Effects of locally applied nerve growth factor to the inferior alveolar nerve histology in a rabbit model of mandibular distraction osteogenesis

Wang, L.; Zhao, Yinghua; Cheng, Xigao; Yang, Yongheng; Liu, G.; Ma, Qiang; Shang, Haitao; Tian, Li; Lei, Delin

doi:10.1016/j.ijom.2008.11.010

Cited by 19 publications

(9 citation statements)

References 25 publications

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“…Histomorphometric studies also showed that IAN regeneration with an NGF-supplying implant could enhance functional recovery with meaningfully larger total numbers of axons and axon density, as shown by other studies. 26,27,38 In addition, although there was no important difference between the 2 groups, the mean value of the G-ratio in the NGF group was close to 0.6, which is considered the optimal value for the FIGURE 7. Histomorphometric analysis of myelin (magnification, Â5,000).…”

Section: Discussionmentioning

confidence: 77%

Simultaneous Inferior Alveolar Nerve Regeneration and Osseointegration With a Nerve Growth Factor-Supplying Implant: A Preliminary Study

Lee

Jahng

Kim

et al. 2015

Journal of Oral and Maxillofacial Surgery

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Section: Discussionmentioning

confidence: 77%

Simultaneous Inferior Alveolar Nerve Regeneration and Osseointegration With a Nerve Growth Factor-Supplying Implant: A Preliminary Study

Lee

Jahng

Kim

et al. 2015

Journal of Oral and Maxillofacial Surgery

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“…Byun et al (2008) observed the presence of NGF in the inferior alveolar nerve during mandible lengthening. Local application of NGF enhances recovery of inferior alveolar nerve and bone formation during mandibular DO (Wang et al, 2009a(Wang et al, , 2009bDu et al, 2011) Based on these findings, it can be inferred that similarities should exist in NGF secretion in the distracted tibial nerve during the limb lengthening. To elucidate whether this occurs in DO, it is helpful to have an insight as to the timing of cytokine secretion in the distracted limb nerves.…”

Section: Discussionmentioning

confidence: 92%

Expression of Nerve Growth Factor and its Receptor in Distracted Tibial Nerve after Limb Lengthening

Shao

HengSheng

Wang³

et al. 2012

The Anatomical Record

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Despite many experimental and clinical studies conducted on distraction osteogenesis (DO) in the past decade, changes in the surrounding tissues that occur after the procedure remains poorly understood. To study the biochemical changes of recovery in nerve tissues upon DO-induced nerve injury, we prepared a rabbit model of tibia lengthening to observe the expression pattern of nerve growth factor (NGF) and low-affinity NGF receptor (p75NGFR) in the distracted tibial nerve. The distracted tibial nerve was harvested at various time points during the consolidation period of new bone formation and immunohistochemical staining was performed to detect the expression of NGF and p75NGFR. The expression levels of NGF and p75NGFR were found to be different at various times after DO. The changes in expression of these two cellular factors show similar tendencies with significantly elevated expression in Schwann cells at 7 and 14 days after distraction, but low or undetectable levels of expression at 0, 28, and 56 days. These results suggest that NGF and p75NGFR may play important roles in the adaptive process of the distracted nerve. NGF and p75NGFR are autocrine growth factors present in the distracted nerve during the early consolidation period. NGF interacts with p75NGFR to promote damage repair and reconstruction of nerves. Together, this study furthers the understanding of the relative mechanisms of nerve repair, as well as provides a further basis for the clinical application of neurotrophins. Anat Rec, 296:333-339, 2013. V C 2012 Wiley Periodicals, Inc.

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“…The NGF or nerotrophic factor is secreted from the Schwann cell 14) ; since the level of secretion is insufficient or inconsistent during the period of 4 to 8 weeks after injury, however, healing may be accelerated if the NGF is supplied from the outside 9,15) . Ultimately, the problem to be solved is the method of supplying an appropriate dose of NGF on a timely basis.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in selecting the supplying method, appropriate measures should be considered to ensure that the externally supplied NGF retains activity without being biodegraded. Wang et al 14) directly injected NGF many times into the lower jaw of the rabbit, which was sutured after undergoing distraction osteogenesis, to attempt nerve regeneration. This method is simple but carries demerits, e.g., it might leave scars after numerous injections, and it is impossible to verify whether an appropriate dose of NGF is being applied for an appropriate duration to the damaged part.…”

Section: Discussionmentioning

confidence: 99%

Animal Model for the Evaluation of Repair of Injured Inferior Alveolar Nerve with Nerve Growth Factor

Lee¹,

Park²,

Heo³

et al. 2013

Journal of Korean Dental Science

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The inferior alveolar nerve (IAN) can be damaged as a result of minor oral surgical procedure such as third molar extraction or implant placement. Repair of the injured IAN involves difficulty of access, and research studies are limited to elucidating the process of regeneration by surgical methods. This study sought to establish the rabbit animal model to apply polymeric membrane functionalized with nerve growth factor after a crush lesion for the evaluation of nerve regeneration using the electrophysiologic method. Materials and Methods: The IAN of 2 adult male New Zealand white rabbits (4 nerves) were exposed bilaterally, and crush injury rendered by jeweler's forceps was applied. Nerve conduction velocity was examined electrophysiologically using electromyography before, after, and 4 weeks after the crush injury. To evaluate the regeneration, the pattern of action potential of IAN was recorded, and the characteristics of neurons were histologically observed. Result: After the crush injury, afferent activity decreased in the injured group. Electromyography could not be recorded after four weeks because tissues surrounding the injured nerve collapsed. Decrease in the mean number of axons was observed in the injured part with membrane. Conclusion: Despite the limited result, the present animal model study may provide a possible way to research on the methods of enhancing the recovery of nerve injuries in clinical situations. For clinically widespread acceptance, however, it should gain more consecutive and scientific evidences.

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Effects of locally applied nerve growth factor to the inferior alveolar nerve histology in a rabbit model of mandibular distraction osteogenesis

Cited by 19 publications

References 25 publications

Simultaneous Inferior Alveolar Nerve Regeneration and Osseointegration With a Nerve Growth Factor-Supplying Implant: A Preliminary Study

Simultaneous Inferior Alveolar Nerve Regeneration and Osseointegration With a Nerve Growth Factor-Supplying Implant: A Preliminary Study

Expression of Nerve Growth Factor and its Receptor in Distracted Tibial Nerve after Limb Lengthening

Animal Model for the Evaluation of Repair of Injured Inferior Alveolar Nerve with Nerve Growth Factor

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