Effects of Linderae radix extracts on a rat model of alcoholic liver injury

Abstract

Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of Lindera aggregata (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol-induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin-eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription-quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF-κB, TNF-α and IL-1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action.

Polydatin Protects Rat Liver against Ethanol‐Induced Injury: Involvement of CYP2E1/ROS/Nrf2 and TLR4/NF‐κB p65 Pathway

“…When stimulated, NF- κ B p65 will bind to DNA and trigger the production of inflammatory mediators, such as TNF- α , IL-1 β , and IL-6. Accumulation of TNF- α , IL-1 β , and IL-6 are thought to be the key factors in the pathogenesis of ALD because of the disruption of the balance between proinflammation and anti-inflammation [ 57 – 60 ]. Therefore, suppression of the inflammatory reaction is a crucial strategy to ameliorate ethanol-induced liver injury.…”

“…Recently, it is reported that nuclear factor erythroid 2-related factor 2 (Nrf2) could protect against CYP2E1-induced oxidative stress through upregulation of antioxidant enzymes like HO-1 [ 12 ]. In addition, accumulating evidence has shown that chronic excessive alcohol intake also increases the level of inflammatory cytokines, such as TNF- α , IL-1 β , and IL-6 [ 13 ]. The release of these cytokines is mainly associated with the activation of toll-like receptor 4 (TLR4) and its downstream nuclear factor kappa B (NF- κ B), which remains the key inflammatory pathway playing a vital role in alcohol-induced ALD model [ 14 ].…”

Molecular mechanism and research progress on pharmacology of traditional Chinese medicine in liver injury

“…Due to its unique propensity, d -GalN has been widely used in inflammatory liver injury models to screen for potential hepatoprotective agents (Wang et al. 2016 ).…”

“…Diels (Umbelliferae) (Wang et al. 2016 ), and Dracocephalum heterophyllum Benth. (Labiatae) (Zheng et al.…”

Extraction and Isolation of Kaempferol Glycosides from the Leaves and Twigs of Lindera neesiana

“…The genus Lindera (Family: Lauraceae) consists of about 100 species widely distributed in tropical, subtropical, and temperate zones of Asia and Midwestern America [1]. Various species of Lindera plants are used in traditional medicine for the treatment of different ailments including rheumatoid arthritis, stroke, cholera, diarrhea, toothache, and nausea [1][2][3]. Lindera plants are a rich source of fatty and essential oils that are used for the production of soaps, lubricants, spices, fragrances, and timber [1].…”