Effects of &lt;i&gt;Asterias amurensis&lt;/i&gt;-derived Sphingoid Bases on the &lt;i&gt;de novo&lt;/i&gt; Ceramide Synthesis in Cultured Normal Human Epidermal Keratinocytes

Mikami, Daisuke; Sakaï, Shota; Sasaki, Shigefumi; Igarashi, Yasuyuki

doi:10.5650/jos.ess16051

Cited by 9 publications

(12 citation statements)

References 39 publications

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“…Crude sphingoid bases from A. amurensis-derived GlcCers were prepared as previously reported 8,27 . Asterias amurensis ethanol extract was separated on silica gel by gradient elution consisting of chloroform:methanol 10:0 9:1 8:2 v:v .…”

Section: Preparation and Isolation Of Sphingoid Basesmentioning

confidence: 99%

“…LC-MS/MS analysis was carried out with a TripleTOF 5600 system AB SCIEX, Foster City, CA, USA in electrospray ionization ESI -positive mode 30 as previously described 8 . Sphingolipids extracted from keratinocytes were dissolved in 100 µL of methanol:acetonitrile 1:1, v:v , and 10 µL of sample solution was injected onto an InertSustain NH2 hydrophilic interaction chromatography column particle size, 5 µm; diameter, 2.1 mm; length, 150 mm; GL Science .…”

Section: Sphingolipid Quanti Cation By Lc-ms/msmentioning

confidence: 99%

“…trienine d18:3 , and 9-methyl sphingatrienine 9Me-d18:3 23 . The sphingoid bases d18:3, 9Me-d18:3, d22:1, d22:2, and t22:1 are produced by the starfish Asterias amurensis 8,24,25 . Treatment of cultured human keratinocytes with sphingoid bases can induce differentiation and promote sphingolipid biosynthesis 7,8,26 .…”

mentioning

confidence: 99%

“…The sphingoid bases d18:3, 9Me-d18:3, d22:1, d22:2, and t22:1 are produced by the starfish Asterias amurensis 8,24,25 . Treatment of cultured human keratinocytes with sphingoid bases can induce differentiation and promote sphingolipid biosynthesis 7,8,26 . Previously, we reported that a mixture of sphingoid bases derived from A. amurensis induces the accumulation of ceramides, GlcCers, and SMs and up-regulates the mRNA expression levels of sphingolipid biosynthesis-related genes in undifferentiated keratinocytes 8 .…”

mentioning

confidence: 99%

“…Treatment of cultured human keratinocytes with sphingoid bases can induce differentiation and promote sphingolipid biosynthesis 7,8,26 . Previously, we reported that a mixture of sphingoid bases derived from A. amurensis induces the accumulation of ceramides, GlcCers, and SMs and up-regulates the mRNA expression levels of sphingolipid biosynthesis-related genes in undifferentiated keratinocytes 8 . In the present study, we isolated individual A. amurensis-derived sphingoid bases using a novel method involving Nacetyl derivatization and high-performance liquid chromatography HPLC with an octadecyl silyl ODS column.…”

mentioning

confidence: 99%

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Isolation of Sphingoid Bases from Starfish <i>Asterias amurensis</i> Glucosylceramides and Their Effects on Sphingolipid Production in Cultured Keratinocytes

et al. 2019

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Introduction Sphingolipids are not only components of cellular membranes but also function as signaling molecules 1 3. Many studies have shown that treatment of keratinocytes with sphingolipids accelerates differentiation 4 6 and de novo production of ceramides 7, 8. Ceramides are major components of stratum corneum lipids that perform important barrier functions 9. Patients with atopic dermatitis AD have fewer skin ceramides and altered ceramide composition compared to those without AD 10, 11. Ceramide synthase 3 CerS3 and elongase of very long chain fatty acids ELOVL 1 and 4 are essential enzymes that produce ultralong-chain fatty acid ULCFA-bound ceramides, which are specifically expressed in skin tissues 12 14. Mice lacking these genes die within several hours after birth because of rapid water loss from the skin 12 14. Thus, the levels of skin ceramides and their fatty acid chain lengths are important factors in skin barrier functions.

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Section: Preparation and Isolation Of Sphingoid Basesmentioning

confidence: 99%

Section: Sphingolipid Quanti Cation By Lc-ms/msmentioning

confidence: 99%