Effects of lipopolysaccharides on human dental pulp cells

Nakane, Akinobu; Yoshida, Tsutomu; Nakata, Kohei; Horiba, Naoki; Nakamura, Hiroshi

doi:10.1016/s0099-2399(06)80437-1

Cited by 25 publications

(22 citation statements)

References 15 publications

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“…The data presented here showed that P. intermedia LPS induced a similar trend of VEGF induction to E. coli LPS, but it required higher concentrations of LPS. A previous investigation has evaluated the effect of LPS from Porphyromonas endodontalis, Porphyromonas gingivalis, Fusobacterium nucleatum, and E. coli on DNA content, protein synthesis, and alkaline phosphatase activity in dental pulp cells (44). Other studies have used a range of 0.01-100 lg ml )1 LPS (27,44), which is believed to be within a range of LPS relevant to pulp pathologies (45).…”

Section: Discussionmentioning

confidence: 99%

Effect of lipopolysaccharides on vascular endothelial growth factor expression in mouse pulp cells and macrophages

Botero¹,

Mantellini²,

Song³

et al. 2003

European J Oral Sciences

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Vascular endothelial growth factor (VEGF), a potent pro-angiogenic factor, might regulate the neovascularization observed in the pulp of teeth with deep caries. The purpose of this in vitro study was to evaluate the effect of bacterial lipopolysaccharides (LPS) on VEGF expression in dental pulp cells. Mouse odontoblast-like cells (MDPC-23) or undifferentiated pulp cells (OD-21) were exposed to 0-20 microg ml-1Escherichia coli LPS or 0-80 microg ml-1Prevotella intermedia LPS. As controls, mouse macrophages or gingival fibroblasts were exposed to LPS, since these cells are known to secrete VEGF. The VEGF expression was evaluated by reverse transcriptase polymerase chain reaction or enzyme-linked immunosorbent assay. The baseline expression levels of VEGF protein were higher in MDPC-23 and OD-21 than in fibroblasts or macrophages. Vascular endothelial growth factor protein expression was upregulated in MDPC-23 and macrophages exposed to E. coli LPS, but not in OD-21 cells or fibroblasts. Higher concentrations of P. intermedia LPS were required to induce VEGF expression in MDPC-23 cells. Treatment with LPS did not affect VEGF expression at the mRNA level in any of the cells evaluated. These results demonstrate that bacterial LPS upregulates VEGF expression in odontoblast-like cells and macrophages, and suggest that the regulation of VEGF expression occurs primarily at a post-transcriptional level.

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Section: Discussionmentioning

confidence: 99%

Effect of lipopolysaccharides on vascular endothelial growth factor expression in mouse pulp cells and macrophages

Botero¹,

Mantellini²,

Song³

et al. 2003

European J Oral Sciences

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show abstract

“…Bacteria that invade the dentin as well as their products that diffuse through dentinal tubules are involved in the pathogenesis of pulpitis (2,3). Lipopolysaccharide (LPS) is a major cell wall component in gram-negative bacteria that has been shown to be a potent inducer of pulpitis (4).…”

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confidence: 99%