Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

Dornelas, Conceição Aparecida; Fechine-Jamacaru, Francisco Vagnaldo; Albuquerque, Irineu Lima; Magalhães, Hemerson Iury Ferreira; Souza, Adjair Jairo Silva de; Alves, Leonardo Alcântara; Almeida, Paulo Roberto Carvalho de; Lemos, Telma L. G.; Castro, Jaelson; Moraes, Maria Elisabete Amaral de; Moraes, Manoel Odoríco de

doi:10.1590/s0102-86502012000200015

Cited by 18 publications

(22 citation statements)

References 18 publications

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“…In paper accept for publication, based on the same experimental model shows that L-lysine was able to stimulate carcinoma multiplicity and invasion in BBN-induced rat bladder cancer 28 .…”

Section: Discussionmentioning

confidence: 97%

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To determine the effects of water-soluble derivative of green propolis in bladder cancer angiogenesis in rats given N-butyl-(-4-hydroxybutyl) nitrosamine (BBN). METHODS:Nine groups were established, where six of them (Groups 1 to 6), the animals received 0.05% BBN in their drinking water for 14 weeks. From the 32nd to the 40th week, Groups 1, 2, 3 and 4 were treated respectively with water, L-lysine (300 mg/kg/ day), celecoxib (30 mg/kg/day) and propolis (300 mg/kg/day). Groups 5 and 6 were given propolis and L-lysine from the 1st to the 40th week (150 mg/kg/day). Microvascular density was determined by histological sections stained for the marker CD-31 and analyzed with specific software. RESULTS:The microvascular density in bladder carcinomas was lower (p<0.01) in rats receiving propolis than in controls given carcinogen only. On the other hand, the microvascular density of tumors in rats receiving carcinogen and L-lysine for 40 weeks from the beginning of carcinogen treatment was significantly higher (p<0.01) than in the corresponding controls. -Acta Cirúrgica Brasileira -Vol. 27 (8) 2012de beber por 14 semanas. Na 32ª semana das 40 semanas, os grupos 1, 2, 3 e 4 foram tratados respectivamente com água, L lisina (300 mg/kg/dia), celecoxibe (30 mg/kg/dia) e própolis (300 mg/kg/dia). Os grupos 5 e 6 receberam própolis e L lisina da 1ª a 40ª semana (150 mg/ kg/dia). A densidade microvascular foi determinada por cortes histológicos corados pelo CD-31 e analisados por programa de computador específico. RESULTADOS:A densidade microvascular em carcinomas de bexiga foi menor com p<0,01 nos ratos que receberam própolis do que nos carcinomas do grupo controle que recebeu apenas carcinógeno. Por outro lado, a densidade microvascular de tumores de ratos que receberam carcinógeno e L-Lisina por 40 semanas desde o início do carcinógeno foi significantemente maior com p<0,01 que a densidade microvascular dos tumores de seu respectivo grupo controle. CONCLUSÃO:A própolis verde solúvel em água inibiu a angiogênese em câncer de bexiga induzido pelo BBN, enquanto a L-lisina estimulou a angiogênese quando iniciada juntamente com o BBN.

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“…In paper accept for publication, based on the same experimental model shows that L-lysine was able to stimulate carcinoma multiplicity and invasion in BBN-induced rat bladder cancer 28 .…”

Section: Discussionmentioning

confidence: 97%

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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“…This work is a continuation of earlier studies published by Dornelas et al 7,9 whose objective was to evaluate the effect of green propolis and L-lysine on carcinogenesis and angiogenesis in rats subjected to chemical carcinogenesis of the bladder. In the earlier studies, we found that propolis administered to rats inhibited angiogenesis and bladder carcinogenesis in this model at the doses studied and that L-lysine promoted bladder carcinogenesis.…”

Section: Discussionmentioning

confidence: 76%

“…MNT is utilized to detect chromosomal damage of the mitotic spindle, in which during the maturation of erythroblasts, the nucleus is expelled from the cell and in the absence of damage through breaks or damage in the mitotic spindle, where no residual chromatid (micronucleus) remains inside the cell. Propolis is a resin produced by bees that has various antineoplasic 2 , antiinflammatory 3 , immunomodulatory 4 , antibacterial 5 and antiangiogenesis properties 6,7 . L-lysine is an essential amino acid and has been utilized in the extraction of propolis since the patented proposal of Nicolov et al 8 up to recent publications 2,3,7,9 .…”

