Effects of Marrow Penetration on Bone Augmentation Within a Titanium Cap in Rabbit Calvarium

Min, Seiko; Sato, Shuichi; Murai, Masakazu; Okuno, Kenji; Fujisaki, Yoshiaki; Yamada, Yutaka; Ito, Koichi

doi:10.1902/jop.2007.070086

Cited by 32 publications

(37 citation statements)

References 22 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There was no bone marrow space or trabecular bone structure. In our previous study using a rabbit model of bone augmentation we observed that the newly generated tissue consisted of varying amounts of slender mineralized bone and large marrow spaces (4,10). These disparities might result from interspecies differences in the characteristics of the parietal bone structure.…”

Section: Discussionmentioning

confidence: 97%

“…The ability of guided bone augmentation (GBA) to produce bone has been documented in animal experiments and clinical studies (1)(2)(3)(4), and a GBA method that uses space-making barrier membranes to promote new bone growth beyond the skeletal envelope has been developed (5-7). Strictly speaking, GBA involves creating new bone by guiding bone cells to an area beyond the original outer or inner skeletal envelope.…”

Section: Introductionmentioning

confidence: 99%

“…Min et al (13) used R_mCT to observe the dynamics of bone augmentation without graft materials in rabbit calvaria. In a previous study (14), we used R_mCT to examine bone augmentation beyond the skeletal envelope in rat calvaria, A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium Go Kochi 1) , Shuichi Sato 2,3) , Hajime Ebihara 2) , Jiro Hirano 2) , Yoshinori Arai 4) and Koichi Ito Original with or without HA. The present study evaluated whether R_mCT was equivalent to histomorphometry in assessing bone augmentation beyond the skeletal envelope in rat calvaria.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

et al. 2010

Self Cite

View full text Add to dashboard Cite

Microfocus computed tomography (micro-CT; R_mCT) is a dynamic noninvasive method for measuring bone regeneration. This study evaluated whether R_mCT was equivalent to histomorphometry in assessing bone augmentation. Two plastic caps of graft material with (experiment) or without hydroxyapatite (HA; control) were placed in the exposed calvaria of rats. Images of bone augmentation within the plastic caps were then taken using R_mCT. Histological sections were cut along the same plane as that used for the micro-CT images. Bone regeneration beyond the skeletal envelope occurred at both the experimental and control sites. Bone volume also increased at both sites. In addition, consistent patterns of bone formation were observed in both R_mCT and histological images. R_mCT analysis enables highly quantitative and qualitative measurement of bone augmentation in living animals. (J Oral Sci 52, 203-211, 2010)

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…We previously developed a guided bone augmentation model in a titanium cap in rabbit calvarium (8,(32)(33)(34) and histologically observed new bone beyond the skeletal envelope. However, a further understanding of the potential role of osteoblasts in bone augmentation is required to gain mechanistic insight.…”

Section: Discussionmentioning

confidence: 99%

The in vitro osteogenetic characteristics of primary osteoblastic cells from a rabbit calvarium

et al. 2008

Self Cite

View full text Add to dashboard Cite

Previously, we showed that recombinant human bone morphogenetic protein-2 (rhBMP-2) increased bone augmentation beyond the skeletal envelope within a titanium cap in a rabbit calvarium; many cuboidal osteoblastic cells were observed histologically. These results suggested that the new osteoblastic cells might have differentiated and matured via stimulation by rhBMP-2. To date, however, no studies have reported the characteristics of osteoblastic cells derived from adult rabbit calvarium, after addition of rhBMP-2. To determine the effects of rhBMP-2 on osteoblastic cells, we observed morphological characteristics and alkaline phosphatase activity of osteoblastic cells from an adult rabbit calvarium. The expression of proteins in the BMP signaling pathway and extracellular matrix were analyzed, and mineralized nodule formation was assessed. The alkaline phosphatase activity increased significantly after rhBMP-2 stimulation. The protein levels of phosphorylated-Smad1, Runx2, osteocalcin, osteopontin, and type I collagen were augmented by rhBMP-2 stimulation using Western blotting or ELISA; rhBMP-2 also stimulated mineralized nodule formation with alizarin red staining. The results suggest that primary osteoblastic cells derived from a rabbit calvarium have osteogenetic characteristics in vitro, underscoring the potential use of these cells as a model for studying bone formation. These cells may play an important role in in vivo bone augmentation in a rabbit experimental model. (J. Oral Sci. 50, [427][428][429][430][431][432][433][434] 2008)

show abstract

“…In previous studies 13,14) , we have established that it was difficult to augment bone beyond the skeletal envelope within an occlusive space, despite the use of different biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Effects of α-DT cement with hydroxypropyl cellulose on bone augmentation within a titanium cap in the rabbit calvarium

Fukuyama¹,

Sato²,

Fukase³

et al. 2010

Dent. Mater. J.

Self Cite

View full text Add to dashboard Cite

The present study was performed to evaluate the effects of calcium phosphate cement (α-DT cement) containing α-TCP, dicalcium phosphate anhydrous, and tetracalcium phosphate mixed with hydroxypropyl cellulose (HPC) for bone augmentation within a titanium cap in the rabbit calvarium. A total of 24 male adult Japanese white rabbits were used in this study. In each rabbit, one side of the cap was filled with α-DT cement (α-DT) or α-DT cement mixed with HPC (α-DTH), and the other side of the cap was left empty (control). After 1 and 3 months, newly generated tissue and mineralized bone areas were measured histomorphometrically. Significant differences in newly generated tissue and mineralized tissue were observed between the α-DT or α-DTH group and the control at 3 months. Furthermore, α-DTH showed significantly more mineralized tissue than α-DT group. Our findings indicated that although α-DT cement was effective for bone augmentation, α-DTH was more useful than α-DT for bone maturation.

show abstract

Effects of Marrow Penetration on Bone Augmentation Within a Titanium Cap in Rabbit Calvarium

Abstract: In the rabbit calvarium using a titanium cap GBA model, bone augmentation was significantly greater with marrow penetration than without penetration.

Cited by 32 publications

References 22 publications

A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium

The in vitro osteogenetic characteristics of primary osteoblastic cells from a rabbit calvarium

Effects of α-DT cement with hydroxypropyl cellulose on bone augmentation within a titanium cap in the rabbit calvarium

Contact Info

Product

Resources

About