Interaction of glycopolymers with human hematopoietic cells from cord blood and peripheral blood

Abstract

Polystyrene derivatives, poly[N-pvinylbenzyl-O-D-glucopyranosyl-(1-4)-D-glucoamide] (PV Maltose) and poly[N-p-vinylbenzyl-O-mannopyranosyl-(1-4)-D-glucoamide] (PV Mannose), which contain glucose and mannose moieties, respectively, have the specific binding ability with murine hematopoietic cells. In this study, we confirm the ability of these glycopolymers to interact specifically with human hematopoietic stem cells (HSCs) and mature cells derived from human cord blood (CB) and peripheral blood (PB). Using fluorescence isothiocyanate (FITC)-labeled glycopolymers, we observed that 98% to 93% of hematopoietic cells interacted very strongly with PV Mannose, and 63% of CB and 29% PB interacted with PV Maltose. Both glycopolymers bound better to cells from CB than from PB. Cytotoxic studies revealed that a 0.1 mM dose of PV Mannose induced apoptosis in 20% CB cells, in contrast to 3-5% PB cells. Furthermore, we demonstrated that all of CD34(+) HSCs of both origins bound specifically to PV Mannose, whereas 33-47% bound to PV Maltose. In addition, the majority of B cells (CD19(+)), T cells (CD3(+)), monocytes (CD14(+)), and erythrocytes (CD235a(+)) bound to PV Mannose, but a lower percentage interacted with PV Maltose. In vivo study, bone marrow, spleen, and liver tissues in NOD-SCID mice injected with PV Mannose conjugated CB, were detected PV Mannose positive hematopoietic cells. These data suggest that the use of PV Mannose and PV Maltose might be used for gene and drug delivery for hematopoietic cells and thus, may be useful in therapeutic settings.

Applications of mannose-binding lectins and mannan glycoconjugates in nanomedicine

“…Poly[N-p-vinylbenzyl-O- d -glucopyranosyl-(1–4)- d -glucoamide] (PV-Maltose) and PV-Mannose, which contain glucose and mannose moieties, respectively, have specific binding ability with murine haematopoietic cells. PV-Mannose and PV-Maltose have also been proposed for gene and drug transfer to haematopoietic cells in clinical situations [ 161 ].…”

Synthesis, Photophysical, Electrochemical Studies, and Antioxidant Properties of Fluorescein‐Linked Glycodendrimers

“…[2] Fluorescein hybrids with sugar molecules have potential applicationsi nd rug discoverya nd gene delivery systems. [3] Fluorescein-labeled glucose derivatives are used in bioimaging drugs for neurodegeneratived iseases, [4] cancer therapy, [5] as biosensors, [6] and as protein-based indicators [7] as well as reliable platforms for fluorescent probesf or biomolecules, such as protein and DNA. [8] Glycodendrimers function as apotentialplatform for many biological applicationsa saresult of their highly branched threedimensional architectures and the multivalency property of the surfaceg roups.…”