Effects of lysine deficiency or excess on growth and the expression of lipid metabolism genes in slow-growing broilers

Tian, Di; Guo, Rongrong; Li, Y.M.; Chen, P.P.; Zi, Barbashova; Wang, J.J.; Liu, R.F.; Min, Yuna; Wang, Z.P.; Niu, Zhiwei; Liu, F.Z.

doi:10.3382/ps/pez041

Cited by 23 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is observed from Table (5) that the effect of fortifying the diets of low-protein broiler broilers at rates (5, 10, 15, 20)% with some essential AA (lysine, methionine, and threonine), respectively, is less than the recommendations of the Rose Breeding Guide 308 on the average relative weights of the internal viscera Edible and belly obesity for male broilers, the control treatment (T1) was significantly superior in the characteristic of the relative weight of the liver at a significant level (P<0.05) over the rest of the treatments, while there was no significant superiority between the (T1) and (T5). As for the relative weight of the heart, it was noted that the (T5) was significantly superior (P<0.05) over the rest of the experimental treatments except for the (T4), as there were no significant differences between the two treatments, while no significant differences were observed between the two treatments.…”

Section: Resultsmentioning

confidence: 82%

“…The primary feed materials were prepared from well-known local laboratories, and the feed was served crushed. After reducing protein in treatments (5,4,3,2) at rates (5,20,15,10) respectively, the diet was replaced with essential AA (methionine, lysine and threonine) b, the first treatment T1, the control treatment, the diet was standard and according to ROSS308 guide , the second treatment T2, in which the protein was reduced by 5% in (initiator, growth and final) and replaced (lysine, methionine and threonine) to be equivalent to what is high in the control diet, the third treatment T3 in which the protein was reduced by 10% in (initiator, growth and final) and replaced (Lysine, methionine and threonine) to be equivalent to what is high in the control diet, the fourth treatment T4 reduced the protein by 15% in (initiator, growth and final) and replaced (lysine, methionine and threonine) to be equivalent to what is high in the control diet, the fifth treatment T5 protein was reduced It has 20% in (initiator, growth and end) and replaced (lysine, methionine and threonine) to be equivalent to what is high in the control diet.SAS was Used in data analysis using Duncan ( 1955 ).…”

Section: Methodsmentioning

confidence: 99%

“…Both energy and protein constitute 85% of the total cost of feeding in these projects [3]. AA are the only structural, functional, or ctuculturali, that regulate metabolic pathways in the body [4] , stimulate protein synthesis and fat metabolism, and play an important role in increasing broiler growth [5] , in addition, to their antioxidant role, especially methionine. And lysine, threonine, and arginine improve the gut health of birds through their effect on immune globulin [6] , as these acids enter the formation of mucin, which has a role in promoting digestion and absorption processes by increasing goblet cells [7] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Reinforcement of Low-Protein Diets with some Essential Amino Acids and Their Effect on the Characteristics of a Carcass and some Blood Characteristics of Broilers

Abdullah

Albaddy

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The experiment was conducted in the poultry field of the Department of Animal Production - College of Agriculture - Tikrit University for the period from 18-25/10/2021, as the purpose of it was to strengthen low-protein diets with some essential amino acids (AA) and their effect on productive performance and some physiological characteristics of broilers. In this experiment, (300) ROSS 308 birds were bred with an average initial weight of 39.7 g. The birds were divided into five treatments, each treatment consisted of three replicates and was distributed in cages (20 birds per cage), the treatments were randomly distributed, (T1) the first treatment was treated The comparison contains protein according to the ROSS guide 308, and (T2) the second treatment in which the protein was reduced by 5% and fortified with essential AA (lysine, methionine, and threonine), and (T3) the third treatment in which the protein was reduced by 10% and was fortified with AA (Lysine, methionine, and threonine), and (T4) the fourth treatment, in which the protein was reduced by 15% and fortified with AA (Lysine, methionine, and threonine), and (T5) the fifth treatment, which reduced the protein by 20% less than the recommendations of the NRC ( 1994) and fortified with AA (lysine, methionine, and threonine). The results of this experiment showed that there were no significant differences between the experimental treatments in the clearance ratio for both males and females, while there were significant differences at a significant level (P<0.05) in both the first treatment and the fifth treatment in the characteristic of the relative weight of the liver for males higher than The rest of experimentalist treatments, whereas the 4th and 5th treatments were higher levels than the rest of experimentalist treatments in the characteristic of the relative weight of the heart of males, while there were no significant differences in each of (gizzard, spleen and belly fat) for male broilers, while the fourth treatment (T4) outperformed the third treatment. In the characteristic of the relative weight of the gizzard in female broilers, the significance of the fourth treatment and the rest of the experiment treated not differ in the same characteristic, while there were no significant differences at a significant level (P<0.05) in (liver, heart, spleen, and belly fat) in the Female broilers. As for the biochemical characteristics of male and female broilers, additionality treatments were superior in some of them, and there were no statistically significant differences in some others

