Effects of Local Administration of Propolis and Its Polyphenolic Compounds on Tumor Formation and Growth

Oršolić, Nada; Terzić, Svjetlana; Mihaljević, Željko; Šver, Lidija; Basić, Ivan

doi:10.1248/bpb.28.1928

Cited by 67 publications

(72 citation statements)

References 32 publications

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“…The dose chosen for our experiment (150 mg/kg body weight) was based on previous studies by other researchers investigating the anticarcinogenic effects of WSDP extracted from propolis collected in Minas Gerais 5,7,8 . Three amino acids have been described as promoting bladder cancer, namely DL tryptophan, L-leucine and L-isoleucine 26,27 .…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have reported anti-infectious 3 , antiinflammatory 4 , immunomodulatory 5,6 , antitumor 7 , antioxidant [8][9][10] and antimetastatic 5 effects of WSDP. The discovery of the anticarcinogenic effects of propolis encouraged efforts to identify, isolate and synthesize constituents of propolis and to study their effects and mechanisms of action, including artepillin C 11 , caffeic acid 12 , caffeic acid phenethyl ester 13 , and in the present study, we used an in vivo model to evaluate the effect of intragastric administration of green propolis (in the form of WSDP) on angiogenesis in BBN-induced rat bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

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Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To determine the effects of water-soluble derivative of green propolis in bladder cancer angiogenesis in rats given N-butyl-(-4-hydroxybutyl) nitrosamine (BBN). METHODS:Nine groups were established, where six of them (Groups 1 to 6), the animals received 0.05% BBN in their drinking water for 14 weeks. From the 32nd to the 40th week, Groups 1, 2, 3 and 4 were treated respectively with water, L-lysine (300 mg/kg/ day), celecoxib (30 mg/kg/day) and propolis (300 mg/kg/day). Groups 5 and 6 were given propolis and L-lysine from the 1st to the 40th week (150 mg/kg/day). Microvascular density was determined by histological sections stained for the marker CD-31 and analyzed with specific software. RESULTS:The microvascular density in bladder carcinomas was lower (p<0.01) in rats receiving propolis than in controls given carcinogen only. On the other hand, the microvascular density of tumors in rats receiving carcinogen and L-lysine for 40 weeks from the beginning of carcinogen treatment was significantly higher (p<0.01) than in the corresponding controls. -Acta Cirúrgica Brasileira -Vol. 27 (8) 2012de beber por 14 semanas. Na 32ª semana das 40 semanas, os grupos 1, 2, 3 e 4 foram tratados respectivamente com água, L lisina (300 mg/kg/dia), celecoxibe (30 mg/kg/dia) e própolis (300 mg/kg/dia). Os grupos 5 e 6 receberam própolis e L lisina da 1ª a 40ª semana (150 mg/ kg/dia). A densidade microvascular foi determinada por cortes histológicos corados pelo CD-31 e analisados por programa de computador específico. RESULTADOS:A densidade microvascular em carcinomas de bexiga foi menor com p<0,01 nos ratos que receberam própolis do que nos carcinomas do grupo controle que recebeu apenas carcinógeno. Por outro lado, a densidade microvascular de tumores de ratos que receberam carcinógeno e L-Lisina por 40 semanas desde o início do carcinógeno foi significantemente maior com p<0,01 que a densidade microvascular dos tumores de seu respectivo grupo controle. CONCLUSÃO:A própolis verde solúvel em água inibiu a angiogênese em câncer de bexiga induzido pelo BBN, enquanto a L-lisina estimulou a angiogênese quando iniciada juntamente com o BBN.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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“…Propolis not only from Brazil but other countries is studied in parallel with compounds of pharmacological interest isolated from them, such as artepilin C, CAPPE (caffeic acid phenethyl ester), p-coumaric acid, caffeic acid, quercetin, chrysin and naringenin, showing reduction of tumors in animals and cytotoxic effects in cultured human tumor cells [21][22][23][24][25] .…”

