Effects of mannosylated glycopolymers on specific interaction to bone marrow hematopoietic and progenitor cells derived from murine species

Abstract

Poly[N-pvinylbenzyl-O-D-galactopyranosyl-(1-4)-D-glucoamide], poly[N-pvinylbenzyl-O-D-glucopyranosyl-(1-4)-D-glucoamide], and poly[N-p-vinylbenzyl-O-mannopyranosyl-(1-4)-D-gluconamide] (referred to as PVLA, PVMA, and PV-Man) are polystyrene derivatives that contain galactose, glucose, and mannose moieties, which interact with hematopoietic cells (HCs). To clarify the specific interaction between the glucopolymers and hematopoietic cells, glycopolymers labeled with fluorescent isothiocyanate (FITC) were used to follow the specific interaction, which was visualized by confocal laser microscopy. We found that PV-Man binds strongly to HCs, probably because of a specific interaction mediated by specific receptors present on the cell membrane, while some cytotoxicity when was observed when PV-Man interacted with the cell membrane. The fluorescence intensity between PV-Man and HCs was up to four-fold (0.14 +/- 0.04) that of PVMA and PVLA with hematopoietic HCs (0.033 +/- 0.01). Moreover, cellular fluorescence increased significantly with increasing incubation time and increasing polymer concentration. Using hematopoietic lineage-specific antibodies, cells were stained and analyzed by flow cytometry to confirm which HCs showed specific binding with glycopolymers, especially hematopoietic stem cells and progenitor cells (c-kit+), B-lymphocyte progenitor cells (B220+), monocyte cells (CD11b+), and erythrocytes (Ter119+).

Interaction of glycopolymers with human hematopoietic cells from cord blood and peripheral blood

“…20 In this study, we confirm the possibility of the binding activity of polymers with human hematopoietic cells derived from CB and PB. Using FITClabeled glycopolymers, we tested the dose dependent binding effects of polymers with human hematopoietic cells from CB and PBL.…”

“…In a previous report, PV Mannose showed higher binding capacity than PV Maltose and PV Lactose glycopolymers to murine hematopoietic cells. 20 Therefore, human and murine hematopoietic cells show a similar glycopolymer binding profile.…”

“…20 To assess whether glycopolymers might exert similar effects on human hematopoietic cells, cells from both organs treated with a range of glycopolymers, PV Mannose and PV Maltose. After binding for 1 h, cells were stained with propidium iodide to determine the percentage of apoptotic cells by flow cytometry.…”

Role of Endothelial N-Glycan Mannose Residues in Monocyte Recruitment During Atherogenesis

“…Mac1 has been shown to bind type-I fimbriated E. coli in a mannose dependent manner 53 and CD11b+ monocytes shows high binding affinity to mannosylated glycopolymers 54 . Soluble CD11b demonstrates elevated binding to ICAM-1 produced in the presence of alpha-mannosidase 1 inhibitors 55 suggesting that increased mannose on ICAM-1 promotes binding.…”

Cellulose Chemistry Meets Click Chemistry: Syntheses and Properties of Cellulose-Based Glycoclusters with High Structural Homogeneity

“…The low water solubility of Cel-O-Lac probably arises from its hydrophobic -face of non-reducing -galactoside units. The observed negative effects arising from the hydrophobic spacers and the -face on their water solubility are much more drastic in comparison with those observed for glycoclusters, based on flexible artificial polymers such as polystyrene [34][35][36][37][38][39][40] and polyacrylamide [41][42][43][44][45][46]. For example, polystyrenes having -lactoside appendages still have excellent water solubility.…”