show abstract

Section: Resultsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reinforcement of Low-Protein Diets with some Essential Amino Acids and Their Effect on the Characteristics of a Carcass and some Blood Characteristics of Broilers

Abdullah

Albaddy

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…PPARα was a ligand-induced transcription factor that stimulates the target genes involved in peroxisomal and mitochondrial β-oxidation ( ACO , ACADL , CPT1 ), lipogenesis ( ME ), fatty acid binding, and transport ( ACBP , FABP3 , FATP1 ) ( Finck et al., 2002 , 2005 ; Mandard et al., 2004 ; Burri et al., 2010 ). Research on broilers mentions the activation of PPARα induced the upregulation of fatty acid transport and β-oxidation ( Tian et al., 2019 ) . By overexpressing PPARα in mice, Finck et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of different rearing systems on intramuscular fat content, fatty acid composition, and lipid metabolism–related genes expression in breast and thigh muscles of Nonghua ducks

Guo

Deng

et al. 2020

Poultry Science

View full text Add to dashboard Cite

Rearing system is a critical nongenetic factor influencing meat quality of ducks. In this study, a total of 360 birds were randomly allocated into floor rearing system ( FRS ) and net rearing system ( NRS ) to compare their effects on intramuscular fat ( IMF ) deposition, fatty acid composition, and related gene expression in muscles of Nonghua ducks. Sawdust bedding and stainless mesh bed were equipped in FRS and NRS, respectively. At the eighth week ( 8w ) and 13th week ( 13w ), the breast and thigh muscles of ducks were collected to determine the profiles of lipids composition and the expressions of lipid metabolism–related genes. The IMF content was higher in 13w-FRS than 8w-FRS and 8w-NRS in breast muscle, whereas it was higher in 13w-NRS than other groups in thigh muscle ( P < 0.05). C16:1, C20:5(n-3) of muscles were higher in 8w-NRS than 8w-FRS, whereas C18:1(n-9)c, C18:2(n-6)c, Ʃ monounsaturated fatty acid ( MUFA ), and ƩMUFA/Ʃsaturated fatty acid ( SFA ) ratio of muscles were higher in 13w-NRS than 8w-FRS and 8w-NRS ( P < 0.05). C22:6(n-3), C20:4(n-6) of breast muscle and C20:3(n-6) of thigh muscle were higher in 13w-NRS than 13w-FRS ( P < 0.05). Fatty acids variation was studied by principal component analysis, exhibiting extensive positive loadings on principal components. SREBP1 , ACADL , and FABP3 were downregulated in breast muscle, whereas PPARα and ELOVL5 were upregulated in thigh muscle of NRS ducks at 13w. Principal components were extensively correlated with lipids composition parameters, and principal components of breast muscle 1 and principal components of thigh muscle 1 were correlated with SREBP1 and PPARα , respectively ( P < 0.05). In conclusion, with increasing age, FRS enhanced IMF deposition in breast muscle, and the same promotion in thigh muscle was because of NRS. The variation of fatty acids in muscles was uniform, and the change of single fatty acid was unable to distinguish NRS and FRS. However, as NRS downregulated SREBP1 , ACADL and FABP3 in breast muscle and upregulated PPARα and ELOVL5 in thigh muscle, NRS could improve nutrient value and meat quality by increasing ƩMUFA, ƩMUFA/ƩSFA ratio, and important PUFA levels. Therefore, NRS was more recommended than FRS for Nonghua ducks during week 8 to 13 posthatching.

