Effects of low-level laser therapy on human osteoblastic cells grown on titanium

Abstract

The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.

Histomorphometric Assessment of the Influence of Low-Level Laser Therapy on Peri-Implant Tissue Healing in the Rabbit Mandible

“…[4][5][6][7] Experimental studies on the effect of LLLT on peri-implant bone healing have shown improved osseointegration and a shorter implant rehabilitation period. [8][9][10][11][12][13][14][15][16][17] The rabbit has been the preferred species for research on the process of osseointegration after LLLT. An advantage of rabbits over other animal models is that they are less aggressive, relatively inexpensive to purchase, and easy to house and maintain.…”

Influence of low-level laser on dental implant sites: A literature review

“…In vitro studies investigating the effect of LLLT on human osteoblasts cultured on titanium evinced increased cell adhesion, 14 proliferation, 14,15 diferentiation, 14,16 as well as increased osteocalcin and transforming growth factor β1 (TGF-β1) synthe- Diferent study methods were employed to assess the eicacy of LLLT on implant anchorage sites with a view to elucidating the efects of laser on osseointegration. Biomechanical removal torque tests were used to assess the shear strength at the bone-implant interface and provide a correlation between the force required to remove an implant and the degree of contact with the bone.…”

Effect of Diode Laser on Healing of Tooth Extraction Socket: An Experimental Study in Rabbits

“…Some studies focused on osteoblastic differentiation using LLLT. The results of three studies showed that LLLT was effective in the stimulation of osteoblasts differentiation [31][32][33]. On the other hand Bouvet-Gerbettaz et al [34] and Coombe et al [35] showed no beneficial effect of laser on the proliferation and differentiation of osteoblasts.…”