Effects of major nutrients, growth regulators and inoculum size on enhanced growth and camptothecin production in adventitious root cultures of Ophiorrhiza mungos L.

Deepthi, S.; Satheeshkumar, K.

doi:10.1016/j.bej.2016.10.016

Cited by 48 publications

(11 citation statements)

References 58 publications

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“…In many recent studies it had been shown that composition of the culture medium that includes nutrients, carbon sources and osmotic stabilizers can be manipulated to create in vitro drought conditions, with effect on metabolic processes that may lead to biomass and SMs accumulation [101,177,178]. For instance, media nutrients manipulation influenced biomass and CPT production in Nothapodytes nimmoniana [179,180] and Ophiorrhiza mungos [181]. The strength of the culture medium nutrients influenced phenolic compounds profile of Bellis perennis calli through the induction of antioxidant system due to increased stress condition [182].…”

Section: Droughtmentioning

confidence: 99%

Stress and defense responses in plant secondary metabolites production

2019

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In the growth condition(s) of plants, numerous secondary metabolites (SMs) are produced by them to serve variety of cellular functions essential for physiological processes, and recent increasing evidences have implicated stress and defense response signaling in their production. The type and concentration(s) of secondary molecule(s) produced by a plant are determined by the species, genotype, physiology, developmental stage and environmental factors during growth. This suggests the physiological adaptive responses employed by various plant taxonomic groups in coping with the stress and defensive stimuli. The past recent decades had witnessed renewed interest to study abiotic factors that influence secondary metabolism during in vitro and in vivo growth of plants. Application of molecular biology tools and techniques are facilitating understanding the signaling processes and pathways involved in the SMs production at subcellular, cellular, organ and whole plant systems during in vivo and in vitro growth, with application in metabolic engineering of biosynthetic pathways intermediates.

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Section: Droughtmentioning

confidence: 99%

Stress and defense responses in plant secondary metabolites production

2019

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“…g −1 DW SH Monforte-González et al ( 2019 ) Camptothecin Ophiorrhiza mungos 0.86 mg⋅g −1 DW 1.12 mg . g −1 DW AR S Deepthi and Satheeshkumar ( 2017a ); Deepthi and Satheeshkumar ( 2017b ) Capsaicin & dihydrocapsaicin Capsicum chinense 2.87 / 1.03 mg . g −1 DW S Kabita et al ( 2020 ) Indole alkaloids Isatis tinctoria 3.15 mg .…”

Section: Production Via Plant In Vitro Culturesmentioning

confidence: 99%

“…Through cell line selection, optimization of the nutrient medium, and elicitation with jasmonic acid, the authors achieved a camptothecin production of 1.12 mg⋅g −1 DW (dry weight) compared to 0.06 mg⋅g −1 DW in the original cell line. The accumulation of camptothecin in adventitious roots of Ophiorrhiza mungos has been studied by Deepthi and Satheeshkumar ( 2017a ), who showed that camptothecin production was influenced by the nutrient medium (concentration of major nutrients, plant growth regulators) and by inoculum size. Under optimized conditions the in vitro cultures produced 0.86 mg⋅g −1 DW of the alkaloid, which was well above the concentration of 0.15 mg⋅g −1 DW found in the roots of the donor plant used for culture establishment.…”

Section: Alkaloidsmentioning

confidence: 99%

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Wawrosch

Zotchev

2021

Appl Microbiol Biotechnol

106

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Medicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessitates the processing of large amounts of field-cultivated raw material. The biotechnological manufacturing of such compounds offers a number of advantages like predictable, stable, and year-round sustainable production, scalability, and easier extraction and purification. Plant cell and tissue culture represents one possible alternative to the extraction of phytochemicals from plant material. Although a broad commercialization of such processes has not yet occurred, ongoing research indicates that plant in vitro systems such as cell suspension cultures, organ cultures, and transgenic hairy roots hold a promising potential as sources for bioactive compounds. Progress in the areas of biosynthetic pathway elucidation and genetic manipulation has expanded the possibilities to utilize plant metabolic engineering and heterologous production in microorganisms. This review aims to summarize recent advances in the in vitro production of high-value plant secondary metabolites of medicinal importance.Key points• Bioactive plant secondary metabolites are important for current and future use in medicine• In vitro production is a sustainable alternative to extraction from plants or costly chemical synthesis• Current research addresses plant cell and tissue culture, metabolic engineering, and heterologous production Graphical abstract

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“…Recently, CPT producing Ophiorrhiza plants have become viable alternative sources for CPT production by tissue culture (82,97). O. pumila (67), O. mungos (98)(99)(100), O. alata (77), O. liukiuensis, O. kuroiiwai (12), O. rugosa (73), O. rugosa var. decumbens (79), O. rugosa var.…”

Section: Bioproduction Studiesmentioning

confidence: 99%

Ophiorrhiza, a promising herbaceous source of the anticancer compound camptothecin

et al. 2020

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Camptothecin is an important source for the synthesis of some of the major anti-cancer agents such as irinotecan and topotecan. Traditional source of camptothecin are prominently woody plants such as Camptotheca acuminata Decne. and Nothopodytes nimmoniana (Graham) Mabb., and the increasing demand for camptothecin leads to the level of threatening their existence. Ophiorrhiza species composed of herbaceous plants with quick growth characteristics which are reported as alternative source of camptothecin. The present review focus on taxonomical status, traditional uses, biological activities and phytochemical constituents with a special attention in bioproduction of camptothecin from Ophiorrhiza species and its future prospects.

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Effects of major nutrients, growth regulators and inoculum size on enhanced growth and camptothecin production in adventitious root cultures of Ophiorrhiza mungos L.

Cited by 48 publications

References 58 publications

Stress and defense responses in plant secondary metabolites production

Stress and defense responses in plant secondary metabolites production

Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Ophiorrhiza, a promising herbaceous source of the anticancer compound camptothecin

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