Effects of Long‐term Streptozotocin Diabetes on the Contractile and Histochemical Properties of Rat Muscles

Cotter, Mary A.; Cameron, Norman E.; Lean, D. R.; Robertson, S.

doi:10.1113/expphysiol.1989.sp003240

Cited by 73 publications

(104 citation statements)

References 25 publications

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“…There was a tendency for a greater reduction in this ratio in the neuropathic patients although it did not reach statistical significance. A reduced intrinsic muscle strength in the diabetic patients can be caused by impaired contractility as has been found in experimentally diabetic rats [27]. Muscle contractility in diabetic patients, however, has never been studied in detail.…”

Section: Discussionmentioning

confidence: 99%

Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study

et al. 1997

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Motor dysfunction develops in advanced cases of diabetic polyneuropathy [1,2]. In the clinic it is most frequently detected by inability to walk on heels which indicates a preponderance for the dorsal flexors of the ankle. Recently weakness of both the ankle dorsal and plantar flexors and of the knee extensors and flexors was found in long-term insulin-dependent diabetic (IDDM) patients with peripheral neuropathy at isokinetic dynamometry [3]. Furthermore, the weakness at the ankle and knee was closely related to the severity of neuropathy assessed clinically, electrophysiologically and at quantitative sensory examination.The present study was performed in order to clarify whether muscle weakness in diabetic patients with polyneuropathy is associated with muscular atrophy and secondly if present how the atrophy is topographically distributed. A non-invasive quantitative method for analysis of muscle volume was applied in the present study. Assessment of muscle morphology can be obtained with ultrasonomyography, X-ray computed tomography (CT), and magnetic resonance imaging (MRI) [4][5][6][7]. Ultrasonomyography is Diabetologia (1997) Summary Diabetic patients with polyneuropathy develop motor dysfunction. To establish whether motor dysfunction is associated with muscular atrophy the ankle dorsal and plantar flexors of the non-dominant leg were evaluated with magnetic resonance imaging in 8 patients with symptomatic neuropathy, in 8 nonneuropathic patients and in 16 individually matched control subjects. In the neuropathic patients the muscle strength of the ankle dorsal and plantar flexors was reduced by 41 % as compared to the non-neuropathic patients (p < 0.005). Volume of the ankle dorsal and plantar flexors was estimated with stereological techniques from consecutive cross-sectional images of the lower leg. The neuropathic patients had a 32 % reduction in volume as compared with the nonneuropathic patients (p < 0.005). To determine the regional distribution of atrophy cross-sectional magnetic resonance images were performed at predetermined levels of the lower leg in relation to bone landmarks. In the neuropathic patients there was an insignificant increase of 3 % of muscle area at the proximal lower leg level, whereas the atrophy was 43 % (p < 0.002) at the mid lower leg level and 65 % (p < 0.002) distally. Analysis of individual muscles confirmed that the atrophy predominated distally. We conclude that muscular atrophy underlies motor weakness at the ankle in diabetic patients with polyneuropathy and that the atrophy is most pronounced in distal muscles of the lower leg indicating that a length dependent neuropathic process explains the motor dysfunction. [Diabetologia (1997[Diabetologia ( ) 40: 1062[Diabetologia ( -1069

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Section: Discussionmentioning

confidence: 99%

Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study

et al. 1997

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“…1977;Krotkiewski and Bjorntorp 1986;Lillioja et al 1987;Gaster et al 2001;29 Oberbach et al 2006). The change in contractile properties is further described by 1 animal studies reporting a more marked diabetes-induced strength loss in fast-twitch 2 muscles (Cotter et al 1989;Sanchez et al 2005) and in skinned fast-twitch single 3 fibers (Paulus and Grossie 1983;Sanchez et al 2005) than in slow-twitch muscles. 4…”

Section: Muscle Fatigue 10mentioning

confidence: 90%

Effect of exercise training on neuromuscular function of elbow flexors and knee extensors of type 2 diabetic patients

Bazzucchi

Vito

Felici

et al. 2015

Journal of Electromyography and Kinesiology

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Massimo (2015) Effect of exercise training on neuromuscular function of elbow flexors and knee extensors of type 2 diabetic patients. Journal of Electromyography and Kinesiology, 25 (5). pp. 815-823.Downloaded from: http://insight.cumbria.ac.uk/2048/ Usage of any items from the University of Cumbria's institutional repository 'Insight' must conform to the following fair usage guidelines.Any item and its associated metadata held in the University of Cumbria's institutional repository Insight (unless stated otherwise on the metadata record) may be copied, displayed or performed, and stored in line with the JISC fair dealing guidelines (available here) for educational and not-for-profit activities provided that• the authors, title and full bibliographic details of the item are cited clearly when any part of the work is referred to verbally or in the written form• a hyperlink/URL to the original Insight record of that item is included in any citations of the work • the content is not changed in any way• all files required for usage of the item are kept together with the main item file. You may not• sell any part of an item• refer to any part of an item without citation • amend any item or contextualise it in a way that will impugn the creator's reputation• remove or alter the copyright statement on an item.The full policy can be found here. Alternatively contact the University of Cumbria Repository Editor by emailing insight@cumbria.ac.uk. Accepted Manuscript