Section: Introductionmentioning

confidence: 99%

“…Propolis is a resin produced by bees that has various antineoplasic 2 , antiinflammatory 3 , immunomodulatory 4 , antibacterial 5 and antiangiogenesis properties 6,7 . L-lysine is an essential amino acid and has been utilized in the extraction of propolis since the patented proposal of Nicolov et al 8 up to recent publications 2,3,7,9 . The objective of this work was to study the genotoxicity of green propolis and L-lysine on the bone marrow of rats subjected to chemical carcinogenesis of the bladder.…”

Section: Introductionmentioning

confidence: 99%

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Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

et al. 2014

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PURPOSE:To evaluate the genotoxicity of propolis and L-lysine, as well as their effects on the possible cellular damage in erythroblasts (bone marrow) and leukocytes (peripheral blood) caused by the carcinogen BBN (n -butyl -n {4 -hydroxybutyl} nitrosamine) in rats subjected to bladder carcinogenesis and treated with green propolis and L-lysine. METHODS:One hundred and twenty five rats were distributed into the following groups: I, IIA, IIB, III, K, L M N, X, XI, XII and XIII. Groups I to X received BBN in drinking water for 14 weeks (wks). Group I was treated with intragastric (ig) propolis at 150 mg/ kg body weight, for 44 wks, beginning 30 days before start of BBN. Groups IIA and III were treated with propolis (150 mg/kg), for 40 wks, subcutaneous (sc) and ig, respectively, beginning simultaneously with BBN. On the 32nd wk, the animals of groups L, M and N were treated ig with L-lysine (300 mg/kg), celecoxib (30 mg/kg) and propolis (300 mg/kg), respectively, up to the 40th wk. The groups that received only BBN (IIB and K) were treated with water, sc and orally, respectively, for 40 wks. Groups XI, XII and XIII received respectively propolis (150 mg/kg), L-lysine (150 mg/kg) and water ig for 40 wks. After 40 wks, the surviving animals were anesthetized and subjected to femoral bone marrow aspiration and blood collection from the aorta, for CA and MNT, respectively, for investigation of genotoxicity.RESULTS: Groups IIB and K, which received only BBN and water, showed the greatest DNA damage in peripheral leukocytes (CA) and largest number of micronuclei in bone marrow erythrocytes (MNT) in relation to all other groups that received BBN and lysine and/ or propolis (p<0.001). CONCLUSIONS:Both propolis and L-lysine are effective in protecting against genotoxicity, as well not being genotoxic themselves toward the cells evaluated, at the doses and times administered and according to the two tests utilized.

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“…In a recent study on rats submitted to chemical carcinogenesis of the bladder, L-lysine was found to promote, but not initiate, bladder cancer 6 .…”

Section: ■ Introductionmentioning

confidence: 94%

Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L -lysine

Santos

Coelho²,

Azevedo³

et al. 2017

Acta Cir. Bras.

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Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L-lysine 1 5-Experimental SurgeryActa Cir Bras. 2017;32(3):297-306 Abstract Purpose: To evaluated the effects of L-lysine on the intestinal and urothelial epithelia in cystoplasty in rats. Methods: Twenty-eight 9-week-old rats were assigned to 4 groups: Group A (n=8) cystoplasty followed by administration of L-lysine (150 mg/kg body weight by gavage) for 30 weeks; Group B (n=8) cystoplasty + water for 30 weeks; Group C (n=6) L-lysine for 30 weeks; Group D (n=6) water for 30 weeks.Results: On histopathology with hematoxylin and eosin, mild to moderate hyperplasia transitional was observed in at the site of anastomosis in all animals submitted to cystoplasty (Groups A and B), but "transitional metaplasia" of the intestinal glandular epithelium was more accentuated in Group A (p=0.045). No inflammatory cells, dysplasia or abnormalities were observed. Staining with Alcian blue revealed a substantial reduction of goblet cells and mucins in the colon segment (Groups A and B). Conclusion:The administration of L-lysine to rats accelerated the development of transitional metaplasia in the epithelium of the colon segment in cystoplasty.

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Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

Cited by 18 publications

References 18 publications

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

Transitional metaplasia in intestinal epithelium of rats submitted to intestinal cystoplasty and treatment with L -lysine

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