Detection of multiply charged protein ions using matrix-assisted laser desorption/ionization mass spectrometry and a force-dried droplet method with a 2-nitrophloroglucinol matrix

Abstract

Conventional dried droplet (DD) methods show poor reproducibility in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) due to the frequent induction of a heterogeneous sample distribution. Recently, a forced dried droplet...

Matrix‐assisted laser desorption/ionization‐Fourier‐transform ion cyclotron resonance‐mass spectrometry analysis of exosomal lipids from human serum

“…21 The dried droplet method is currently the most commonly used sample preparation method; however, due to the high heterogeneity of the matrix surface, other methods, such as thin-layer and two-layer methods, have been introduced. 22,23 Fourier-transform ion cyclotron resolution (FTICR)-MS was used for the detection of ionized lipids from MALDI to compensate for the lack of chromatography and to ensure high resolution and accuracy of lipid analysis. We compared the identified lipid species from human serum and human serum exosomes and highlighted the differences in spectra across all matrices and sample preparation procedures.…”

“…MALDI sample preparation methods, such as dried droplet, two‐layer, and thin‐layer methods, were also considered as variables for comparison 21 . The dried droplet method is currently the most commonly used sample preparation method; however, due to the high heterogeneity of the matrix surface, other methods, such as thin‐layer and two‐layer methods, have been introduced 22,23 . Fourier‐transform ion cyclotron resolution (FTICR)‐MS was used for the detection of ionized lipids from MALDI to compensate for the lack of chromatography and to ensure high resolution and accuracy of lipid analysis.…”