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“…Markedly different responses to Ca 2ϩ injection in DIA vs. CONT suggest fundamentally deranged Ca 2ϩ handling. calcium buffering; Fura-2 AM; muscle contraction; spinotrapezius IN DIABETES MELLITUS (DIA), force production in skeletal muscle is impaired, while fatigability and muscle fragility are increased (4,5,14,44). Maintenance of Ca 2ϩ homeostasis across repeated muscle contractions is requisite for optimal contractile function (9).…”

Section: ϩmentioning

confidence: 99%

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

Eshima

Tanaka

Sonobe

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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([Ca 2ϩ ]i) accumulation in Type 1 diabetes are unclear. We tested the hypothesis that, following repeated bouts of muscle contractions, the rise in resting [Ca 2ϩ ]i evident in healthy rats would be increased in diabetic rats and that these changes would be associated with a decreased cytoplasmic Ca 2ϩ -buffering capacity. Adult male Wistar rats were divided randomly into diabetic (DIA; streptozotocin, ip) and healthy control (CONT) groups. Four weeks later, animals were anesthetized and spinotrapezius muscle contractions (10 sets of 50 contractions) were elicited by electrical stimulation (100 Hz). Ca 2ϩ imaging was achieved using Fura-2 AM in the spinotrapezius muscle in vivo (i.e., circulation intact). The ratio (340/380 nm) was determined from fluorescence images following each set of contractions for estimation of [Ca 2ϩ ]i. Also, muscle Ca 2ϩ buffering was studied in individual myocytes microinjected with 2 mM Ca 2ϩ solution. After muscle contractions, resting [Ca 2ϩ ]i in DIA increased earlier and more rapidly than in CONT (P Ͻ 0.05 vs. precontraction). Peak [Ca 2ϩ ]i in response to the Ca 2ϩ injection was significantly higher in CONT (25.8 Ϯ 6.0% above baseline) than DIA (10.2 Ϯ 1.1% above baseline). Subsequently, CONT [Ca 2ϩ ]i decreased rapidly (Ͻ15 s) to plateau 9 -10% above baseline, whereas DIA remained elevated throughout the 60-s measurement window. No differences in SERCA1 and SERCA2 (Ca 2ϩ uptake) protein levels were evident between CONT and DIA, whereas ryanodine receptor (Ca 2ϩ release) protein level and mitochondrial oxidative enzyme activity (succinate dehydrogenase) were decreased in DIA (P Ͻ 0.05). ] i evident in healthy rats would be magnified in muscles of diabetic rats and further that these changes would be associated with attenuation of the Ca 2ϩ -handling system within the in vivo environment. METHODS AnimalsMale Wistar rats (n ϭ 43, 10 wk of age; Japan SLC, Shizuoka, Japan) were used in this study. Rats were maintained on a 12:12-h light-dark cycle and received food and water ad libitum. Rats were divided into two groups: healthy control (CONT) and diabetic (DIA) rats. Rats were anesthetized using isoflurane and given intraperitoneal injection of 45 mg/kg body wt of streptozotocin (STZ; S0130, SigmaAldrich St. Louis, MO) prepared fresh in saline solution. CONT animals were injected with the saline solution. Urine glucose levels of rats were measured (New Uriesu Ga, Terumo, Japan) 2 days after STZ injection with the onset of diabetes raising glucose concentrations above 500 mg/dl. These measurements of urine glucose were continued each week for 4 wk. At experiment completion, blood was collected from a tail vein puncture to confirm that the blood glucose level exceeded 300 mg/dl. All experiments were conducted under the guidelines established by the Physiological Society of Japan and were approved by University of Electro-Communications Institutional Animal Care and Use Committee. The rats were anesthetized using pentobarbital sodium (60 mg/kg ip), and supplemental dose...

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Effects of Long‐term Streptozotocin Diabetes on the Contractile and Histochemical Properties of Rat Muscles

Cited by 73 publications

References 25 publications

Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study

Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study

Effect of exercise training on neuromuscular function of elbow flexors and knee extensors of type 2 diabetic patients

In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes

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Effects of Long‐term Streptozotocin Diabetes on the Contractile and Histochemical Properties of Rat Muscles

Cited by 73 publications

References 25 publications

Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study

Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study

Effect of exercise training on neuromuscular function of elbow flexors and knee extensors of type 2 diabetic patients

In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes

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In vivo imaging of intracellular Ca²⁺ after muscle contractions and direct Ca²⁺ injection in rat skeletal muscle in diabetes