Section: Discussionmentioning

confidence: 99%

Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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-ORIGINAL ARTICLEEXPERIMENTAL ABSTRACT PURPOSE:To determine the effects of green propolis extracted in L-lysine (WSDP) and of L-lysine for 40 weeks on induced rat bladder carcinogenesis. METHODS:The animals (groups I, II, III, IV, V and VI) received BBN during 14 weeks. Group I was treated with propolis 30 days prior received BBN, and then these animals were treated daily with propolis; Groups II and III was treated with subcutaneous and oral propolis (respectively) concurrently with BBN. The animals of Group IV were treated L-lysine; Group V received water subcutaneous;and Group VI received only to BBN. Among the animals not submitted to carcinogenesis induction, Group VII received propolis, Group VIII received L-lysine and Group IX received water. RESULTS:The carcinoma incidence in Group I was lower than that of control (Group VI). The carcinoma multiplicity in Group IV was greater than in Group VI. All animals treated with L-lysine developed carcinomas, and they were also more invasive in Group IV than in controls. On the other hand, Group VIII showed no bladder lesions. CONCLUSION:The WSDP is chemopreventive against rat bladder carcinogenesis, if administered 30 days prior to BBN , and that L-lysine causes promotion of bladder carcinogenesis.

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“…Já polifenólicos, como o ácido caféico e o cafeato de feniletila, que são os dois componentes mais ativos da própolis de Apis mellifera, afetam o crescimento do tumor através da inibição da síntese de DNA e exercem efeitos antitumorais através da indução de apoptose das células tumorais (Orsolic et al, 2005b). O aumento dos índices de apoptose tem sido associado à inibição do crescimento de células potencialmente oncogênicas (Campisi, 2005), porém, o mecanismo envolvido na ação antitumoral da própolis não está completamente elucidado.…”

Section: Discussionunclassified

Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos

Araújo¹,

Dutra²,

Costa³

et al. 2010

Rev. bras. farmacogn.

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RESUMO:Neste trabalho foi investigado o efeito do extrato hidroalcoólico de própolis (EHP) de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich na forma sólida, sobre a celularidade dos órgãos linfóides dos animais portadores de tumor, bem como, sobre a produção de óxido nítrico (NO) pelos macrófagos destes animais. Camundongos Swiss foram divididos em quatro grupos: controle, EHP 0,5; EHP 5 e EHP 50, os quais foram tratados por via intraperitoneal com dose única de solução salina (NaCl 0,9%); 0,5; 5 ou 50 mg de EHP/kg de animal, respectivamente. Depois de 48 h do tratamento, os animais foram inoculados com 10 5 células do tumor de Ehrlich nas patas. Os resultados mostraram que o tratamento com EHP nas doses de 5 e 50 mg/kg inibiu de forma significativa o desenvolvimento do tumor a partir do 6º dia pós-inóculo quando comparado ao controle e ao EHP 0,5. Além disso, houve aumento significativo da celularidade do baço e da medula óssea nos grupos EHP 0,5 e EHP 5 em relação ao controle. A produção de NO estimulada com concanavalina A (ConA) apresentou uma significante diminuição nos grupos tratados com EHP em relação ao controle. Pode-se concluir que o tratamento com EHP apresentou efeito antitumoral quando administrado nas doses de 5 e 50 mg/kg, o que pode estar relacionado com a sua composição química e com a inibição da produção de NO.Unitermos: própolis, Scaptotrigona aff. postica, tumor de Ehrlich, abelhas sem ferrão, Meliponinae.ABSTRACT: "Effect of propolis of Scaptotrigona aff. postica on the development of the tumor of Ehrlich in mice". It was investigated the effect of hydroalcoholic extract (HEP) of propolis from Scaptotrigona aff. postica on the solid Ehrlich tumor, on the tumor-bearing mice lymphoid organs and on the nitric oxide (NO) production. Swiss mice were divided in 4 groups: control, HEP 0.5; HEP 5 and HEP 50 that was treated by intraperitoneal route with a single dose of saline solution (NaCl 0.9%) or 0.5 or 5 or 50 mg of HEP/kg body weight, respectively. After 48 h of treatment, the animals were inoculated with 10 5 tumor cells in their footpad. The results showed that the treatment with HEP in the doses of 5 and 50 mg/kg inhibited the development of the tumor from the 6 th day post inoculums when compared to the control and to the HEP 0.5 groups. Besides, there was an increase of spleen and bone marrow cell number in HEP 0.5 and HEP 5 as compared to the control. Concanavalin A (ConA)-stimulated NO production was decreased in all HEP-treated groups when compared to the control. In conclusion, the treatment with HEP had an anti-tumor effect what may be related to its chemical composition and to the inhibition of NO production.

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Effects of Local Administration of Propolis and Its Polyphenolic Compounds on Tumor Formation and Growth

Cited by 67 publications

References 32 publications

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos

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