Effects of Liposomes of Different Lipid Composition on In Vitro Growth of Mycobacterium Tuberculosis H37Rv

Abstract

The growth of M. tuberculosis H37RV in culture medium was studied after addition of liposomes from different lipids (phosphatidylcholine, cardiolipin, and glycosphyngolipids). Addition of phosphatidylcholine into culture medium did not modify the growth and multiplication of mycobacteria. Addition of glycosphyngolipids and their mixture with phosphatidylcholine partially inhibited the growth. Addition of cardiolipin inhibited the growth of mycobacteria and even suppressed it, depending on the dose. Presumably, high concentrations of cardiolipin added into the culture medium, can transfer the mycobacteria into an uncultivable state.

Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli

“…Applying Murohashi staining method we demonstrated that such concentration of CL cause lysis of M. tuberculosis H37Rv cells (fig. 5) [Sorokoumova, 2009]. Fig.…”

“…Reproduced from [Sorokoumova, 2009] # Lipid 2-H37Rv+CL (50 µg/ml); 3-H37Rv+CL (750 µg/ml). Reproduced from [Mikulovish, 2010] These data correlate well with the results of mycobacterial DNA detection by real time PCR, according to which appearance CL in culture medium didn't lead to increase of DNA number [Sorokoumova, 2009] (fig. 6).…”

Lipid Surrounding of Mycobacteria: Lethal and Resuscitating Effects

“…Applying Murohashi staining method we demonstrated that such concentration of CL cause lysis of M. tuberculosis H37Rv cells ( fig. 5) [Sorokoumova, 2009]. Reproduced from [Sorokoumova, 2009] 2-H37Rv+CL (50 µg/ml); 3-H37Rv+CL (750 µg/ml).…”

“…Reproduced from [Sorokoumova, 2009] 2-H37Rv+CL (50 µg/ml); 3-H37Rv+CL (750 µg/ml). Reproduced from [Mikulovish, 2010] These data correlate well with the results of mycobacterial DNA detection by real time PCR, according to which appearance CL in culture medium didn't lead to increase of DNA number [Sorokoumova, 2009] (fig. 6).…”

“…Reproduced from [Sorokoumova G.M., 2009] CL is an instable compound that transforms in several substances in water phase. Both in Dubo's medium, containing high concentration of iron ions, and in Tris-buffer, which doesn't have an iron, the products of hydrolysis were mainly formed: lyso-, bislysocomponents and FA ( fig.…”