Effects of Low-Level Laser Irradiation on the Pathogenicity of<i>Candida albicans</i>:<i>In Vitro and in Vivo</i>Study

Seyedmousavi, Seyedmojtaba; Hashemi, Seyed Jamal; Rezaie, Sasan; Fateh, Mohsen; Djavid, Gholamreza Esmaeeli; Zibafar, Ensieh; Morsali, Farhad; Zand, Nasrin; Alinaghizadeh, Mohammadreza; Ataie‐Fashtami, Leila

doi:10.1089/pho.2012.3387

Cited by 22 publications

(29 citation statements)

References 35 publications

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“…The UVR effect on fungal metabolism was related to DNA degradation in conidia and mycelium of Aspergillus nidulans (Braga et al 2015). In addition, Seyedmousavi et al (2014) reported that UVR affected proteolytic activity, cell growth, and carbohydrate synthesis in Candida albicans. Besides, it had also been reported that UV-B inhibited various fungal processes such as spore germination and hyphae elongation (Suthaparan et al 2016) and affects negatively several fungi such as Botryris cinerea (Janisiewicz et al 2016).…”

Section: Oil Formulationsmentioning

confidence: 99%

Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings

Herrera

Valbuena

Pavone-Maniscalco

2020

Egypt J Biol Pest Control

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Environmental conditions affect biocontrol agents in a field, being appropriate formulations an alternative to overcome this problem. Formulations based on Trichoderma asperellum TV190 were prepared by emulsified mineral or vegetable oils, which protected spores from ultraviolet radiation, showing greater viability of 37-43% (mineral) and 56-63% (vegetable) than the control (8-12%). These formulations improved an antagonism of T. asperellum on Rhizoctonia solani under greenhouse conditions, reducing infected corn seedlings by 72% (mineral) and 59% (vegetable). Necrotic spot size was reduced by 90.04% (mineral) and 87.29% (vegetable). A granular formulation, prepared with degreased corn germ and T. asperellum spores, protected the corn seedlings from R. solani under greenhouse conditions, with 73% reduction of infected plants and 93% reduction of necrotic spot size. Both granular and liquid formulations were able to improve T. asperellum antagonism, suggesting that these formulations could be included in agricultural pest control strategies.

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Section: Oil Formulationsmentioning

confidence: 99%

Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings

Herrera

Valbuena

Pavone-Maniscalco

2020

Egypt J Biol Pest Control

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“…Management of infections caused by bacteria and fungi is a viable challenge in various medical fields, including dentistry. It cannot be emphasized enough that we need to focus on the search for alternative methods to manage rapidly developing drug resistance and recurrent candidiasis [ 10 ]. The development of laser medicine has provided a number of new therapy modalities capable of damaging pathogenic organisms.…”

Section: Introductionmentioning

confidence: 99%

Effects of Nd:YAG laser irradiation on the growth of Candida albicans and Streptococcus mutans: in vitro study

et al. 2018

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The purpose of this study was to evaluate the effects of Nd:YAG laser with flat-top handpiece on the in vitro growth of Candida albicans and Streptococcus mutans. The incidence of C. albicans (opportunistic commensal) and S. mutans (facultatively anaerobic) infections is increasing, despite available treatments. Cultures of Streptococcus mutans and Candida albicans were irradiated using Nd:YAG laser (LightWalker, Fotona) with flat-top handpiece (Genova, LightWalker, Fotona) at the following parameters: group G1: 0.25 W, 10 Hz, 15 s, 3 J and group G2: 1 W, 10 Hz, 60s, 59 J. The results were evaluated directly and 24 h after irradiation using a quantitative culture method (estimation of colony-forming units in 1 ml of suspension, cfu/ml), and microscopic analysis with Janus green stain and compared with control group in which laser was not applied. C. albicans was reduced by 20 up to 54% for G1, and for G2 by 10 up to 60% directly after the application. The cfu/ml values for S. mutans decreased by 13% (p = 0.1771) for G1 and 89% (p < 0.0001) for G2. In both test groups 24 h after the application, the number of colony-forming units decreased by 15-46% for G1 and by 15-64% for G2. The arrested cell division, increasing the surface area and increasing the number of metabolically inactive cells, were observed in morphometric analysis. Macroscopic and microscopic analyses revealed a reduction in cell number and a significant decrease of cell metabolism after laser application for both C. albicans and S. mutans.

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“…We believe that this fact occurred because this chemical compound acted as a toxic agent to the bacterium pseudomonas aeruginosa interfering in its natural physiological reactions. If this hypothesis is correct, we observed that the low-level laser acted as a chemical enhancement of the reaction between the methylene blue and the bacterium, increasing its elimination [31]. This study demonstrated that photodynamic therapy is able to reduce the proliferation of pseudomonas aeruginosa.…”

Section: Discussionmentioning

confidence: 66%

Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

Mm¹

2018

VIJ

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Pressure ulcers are injuries that can cause bacterial proliferation, and when infected are associated with increased morbidity and mortality. Thus, photodynamic therapy becomes an adjunct to the treatment with medications because it has the bactericidal capacity. In this way, this study aims to analyze in vitro the bactericidal effect of methylene blue by associating it with the low-level laser in the wavelength of 830nm to determine which therapeutic doses present better efficacy in the treatment of bacteria pseudomonas aeruginosa. The microbiological material was collected by an aseptic swab and the antimicrobial activity was verified by disc diffusion method using sterile filter paper. Methylene blue was used at concentrations of 0,001% and 0,005% and the low-level laser of 830nm at the intensities of 4J/cm², 8J/cm², 10J/cm² and 14J/cm², being tested in three plates for each dose, as well as the low-level laser and the methylene blue, were tested separately. The results were statistically analyzed by SPSS through the "t-test for dependent samples". It was verified that there was reduction of the bacterial activity with the application of the low-level laser in doses of 10J/cm² (p=0,00) and 14J/cm² (p=0,03) added to them ethylene blue in 0.005% concentration, however, there was a greater reduction of bacterial proliferation in the application of 10J/cm². Thus, it was concluded that the photodynamic therapy applied in the concentration of 0.005% of the methylene blue combined with the low-level laser of 830nm of 10J/cm² and 14J/cm² of intensity, present a reduction in the amount of bacterial proliferation.Once the bacteria multiply in the pressure ulcer, new clinical signs and symptoms are observed, worsening the Research ArticleVolume 2 Issue 1

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Effects of Low-Level Laser Irradiation on the Pathogenicity ofCandida albicans:In Vitro and in VivoStudy

Cited by 22 publications

References 35 publications

Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings

Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings

Effects of Nd:YAG laser irradiation on the growth of Candida albicans and Streptococcus mutans: in vitro study

Physiotherapeutic Intervention with Biofotododynamic Therapy for the Reduction of Pseudomonas Aeruginosa

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