show abstract

“…Research shows that regulatory genes that control sterol regulatory element-binding protein 1 (SREBF1), fatty acid transport protein 4 (FATP4), fatty acid synthase (FAS), and acetyl-coenzyme A carboxylase (ACC) are all involved in fat metabolism [ 7 ]. Sterol element-binding proteins (SREBPs) can influence lipid homeostasis by regulating their target genes, essential for cholesterol and fatty acid metabolism [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of bile salt diet supplementation on genes related to fat metabolism in yellow-feathered broilers

Zhang

Ding

et al. 2022

Vet World

View full text Add to dashboard Cite

Background and Aim: As a new feed additive, bile acid (BA) can promote the absorption and transport of lipids and fat-soluble vitamins. In recent years, BAs have been widely used in animal feed to promote fat absorption. Therefore, this study aimed to investigate the effect of bile salt supplementation in the diet of yellow-feathered broilers on messenger RNA (mRNA) expression of sterol regulatory element-binding protein 1 (SREBF1), fatty acid synthase (FAS), acetyl-coenzyme A carboxylase (ACC), and fatty acid transport protein 4 (FATP4). Materials and Methods: Four hundred and twenty commercial male chicks were randomly divided into seven groups (with four replicates per group and 15 chickens per replicate). They were fed diets supplemented with bile salts at 0, 1.5, 2.5, 3.5, 4.5, 5.5 mg/kg, and 2 mg/kg tylosin for 30 days. Changes in SREBF1, fatty acid transporter 4, FAS, and acetyl- CoA carboxylase genes in intestinal mucosa and liver of yellow-feathered broilers were determined using a quantitative fluorescence polymerase chain reaction. Results: mRNA expression of SREBF1, FAS, ACC, and FATP4 in the small intestine decreased in chicks fed diets supplemented with 3.5 and 4.5 mg/kg bile salts (p<0.05) compared with the control group on 7 days and 14 d. The mRNA expressions of SREBF1, FAS, ACC, and FATP4 in liver tissue decreased in chicks fed diets supplemented with 4.5 and 5.5 mg/kg bile salts (p<0.05) compared to the control group on 7 days. The mRNA expression of SREBF1, FAS, ACC, and FATP4 in the liver at 14 days and the small intestine on 21 days also decreased in chicks fed diets supplemented with 4.5 mg/kg bile salts (p<0.05) compared to the control group. When contrasted with the control group on day 21, the mRNA expression of SRWBF1, FAS, ACC, and FATP4 detected in the liver was lower in chicks fed diets supplemented with bile salts (p<0.05). Conclusion: The dietary supplementation of bile salts at 4.5 mg/kg effectively regulates the expression of fat metabolism genes, such as SREBF1, FAS, ACC, and FATP4 mRNA. At this concentration, bile salts promote fat catabolism, inhibit fat synthesis, and play an essential role in improving the fat deposition of broilers.

show abstract

Effects of lysine deficiency or excess on growth and the expression of lipid metabolism genes in slow-growing broilers

Cited by 23 publications

References 39 publications

Reinforcement of Low-Protein Diets with some Essential Amino Acids and Their Effect on the Characteristics of a Carcass and some Blood Characteristics of Broilers

Reinforcement of Low-Protein Diets with some Essential Amino Acids and Their Effect on the Characteristics of a Carcass and some Blood Characteristics of Broilers

Effects of different rearing systems on intramuscular fat content, fatty acid composition, and lipid metabolism–related genes expression in breast and thigh muscles of Nonghua ducks

The effect of bile salt diet supplementation on genes related to fat metabolism in yellow-feathered broilers

Contact Info

Product

Resources

About