Effects of low-density lipoproteins on blood coagulation and fibrinolytic activity

Азизова, О. А.; Roitman, Eytan; Dement'eva, I I; Nikitina, N. A.; Gagaeva, E. V.; Lopukhin, Yu. M.

doi:10.1007/bf02434870

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…It has been suggested that fibrin may be directly affected by highly oxidized LDL altering the ensuing clot network by enhancing hypercoagulation and fibrinolytic resistance in comparison to nonoxidized LDL. 48 These results suggest a potential, novel role for LDL-C in thrombotic disease, which deserves further investigation. Intrafibrillar fibrin structure has recently been demonstrated to be modifiable and to significantly influence clot mechanical properties and resistance to lysis.…”

Section: Discussionmentioning

confidence: 86%

Interpretation and Validation of Maximum Absorbance Data Obtained from Turbidimetry Analysis of Plasma Clots

et al. 2019

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Turbidimetry is used to characterize fibrin clot properties. In purified systems, maximum absorbance (MA) directly relates to fibrin fiber cross-sectional area. However, in plasma samples there are discrepancies in the relationships between MA and fibrinogen concentration, fiber diameter, other clot properties, and cardiovascular disease outcomes, which complicate data interpretation. This study aims to advance understanding of MA of plasma clots through testing how well it relates to fundamental dependence on fibrinogen concentration and fiber diameter as predicted by light scattering theory, other clot properties and lifestyle, and biochemical variables. Plasma samples from 30 apparently healthy individuals with a fibrinogen concentration from 2.4 to 6.4 g/L were included. We performed turbidimetry, permeability, scanning electron microscopy, and rheometry on in vitro formed plasma clots. MA correlated more strongly with fibrinogen concentration (r = 0.65; p < 0.001) than with fiber diameter (r = 0.47; p = 0.01), which combined explained only 46% of the MA variance. Of additional variables measured, only low-density lipoprotein cholesterol correlated with MA (r = 0.46; p = 0.01) and clot lysis (r = 0.62; p < 0.0001) but not with fiber diameter or fibrinogen concentration. MA correlated with clot lysis time (r = 0.59; p = 0.001), storage modulus (r = 0.61; p = 0.001), and loss modulus (r = 0.59; p = 0.001), and negatively with clot permeability (r = –0.60; p = 0.001) also after adjustment for fibrinogen concentration and fiber diameter. Increased MA is indicative of a prothrombotic clot phenotype irrespective of fibrinogen concentration. MA is more indicative of overall clot density than of fiber diameter. Other plasma components can alter internal fiber density without altering fiber diameter and should be considered when interpreting MA of plasma samples.

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Section: Discussionmentioning

confidence: 86%

Interpretation and Validation of Maximum Absorbance Data Obtained from Turbidimetry Analysis of Plasma Clots

et al. 2019

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“…These results determined in the study show similarity to the results of other investigators (Hill and Matrone, 1961; Simpson et al., 1963; Simpson and Harms, 1964; Carr and Lei, 1989; Boyne and Arthur, 1990). Azizova et al. (2000) reported that, in in vitro experiments, Cu‐oxidized LDL activate factors of the prothrombin complex in the whole blood and inhibit fibrin generation in both blood and plasma.…”

Section: Discussionmentioning

confidence: 99%

Effect of Copper Deficiency on Blood Lipid Profile and Haematological Parameters in Broilers

Kaya

Altıner

Özpınar

2006

Journal of Veterinary Medicine Series A

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The aim of the study was to observe the changes in haematological parameters and blood lipid profile through copper (Cu) deficiency. Eighty broiler chicks were used in the study. The chicks were separated into two equal groups (n = 40), feed treatment according to the groups started on day 7. The animals in the control group were fed with normal feed. Those in the Cu-deficient group were fed with feed that did not contain supplemental Cu. Blood samples were taken from all the animals on days 7, 21 and 49. Fibrinogen and prothrombin time were assayed in plasma; total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol (LDL-cholesterol), triglyceride and ceruloplasmin were assayed in sera; and haematocrit and thrombocyte levels were assayed in the blood samples. Total cholesterol, triglyceride and LDL-cholesterol levels were significantly higher in the Cu-deficient group than in the control group. Serum ceruloplasmin levels were lower in the Cu-deficient group than in the control group. Prothrombin time was higher in the Cu-deficient group than in the control group. Haematocrit levels were lower in the Cu-deficient group than in the control group. It was concluded that Cu deficiency created hypertriglyceridaemia, hypercholesterolaemia, insufficient growth and anaemia in broilers.

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“…Activation of T lymphocytes by oxLDL, prepared by chemical oxidation of native LDL with copper sulfate, via the lectin-type oxLDL receptor 1 (LOX-1) has also been shown to increase the expression of tissue factor on the surface of leukocytes 107 . Furthermore, oxLDL generated with copper oxidation was noted to inhibit fibrinolysis, modify fibrin clot structure and increase thrombin generation 98,108 . Finally, oxLDL (detected by 4E6) correlated to reduced clot permeability and prolonged clot lysis time 109 .…”

Section: Oxldl and Haemostasismentioning

confidence: 99%

Relationship between lipoproteins, thrombosis, and atrial fibrillation

Ding

Protty

Davies

et al. 2021

Cardiovascular Research

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The prothrombotic state in atrial fibrillation (AF) occurs as a result of multifaceted interactions, known as Virchow’s triad of hypercoagulability, structural abnormalities, and blood stasis. More recently, there is emerging evidence that lipoproteins are implicated in this process, beyond their traditional role in atherosclerosis. In this review, we provide an overview of the various lipoproteins and explore the association between lipoproteins and AF, the effects of lipoproteins on haemostasis, and the potential contribution of lipoproteins to thrombogenesis in AF. There are several types of lipoproteins based on size, lipid composition, and apolipoprotein category, namely: chylomicrons, very low-density lipoprotein, low-density lipoprotein (LDL), intermediate-density lipoprotein, and high-density lipoprotein. Each of these lipoproteins may contain numerous lipid species and proteins with a variety of different functions. Furthermore, the lipoprotein particles may be oxidized causing an alteration in their structure and content. Of note, there is a paradoxical inverse relationship between total cholesterol and LDL cholesterol (LDL-C) levels, and incident AF. The mechanism by which this occurs may be related to the stabilizing effect of cholesterol on myocardial membranes, along with its role in inflammation. Overall, specific lipoproteins may interact with haemostatic pathways to promote excess platelet activation and thrombin generation, as well as inhibiting fibrinolysis. In this regard, LDL-C has been shown to be an independent risk factor for thromboembolic events in AF. The complex relationship between lipoproteins, thrombosis and AF warrants further research with an aim to improve our knowledge base and contribute to our overall understanding of lipoprotein-mediated thrombosis.

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Effects of low-density lipoproteins on blood coagulation and fibrinolytic activity

Cited by 9 publications

References 11 publications

Interpretation and Validation of Maximum Absorbance Data Obtained from Turbidimetry Analysis of Plasma Clots

Interpretation and Validation of Maximum Absorbance Data Obtained from Turbidimetry Analysis of Plasma Clots

Effect of Copper Deficiency on Blood Lipid Profile and Haematological Parameters in Broilers

Relationship between lipoproteins, thrombosis, and atrial fibrillation

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