

Resources

About